Experience

Bio2Dental

• Netherlands
• Medical Practices
• 1 - 100 Employee

• Moleculair Microbiologisch analist

  • Apr 2021 - Present



Byondis

• Netherlands
• Pharmaceutical Manufacturing
• 200 - 300 Employee

• Microbiologisch Analist

  • Nov 2020 - Mar 2021



Eurofins Agro & Horti NL

• Netherlands
• Research Services
• 100 - 200 Employee

• Microbiologisch laborant

  • May 2019 - Apr 2020



Limgroup

• Netherlands
• Farming
• 1 - 100 Employee

• Moleculair Genetisch Analist

  • Feb 2018 - Jan 2019

  Carry out DNA isolations (CTAB, magnetic-beads based kits), PCRs, HRM, KASP assays to control the genetic purity of plant material (Asparagus and Strawberry) for the breeding and seeds production departments. Carry out DNA isolations (CTAB, magnetic-beads based kits), PCRs, HRM, KASP assays to control the genetic purity of plant material (Asparagus and Strawberry) for the breeding and seeds production departments.



Radboud Institute for Molecular Life Sciences (RIMLS)

• Netherlands
• Research Services
• 1 - 100 Employee

• Research Technician at the Department of Medical Microbiology (Parasitology), RadboudUMC

  • Sep 2016 - Dec 2016

  Performing DNA/RNA isolation, qPCR and RT-qPCR during a Controlled Human Malaria Infection (CHMI) model study.and for different others studies (infected populations in malaria endemic countries) for better comprehension of malaria transmission. Performing DNA/RNA isolation, qPCR and RT-qPCR during a Controlled Human Malaria Infection (CHMI) model study.and for different others studies (infected populations in malaria endemic countries) for better comprehension of malaria transmission.



KeyGene

• Netherlands
• Biotechnology Research
• 100 - 200 Employee

• Technician at Technology & Traits Mechanisms department

  • Sep 2006 - Apr 2015

  In TTM, I participated in several large research projects as EU-PEARLS project for which I screened genomic BAC libraries for rubber biosynthesis genes, and Apomixis project where I participated in the fine mapping of DIP and PAR genes in Taraxacum. Lately, as a member of the Research Support Group, I performed DNA/RNA isolations, company-wide projects. Furthermore, I worked on High-Throughput Genotyping projects combined with Next Generation Sequencing technologies.At the same time, as a member of the Molecular Mutagenesis group, I was involved in most stages of Keypoint® Mutation Breeding projects on multiple crops (tomato, pepper to name a few), including generation of mutated populations, gen-specific amplification, sequence analysis after deep sequencing of 3D pools and mutation validation. Besides, I was responsible of the Oligo-order service.

• Technician at Applied Research department

  • Mar 2005 - Aug 2006

  As a member of the AR department, I worked in close collaboration with project leaders on numerous projects, mainly Marker Assisted Breeding applications like Genetic Distance Analysis, Bulk Segregant Analysis and Visualization of markers. Besides, I trained and advised colleagues for the construction of BAC libraries.

• Technician at Upstream Research department

  • Jun 1999 - Feb 2005

  During this period, I constructed large insert BAC libraries of many genomes (Solanaceae, Maize, Zebra fish) and successfully implemented different steps of the procedure, from HMW DNA and vector preparation to BAC clones analysis. I also worked for the High-Throughput Genotyping program performing SNP discovery/validation on different crops. I also worked on the implementation of Tilling as a routine screening method for the detection of single nucleotide mutations in pooled samples of a mutants population.



GENSET S.A

• Research Assistant

  • Apr 1996 - Sep 1998

  Construction and Handling of large Genset Human BAC libraries in the Physical Mapping team, for the construction of a map of Single Nucleotide Polymorphisms as a tool to accelerate the discovery of genes associated with diseases, Construction and Handling of large Genset Human BAC libraries in the Physical Mapping team, for the construction of a map of Single Nucleotide Polymorphisms as a tool to accelerate the discovery of genes associated with diseases,



CEPH - Fondation Jean Dausset

• Biotechnology
• 1 - 100 Employee

• Research Assistant

  • Oct 1990 - Mar 1996

  DNA isolation and controls from 60 reference families and sending to worldwide collaborators laboratories.Yeast Artificial Chromosome (YAC) and Bacterial Artificial Chromosome (BAC) libraries construction, Techniques; Lymphoblastoïde cell culture, DNA isolation, RFLP, Southern blot,32P hybridization, FIGE and CHEF electrophoresis, restriction, cloning (transformation, electroporation),3D-PCR screening, DNA isolation and controls from 60 reference families and sending to worldwide collaborators laboratories.Yeast Artificial Chromosome (YAC) and Bacterial Artificial Chromosome (BAC) libraries construction, Techniques; Lymphoblastoïde cell culture, DNA isolation, RFLP, Southern blot,32P hybridization, FIGE and CHEF electrophoresis, restriction, cloning (transformation, electroporation),3D-PCR screening,