Sandra Augustin

Technische Assistentin at Humboldt-Universität zu Berlin
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Contact Information
us****@****om
(386) 825-5501
Location
Berlin Metropolitan Area, DE

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Experience

    • Germany
    • Higher Education
    • 700 & Above Employee
    • Technische Assistentin
      • Sep 2018 - Present

      cultivation of stable cell lines (HEK-293, ND7/23) and their preparation and performance of electrophysiological experiments (transfection, patch-clamp), cloning (e.g. Quick-change, Gibson, Transformation); protein purification (HEK-T cells) cultivation of stable cell lines (HEK-293, ND7/23) and their preparation and performance of electrophysiological experiments (transfection, patch-clamp), cloning (e.g. Quick-change, Gibson, Transformation); protein purification (HEK-T cells)

    • Research Services
    • 1 - 100 Employee
    • Biologielaborantin
      • Aug 2016 - Jul 2018

      cultivation of pathogenic bacteria, cloning procedure including mutant construction, expression and purification of proteins, biochemical assays, protein and RNA-isolation, preparation of samples for massspectronomy and next-generation sequencing cultivation of pathogenic bacteria, cloning procedure including mutant construction, expression and purification of proteins, biochemical assays, protein and RNA-isolation, preparation of samples for massspectronomy and next-generation sequencing

  • Leibniz Institut für Zoo und Wildtierforschung
    • Berlin und Umgebung, Deutschland
    • Biologielaborantin
      • Sep 2015 - Jun 2016

      Analysis and identification of enzymes and proteins with the help of different chromatography and western blots, immunohistochemistry and cloning which includes also q-PCR, PCR, DNA- and RNA-isolation, cDNA synthesis, FPLC, SDS-PAGE, ELISA; Isolation and cultivation of luteal cells, culture of E.coli cells, ligation, transformation, plasmid preparation Analysis and identification of enzymes and proteins with the help of different chromatography and western blots, immunohistochemistry and cloning which includes also q-PCR, PCR, DNA- and RNA-isolation, cDNA synthesis, FPLC, SDS-PAGE, ELISA; Isolation and cultivation of luteal cells, culture of E.coli cells, ligation, transformation, plasmid preparation

    • Praktikum
      • Aug 2015 - Sep 2015

      Chimpanzee tracking, collection of fecal samples, analyses and identification of parasites, field laboratory management, snare patrolling, treatment of domestic animals Chimpanzee tracking, collection of fecal samples, analyses and identification of parasites, field laboratory management, snare patrolling, treatment of domestic animals

    • Government Administration
    • 1 - 100 Employee
    • Praktikum
      • Aug 2014 - Oct 2014

      Identification of Plasmodium parasites in Anopheles darlingi, detection of insecticide resistance in Aedes aegypti and collection of Hylesia metabus (light trapping) and mosquitos (human traps) Identification of Plasmodium parasites in Anopheles darlingi, detection of insecticide resistance in Aedes aegypti and collection of Hylesia metabus (light trapping) and mosquitos (human traps)

    • Germany
    • Hospitals and Health Care
    • 700 & Above Employee
    • Praktikum
      • Jul 2010 - Jul 2010

      Human Pathologie Human Pathologie

Education

  • Lise-Meitner-Schule
    Ausbildung, Biologielaborant
    2011 - 2014

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