Sandra Augustin
Technische Assistentin at Humboldt-Universität zu Berlin- Claim this Profile
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Bio
Experience
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Humboldt-Universität zu Berlin
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Germany
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Higher Education
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700 & Above Employee
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Technische Assistentin
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Sep 2018 - Present
cultivation of stable cell lines (HEK-293, ND7/23) and their preparation and performance of electrophysiological experiments (transfection, patch-clamp), cloning (e.g. Quick-change, Gibson, Transformation); protein purification (HEK-T cells) cultivation of stable cell lines (HEK-293, ND7/23) and their preparation and performance of electrophysiological experiments (transfection, patch-clamp), cloning (e.g. Quick-change, Gibson, Transformation); protein purification (HEK-T cells)
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Max Planck Institute for Infection Biology
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Research Services
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1 - 100 Employee
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Biologielaborantin
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Aug 2016 - Jul 2018
cultivation of pathogenic bacteria, cloning procedure including mutant construction, expression and purification of proteins, biochemical assays, protein and RNA-isolation, preparation of samples for massspectronomy and next-generation sequencing cultivation of pathogenic bacteria, cloning procedure including mutant construction, expression and purification of proteins, biochemical assays, protein and RNA-isolation, preparation of samples for massspectronomy and next-generation sequencing
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Leibniz Institut für Zoo und Wildtierforschung
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Berlin und Umgebung, Deutschland
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Biologielaborantin
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Sep 2015 - Jun 2016
Analysis and identification of enzymes and proteins with the help of different chromatography and western blots, immunohistochemistry and cloning which includes also q-PCR, PCR, DNA- and RNA-isolation, cDNA synthesis, FPLC, SDS-PAGE, ELISA; Isolation and cultivation of luteal cells, culture of E.coli cells, ligation, transformation, plasmid preparation Analysis and identification of enzymes and proteins with the help of different chromatography and western blots, immunohistochemistry and cloning which includes also q-PCR, PCR, DNA- and RNA-isolation, cDNA synthesis, FPLC, SDS-PAGE, ELISA; Isolation and cultivation of luteal cells, culture of E.coli cells, ligation, transformation, plasmid preparation
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Praktikum
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Aug 2015 - Sep 2015
Chimpanzee tracking, collection of fecal samples, analyses and identification of parasites, field laboratory management, snare patrolling, treatment of domestic animals Chimpanzee tracking, collection of fecal samples, analyses and identification of parasites, field laboratory management, snare patrolling, treatment of domestic animals
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Pasteur Institute
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Government Administration
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1 - 100 Employee
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Praktikum
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Aug 2014 - Oct 2014
Identification of Plasmodium parasites in Anopheles darlingi, detection of insecticide resistance in Aedes aegypti and collection of Hylesia metabus (light trapping) and mosquitos (human traps) Identification of Plasmodium parasites in Anopheles darlingi, detection of insecticide resistance in Aedes aegypti and collection of Hylesia metabus (light trapping) and mosquitos (human traps)
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Charité - Universitätsmedizin Berlin
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Germany
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Hospitals and Health Care
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700 & Above Employee
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Praktikum
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Jul 2010 - Jul 2010
Human Pathologie Human Pathologie
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Education
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Lise-Meitner-Schule
Ausbildung, Biologielaborant