Experience

University of Pennsylvania School of Medicine

• Higher Education
• 700 & Above Employee

• Technical Manager

  • Aug 2010 - Present

  I am responsible for the overall technical operation of the core facility, which entails supervision of personnel, ordering of supplies, revision and development of protocols. To this end I am supervising or am personally responsible for the production of reagents, the culture of various cell lines as seed cultures for Penn’s scientists, Endotoxin test (LAL), Mycoplasma test, immunization of mice and rabbits, production (fusion) and screening of hybridomas, production and purification of monoclonal antibodies, transfection of mammalian cells for protein production and selection of stable cell lines.



Integral Molecular

• Production Associate

  • Dec 2009 - Aug 2010

  The main products of the company are lipoparticles for the expression of functionally intact membrane proteins and reporter virus particles (RVP). In addition the company offers services such as protein interaction characterization by Shotgun Mutagenesis and Biosensor analysis. My responsibilities included production and purification of lipoparticles as well as quality control by ELISA and Western. My main responsibility was the development and coordination of immunization schedules with lipoparticles and the characterization of the resulting immune response by ELISA and FACS.



Fox Chase Cancer Center

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Technical Specialist

  • Mar 2009 - Nov 2009

  The focus of the lab was on the analysis of structure and function of the interaction between CFTR and NHERF. My responsibility was expression of proteins in insect cells and chromatographic purification, as well as analysis by SDS-PAGE and Western blot. The focus of the lab was on the analysis of structure and function of the interaction between CFTR and NHERF. My responsibility was expression of proteins in insect cells and chromatographic purification, as well as analysis by SDS-PAGE and Western blot.



Drexel University College of Medicine

• United States
• Higher Education
• 700 & Above Employee

• Lab Manager

  • Jul 2004 - Sep 2008

  The research focus of the lab was HIV/AIDS and developing antagonists of the interaction between HIV-1 envelope protein gp120 and its receptors, CD4 and CCR5. A second research focus was asthma and related inflammatory processes. I was responsible for the operation of the protein core, which entailed recombinant protein expression. To this end I utilized several hollow fiber bioreactors for growth of recombinant mammalian and insect cells, as well as roller bottles and shaker flasks. I was responsible for process development and downstream protein purification by chromatography (FPLC) and validation by ELISA, SDS-PAGE, Western blot and SPR analysis. I was responsible for the analysis of serum from guinea pigs immunized with HIV-1 envelope protein mixed with antagonists. For this I developed several direct binding and inhibition ELISA assays. I instructed and supervised students and personnel.



University of Pennsylvania School of Medicine

• Higher Education
• 700 & Above Employee

• Research Specialist C

  • Jun 1990 - Jul 2004

  The main focus of the work was production (fusion) and cloning of hybridomas and production, purification (FPLC) and subsequent testing of monoclonal antibodies, as well as culturing different types of cells as a service. I did EBV-transformation of human lymphocytes and worked with mouse embryonic stem cells. I was using hollow fiber bioreactors and a minibioreactor to optimize conditions for maximum antibody production. In addition I helped setting up the DNA Sequencing Core Facility and running an automated DNA sequencing service. The work included assembly of sequences, homology searches, Blast searches, ORF analysis and general advice. In both facilities I was responsible for the supervision and training of personnel.