Experience

Verily

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Research Associate

  • Jul 2020 - Present

  South San Francisco, California, United States • Perform high dimensional flow cytometry in support of an immune mapping technology platform. One that aims to identify predictive markers for disease outcome and potential targets for therapeutic intervention • Sort PBMCs using a BD FACSAria for downstream molecular and computational analysis (ATAC-seq/RNA-seq/Immuno-PCR)



Advanced Clinical

• United States
• Biotechnology Research
• 700 & Above Employee

• Laboratory Technologist

  • Jul 2018 - Jul 2020

  San Francisco Bay Area •Supported efforts to scale-up an immune mapping technology platform. One that uses flow cytometry to sort Leukocyte subsets for downstream molecular testing (ATAC-seq/RNA-seq) and computational analysis •Assisted with the first project launch using this technology •Operated Tecan’s laboratory automated workstation called Fluent for use in reagent preparation •Helped with flow analysis using FlowJo analytics software • Processed clinical samples according to established… Show more •Supported efforts to scale-up an immune mapping technology platform. One that uses flow cytometry to sort Leukocyte subsets for downstream molecular testing (ATAC-seq/RNA-seq) and computational analysis •Assisted with the first project launch using this technology •Operated Tecan’s laboratory automated workstation called Fluent for use in reagent preparation •Helped with flow analysis using FlowJo analytics software • Processed clinical samples according to established protocols • Coordinated production with other researchers and maintained accurate sample tracking in a high throughput team environment •Provided feedback to team members about ways to improve efficiency and quality of results •Shared responsibility for providing guidance and support to new team members Show less



HematoLogics Inc.

• United States
• Medical Practices
• 1 - 100 Employee

• Clinical Research Associate

  • 2009 - 2018

  Greater Seattle Area • Maintained the operation and quality control of flow cytometers • Monitored instrument performance and generated reports using excel and some visual basic • Managed the inventory of reagents and supplies • Accessioned patient samples and determined pre-designed panel selection for flow cytometric testing based on clinical history • Trained other members of the team to ensure their understanding of procedure and theory • Sorted cells from peripheral blood and bone marrow… Show more • Maintained the operation and quality control of flow cytometers • Monitored instrument performance and generated reports using excel and some visual basic • Managed the inventory of reagents and supplies • Accessioned patient samples and determined pre-designed panel selection for flow cytometric testing based on clinical history • Trained other members of the team to ensure their understanding of procedure and theory • Sorted cells from peripheral blood and bone marrow aspirate specimens for chimerism studies using a BD FACSAria cell sorter at the Fred Hutchinson Cancer Research Center • Processed patient specimens with the purpose of screening for Leukemia and Lymphoma • Reviewed patient reports before they were submitted to the client or the ordering physician while checking for accuracy and making corrections • Helped compile and graph data points for a submission to the FDA • Conducted validation experiments and wrote internal documents for use in establishing records for CAP inspections and laboratory accreditation standards • Performed testing on Acute Myeloid Leukemia samples for studies which aimed to correlate minimal residual disease detection with patient treatment, prognostic outcome, and survival statistics • Prepared and validated PCR master mixes for molecular testing • Performed DNA isolations for downstream molecular testing Show less



Cascade Subscription Service, Inc.

• United States
• Book and Periodical Publishing
• 1 - 100 Employee

• Administrative Assistant

  • 2005 - 2009

  Greater Seattle Area • Maintained customer accounts • Investigated and resolved customer service inquiries • Provided basic in-house technology support • Designed and managed mail marketing campaigns



Corixa Corporation

• United States
• Biotechnology
• 1 - 100 Employee

• Research Associate I - Process Science Department

  • 2003 - 2004

  Greater Seattle Area • Helped develop and optimize scalable purification methods for protein antigens • Purified antibodies using affinity columns • Assisted with writing of internal development reports

• Research Associate I - Monoclonal Anitbody Development Group

  • 2002 - 2003

  Greater Seattle Area • Involved with construction of mammalian expression constructs for both human and mouse expression (i.e. pCEP4, pBROAD, pBUDCE4.1) • Validated cell surface expression for all assigned monoclonal antibody targets • Helped generate stable lines for select monoclonal antibody targets based on their initial characterization • Assisted in the generation of hybridomas for the purpose of downstream monoclonal antibody development and screening

• Research Associate I - (Hiring Manager and Supervisor) – Antigen Discovery

  • 2000 - 2002

  Greater Seattle Area • Supervised and coordinated the efforts of part time employees whose purpose was to complete the preparation and organization of templates used for Microarray. • Consolidated bacterial cell cultures into 96-well plates based on PCR amplification results • Assisted with the launch and continued operation of Microarray in-house. • Helped design and perform experiments with the purpose of optimizing the Microarray process. • Maintained and improved a Microarray database that… Show more • Supervised and coordinated the efforts of part time employees whose purpose was to complete the preparation and organization of templates used for Microarray. • Consolidated bacterial cell cultures into 96-well plates based on PCR amplification results • Assisted with the launch and continued operation of Microarray in-house. • Helped design and perform experiments with the purpose of optimizing the Microarray process. • Maintained and improved a Microarray database that integrated clone tracking and Microarray quality control using descriptive statistics. • Responsible for the installation and operation of a Qiagen Biorobot 8000 with the purpose of automating the Microarray target preparation process. • Assisted other researchers with the use of GeneSpring clustering software in the analysis of their Microarray data. • Coordinated sequencing efforts for IBD (Inflammatory Bowel Disease) and Darpa (Defense Advanced Research Projects Agency) projects.

• Part Time Laboratory Assistant – Antigen Discovery

  • 1998 - 2000

  Greater Seattle Area • Completed the preparation and organization of cDNA clones used for Microarray • Assisted researchers with other antigen discovery projects when needed