Scientist

• Sep 2021 - Present

Associate Scientist

• Aug 2020 - Sep 2021

Senior Research Associate

• Feb 2019 - Aug 2020

Associate Process Engineer - Purification

• Sep 2015 - Feb 2019

-Technical support for purification operations of multiple products -Process development / optimization / continuous improvement -DoE for process optimization -AKTA systems -R/D cross function -TFF -Automated method development - Analytical Assays: pNP Enzyme Kinetics HCP ELISA SDS PAGE Department HSE (Health, Safety, and Environment) Representative -Technical support for purification operations of multiple products -Process development / optimization / continuous improvement -DoE for process optimization -AKTA systems -R/D cross function -TFF -Automated method development - Analytical Assays: pNP Enzyme Kinetics HCP ELISA SDS PAGE Department HSE (Health, Safety, and Environment) Representative

Research Laboratory Technician

• Sep 2013 - Jun 2014

Performed research on abbreviated alpha-1-antitrypsin (A1AT). This protein acts as a protease inhibitor in its native state, which prevents various proteins in the cell from being degraded. When in a functionally unfavorable conformation, A1AT is prone to aggregation in cells and can cause a variety of symptoms. I routinely prepared plasmid transformations and cell cultures of E.coli, lysed cells, washed pellets, and purified abbreviated A1AT in its soluble and insoluble conformations… Show more Performed research on abbreviated alpha-1-antitrypsin (A1AT). This protein acts as a protease inhibitor in its native state, which prevents various proteins in the cell from being degraded. When in a functionally unfavorable conformation, A1AT is prone to aggregation in cells and can cause a variety of symptoms. I routinely prepared plasmid transformations and cell cultures of E.coli, lysed cells, washed pellets, and purified abbreviated A1AT in its soluble and insoluble conformations. I often prepared and analyzed SDS-PAGE assays with the goal of process optimization. A more explicit list of skills includes: competent cell prep, molecular cloning from E.coli to S. cervisiae, protein expression via the lac-operon with IPTG as an inducer, bacterial selection with various antibiotics, spectrophotometry, IMAC, SEC, and HIC column packing and usage, distillation, medium prep, Bradford Assay, PCR, primer selection, sterile culture inoculation, autoclave sterilization, agar plate preparation, centrifugation, microscopy, micro pipetting, mini-prep procedures, plasmid transformation, incubation, sonication, microfluidization, extraction using organic solvents, simple/fractional distillation, and recrystallization. Show less Performed research on abbreviated alpha-1-antitrypsin (A1AT). This protein acts as a protease inhibitor in its native state, which prevents various proteins in the cell from being degraded. When in a functionally unfavorable conformation, A1AT is prone to aggregation in cells and can cause a variety of symptoms. I routinely prepared plasmid transformations and cell cultures of E.coli, lysed cells, washed pellets, and purified abbreviated A1AT in its soluble and insoluble conformations… Show more Performed research on abbreviated alpha-1-antitrypsin (A1AT). This protein acts as a protease inhibitor in its native state, which prevents various proteins in the cell from being degraded. When in a functionally unfavorable conformation, A1AT is prone to aggregation in cells and can cause a variety of symptoms. I routinely prepared plasmid transformations and cell cultures of E.coli, lysed cells, washed pellets, and purified abbreviated A1AT in its soluble and insoluble conformations. I often prepared and analyzed SDS-PAGE assays with the goal of process optimization. A more explicit list of skills includes: competent cell prep, molecular cloning from E.coli to S. cervisiae, protein expression via the lac-operon with IPTG as an inducer, bacterial selection with various antibiotics, spectrophotometry, IMAC, SEC, and HIC column packing and usage, distillation, medium prep, Bradford Assay, PCR, primer selection, sterile culture inoculation, autoclave sterilization, agar plate preparation, centrifugation, microscopy, micro pipetting, mini-prep procedures, plasmid transformation, incubation, sonication, microfluidization, extraction using organic solvents, simple/fractional distillation, and recrystallization. Show less

Lifeguard

• May 2012 - Sep 2013

Led a team of lifeguards in pool deck supervision, pool deck security, lane line manipulation, and safety enforcement without administrative supervision. Led a team of lifeguards in pool deck supervision, pool deck security, lane line manipulation, and safety enforcement without administrative supervision.