Experience

MDI Biological Laboratory

• Assistant Professor

  • Dec 2020 - Present

  Kathryn W. Davis Center for Regenerative Biology and Aging



Stanford University

• United States
• Higher Education
• 700 & Above Employee

• Life Sciences Research Associate, Ph. D. Molecular Biology / Neurobiology

  • Oct 2011 - Present

  United States I am investigating the function single nucleotide polymorphisms associated to human diseases. A computational screen led us to identify human regulatory SNPs conserved across vertebrates and involved in a large variety of diseases, including for example psychiatric disorders, brain vasculature defects or brain cancer. The identification of the target gene affected by the SNP allows me to study the function of this gene in the biological process associated to the human disorder. Recently, this… Show more I am investigating the function single nucleotide polymorphisms associated to human diseases. A computational screen led us to identify human regulatory SNPs conserved across vertebrates and involved in a large variety of diseases, including for example psychiatric disorders, brain vasculature defects or brain cancer. The identification of the target gene affected by the SNP allows me to study the function of this gene in the biological process associated to the human disorder. Recently, this project led me to the identification of transcription factors coupling neurogenesis and angiogenesis in the human central nervous system, and I am currently studying their functions in the neuronal regeneration process using zebrafish and mouse as integrated model systems or human neural stem cells for in vitro assays. My current work also takes the challenge to reprogram endogenous neural stem cells to allow the neuronal regeneration to restore vision in eye diseases like age-related macular degeneration. Contrary to the mouse retina, the zebrafish retina has the capacity to fully regenerate all the neuronal subtypes after injury. This process is dependent of the Müller cells, the neural stem cells of the retina. While there is still a long way to go, our objective is to identify instructive factors for retinal regeneration in mammals. Show less



RESTORE

• United Kingdom
• Construction

• Research Associate, Regenerative Biology

  • Dec 2018 - Apr 2021

  Toulouse



Center of Developmental Biology (CBD), CNRS UMR5547

• PhD Graduate Student

  • Sep 2007 - Jul 2011

  Toulouse Area, France Identification of new target genes of the Ngn1 proneural factor and analysis of their functions in neuronal determination and specification in zebrafish. Supervisors: Dr. P. Blader (e-mail: patrick.blader@univ-tlse3.fr). - Gained extensive knowledge in the genetic of the developmental neurobiology. - Identified new target genes of the proneural transcription factor Neurogenin1 and studied their functions in in the control of the cell behavior. - Performed biochemical experiments to… Show more Identification of new target genes of the Ngn1 proneural factor and analysis of their functions in neuronal determination and specification in zebrafish. Supervisors: Dr. P. Blader (e-mail: patrick.blader@univ-tlse3.fr). - Gained extensive knowledge in the genetic of the developmental neurobiology. - Identified new target genes of the proneural transcription factor Neurogenin1 and studied their functions in in the control of the cell behavior. - Performed biochemical experiments to validate Neurogenin1 target genes (Band-Shift and ChIP). - Performed in vivo gain and loss of function experiments to study genes function. - Performed in vivo time lapse imaging to study the cellular behavior (mophogenesis and axogenesis). - Published 4 articles in peer reviewed journals - Organized student symposia inviting keynote speakers as a member of the Ph.D Student Association (Alpha T) Show less

• Master Student Research Assistant

  • Sep 2006 - Jun 2007

  Toulouse Area, France Divergent transcriptional activity of proneural genes of the atonal (ngn1) and achaete-scute (ascl1a) family in zebrafish. Supervisor: Dr. P. Blader (e-mail: patrick.blader@univ-tlse3.fr). - Gained knowledge in transcriptional controls of the neurogenesis. - Gained experience in molecular biology (Cat/bGal ELISA assay, ISH and ChIP), zebrafish biology (Establishment of transgenic lines, Microinjection of MO and mRNA and use of pharmacological agents on embryos) and confocal… Show more Divergent transcriptional activity of proneural genes of the atonal (ngn1) and achaete-scute (ascl1a) family in zebrafish. Supervisor: Dr. P. Blader (e-mail: patrick.blader@univ-tlse3.fr). - Gained knowledge in transcriptional controls of the neurogenesis. - Gained experience in molecular biology (Cat/bGal ELISA assay, ISH and ChIP), zebrafish biology (Establishment of transgenic lines, Microinjection of MO and mRNA and use of pharmacological agents on embryos) and confocal microscopy. - Carried out experimental work Show less



Molecular and Cellular Interactions, CNRS UMR6026

• Bachelor Student Research Assistant

  • Jan 2006 - Jun 2006

  Rennes Area, France Function of the HSP70 chaperon in the translational regulation and subcellular localization of SCYTHE. Supervisor: Dr. D. Michel (e-mail: denis.michel@univ-rennes1.fr). - Gained knowledge in transcriptional and translational mechanisms. - Gained experience in molecular biology (vector design, cloning and PCR) and cell culture assays (Immunostaining, Immunoprecipitation and Western blot). - Carried out experimental work