Experience

Axolotl Biologix

• United States
• Biotechnology
• 1 - 100 Employee

• Senior Research Scientist

  • Dec 2019 - Present

  Flagstaff, AZ

• Laboratory Manager, Research Scientist

  • Nov 2017 - Dec 2019

  Flagstaff, AZ 86001



Finding Your Gold

• Small Business Owner

  • Feb 2011 - Present

  Life and wellness coach specializing in life transitions for men in later years or at any adult age when a notable life transition enters one's life, fundamentally changing career, relationships, life plan, sense of purpose, etc.



Northern Arizona University

• United States
• Higher Education
• 700 & Above Employee

• Laboratory Manager

  • Nov 2017 - Mar 2021

  Flagstaff, Arizona Train, support and manage students, including undergraduates, post baccalaureate interns, MS and PhD students.



University of Massachusetts Lowell

• United States
• Higher Education
• 700 & Above Employee

• Research Assistant

  • Jul 2017 - Oct 2017

  Lowell, MA Protein purification projects on the muscle protein titin in collaboration with Northern Arizona University. Work included bacterial transformation and recombinant protein expression, protein purification, chromatography (IMAC, IEX, SEC, FPLC), electrophoresis, circular dichroism, protein folding, intrinsically disordered proteins.

• Lab Manager, Technical Researcher

  • Apr 2015 - Sep 2016

  Lowell, MA Perform individual and collaborative research projects on the muscle protein titin, an intrinsically disordered protein. Work includes bacterial transformation and recombinant protein expression, protein and peptide purification, chromatography (LC, FPLC, HPLC, LC/MS), electrophoresis. Chemical hygiene and safety officer.

• Manager, Biomolecular Characterization Laboratory

  • May 2015 - Feb 2016

  Lowell, MA Part-time work as staff and lab manager setting up a new Biomolecular Characterization Laboratory (BCL) within the Core Research Facilities Department on the UMass Lowell campus. Work included setting up policies, procedures, instrument training, staff hiring and training. Equipment in the BCL included Illumina NextSeq 500 Next Generation Sequencer, Shimadzu 8040 Triple Quadrupole LC/MS, Amnis FlowSight imaging flow cytometer, Bio-Rad XPR Surface Plasmon Resonance, Waters Gel Permeation… Show more Part-time work as staff and lab manager setting up a new Biomolecular Characterization Laboratory (BCL) within the Core Research Facilities Department on the UMass Lowell campus. Work included setting up policies, procedures, instrument training, staff hiring and training. Equipment in the BCL included Illumina NextSeq 500 Next Generation Sequencer, Shimadzu 8040 Triple Quadrupole LC/MS, Amnis FlowSight imaging flow cytometer, Bio-Rad XPR Surface Plasmon Resonance, Waters Gel Permeation Chromatography (GPC) system, Bio-Rad Experion Automated Electrophoresis Station, Qubit DNA/RNA fluorometer, Bio-Rad CFX Connect 96-well qPCR and Bio-Rad S1000 96-well thermal cycler.



Development Engineering Sciences, LLC

• United States

• Researcher & Lab Manager

  • Oct 2010 - Oct 2015

  Flagstaff, AZ Senior scientist at contract research organization, performing a wide range of research: product testing, validation, biocompatibility testing, cell culture, viability testing, bench-top failure testing, enzyme and chemical assays. Train undergraduate and graduate students. Establish safety procedures and regulatory compliance contracts. Train, support and manage student interns from Northern Arizona University, including undergraduates, post baccalaureate interns, MS and PhD students.



Protein Genomics, Inc

• Lab Manager

  • Feb 2010 - Dec 2014

  Flagstaff, AZ Setup new R&D laboratory. Perform a wide array of lab experiments in the biomedical arena, including in vitro biocompatibility testing, cell culture (primary fibroblasts, endothelial cells, smooth muscle cells, and adipose-derived stem cells), enzyme assays, etc. Train undergraduate and graduate students. Establish safety procedures and regulatory compliance contracts.

• Lab Manager

  • Feb 2005 - Feb 2010

  Flagstaff, AZ Lab Manager for the Discovery Research Laboratories (2/05-1/09) and for Center for Applied Research in Environmental Endocrinology (1/09-2/10) with finding from grants and SenesTech.



SenesTech, Inc.

• United States
• Chemical Manufacturing
• 1 - 100 Employee

• Lab Manager

  • Aug 2008 - Dec 2009

  Flagstaff, AZ Employed as Lab Manager

• Consulting Researcher and Lab Manager

  • Feb 2005 - Aug 2008

  Flagstaff, AZ



Coconino County Health Department

• United States
• Government Administration
• 1 - 100 Employee

• Program Coordinator, HIV Outreach and Prevention

  • Jan 2001 - Dec 2004

  Flagstaff, AZ Coordinate HIV prevention program targeting high risk populations in Coconino County, develop, operate and recruit community members for Tri-County Men’s Health program, perform HIV counseling and testing, train new HIV counselors following CDC and Arizona Department of Health Services guidelines

• Research Specialist

  • Sep 1998 - Dec 2000

  Flagstaff, AZ Mammalian studies: Ovarian theca-interstitial cell regulation, ApoE isoform studies, apoptosis, mammalian cell culture. Rana catesbeiana: molecular work including nucleic acid isolation and cloning



Western Newspapers, Inc.

• United States

• Graphic Ad Designer

  • Feb 1997 - Sep 1998

  Flagstaff, AZ First position after relocating to Flagstaff. Crafted ads for Canyon Shopper (weekly ad publication) and Navajo Hopi Observer (weekly newspaper), preflight check, managed and printed postcard mailer



Avante International, Inc.

• United States
• Automation Machinery Manufacturing
• 1 - 100 Employee

• Vice President and Co-founder

  • Mar 1993 - Feb 1998

  Madison, WI and Flagstaff, AZ 501(c)(3) educational organization created to raise public awareness about HIV/AIDS. Prepare plaster facial cast of individuals, build and display a team-built multimedia exhibit, graphic design or promotional materials, prepare 501(c)(3) application, budget, etc.



University of Wisconsin Hospital and Clinics

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Research Specialist

  • Sep 1993 - Dec 1996

  Madison, WI Develop large scale method for extracting and purifying fungal surface antigen of Blastomyces dermatitidis, mammalian cell culture, macrophage and T-cell purification, EBV transformation of T-cells and cloning, bacterial expression and purification of cloned genes and fragments, flow cytometry. Two publications.



Emerald Rose Bookstore

• Assistant Manager

  • Aug 1989 - Nov 1992

  Boulder, CO Sales, computer inventory/database, merchandizing, scheduling employees, scheduling practitioners, editing newsletter



Worcester Foundation Campus of the University of Massachusetts Medical Center

• Research Specialist

  • Jun 1984 - Jul 1986

  Shrewsbury, MA Determine transcriptional and translational control in immature and mature Xenopus laevis oocytes, microinject all stages of oocytes, purify and assay smooth and rough endoplasmic reticulum, immunize rabbits, collect and purify polyclonal antibodies, protein radioiodination. DNA/RNA isolation and analysis via Northern And Southern blots



Harvard Medical School

• United States
• Higher Education
• 700 & Above Employee

• Research Technician

  • Jun 1983 - Jun 1984

  Boston, MA Scale up existing methods for purifying Protein Kinase Types I and II from rat and bovine skeletal and cardiac muscle. Immunize guinea pigs and rabbits. Generate and purify polyclonal antibodies



University of Massachusetts Amherst

• United States
• Higher Education
• 700 & Above Employee

• Undergraduate Student Researcher

  • Sep 1982 - May 1983

  Amherst, MA Large scale culture of Aspergillus species for extraction and digestion of fungal wall glucan nigeran and purification of oligosaccharides for sale to Sigma. Performed pulse-chase 14C-labeling to study nigeran biosynthetic pathways.