Robert Apilado
Senior Scientist, Flow Cytometry at Invivoscribe- Claim this Profile
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Bio
Experience
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Invivoscribe
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United States
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Biotechnology Research
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100 - 200 Employee
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Senior Scientist, Flow Cytometry
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Jan 2020 - Present
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Fate Therapeutics Inc
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United States
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Biotechnology Research
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200 - 300 Employee
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Associate Scientist
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Jan 2019 - Jan 2020
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Exagen Inc.
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United States
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Biotechnology Research
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100 - 200 Employee
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Flow Cytometry Scientist
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Jan 2018 - Jan 2019
• Clinical and analytical validation of a flow cytometry assay to measure Cell Bound Complement Activation Products(CB-CAPs) on platelets, PC4d • Transfer of laboratory developed PC4d assay to the Clinical Laboratory • Development of a multi-parameter flow cytometry panel to measure Cell Bound Complement Activation Products on B, T, M and NK cell sub-populations
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Assay Development and Technology Transfer Scientist
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Feb 2017 - Jan 2018
• Research, develop, and optimize quantitative clinical assays • Validation of laboratory developed clinical assays • Create detailed Standard Operating Procedures for use in the Clinical Laboratory • Directly assist in the transfer of laboratory developed assays from Research and Development to the Clinical Laboratory • Experienced in: LC-MS/MS, HPLC, Flow Cytometry, and various protein assays
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Clinical Laboratory Technician
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Mar 2013 - Jan 2017
•Operation of Thermofisher TS Quantiva Mass Spectrometer. •Therapeutic drug level monitoring of Methotrexate and Hydroxychloroquine levels using an LC-MS/MS tandem system. • Operation and maintenance of multiple INOVA Bio-Flash instruments for the analysis of several markers by chemiluminescent assay • Operation of multiple Thermo Scientific Phadia 250 Systems for both ImmunoCAP and ELiA technologies • Operation of Beckman Coulter FC 500 and Gallios systems to determine C4d levels… Show more •Operation of Thermofisher TS Quantiva Mass Spectrometer. •Therapeutic drug level monitoring of Methotrexate and Hydroxychloroquine levels using an LC-MS/MS tandem system. • Operation and maintenance of multiple INOVA Bio-Flash instruments for the analysis of several markers by chemiluminescent assay • Operation of multiple Thermo Scientific Phadia 250 Systems for both ImmunoCAP and ELiA technologies • Operation of Beckman Coulter FC 500 and Gallios systems to determine C4d levels on erythrocytes and B lymphocytes • Ordering reagents and maintaining inventory • Qualifying new reagent lots • Performing instrument comparisons • Establishing and updating SOPs for various equipment and assays
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Genoptix, Inc.
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United States
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Hospitals and Health Care
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1 - 100 Employee
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Clinical Laboratory Technician II
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2009 - 2013
• Process blood, bone marrow, and solid tissue for immunophenotyping of leukemia and lymphoma in a high volume CLIA registered and CAP certified laboratory, processing 200 samples a day • Operation and maintenance of BD FACSANTO II • Analyze flow cytometry data using BD FACSDiva Software and FCS Express • Use of LIS to perform editing, data entry, quality control and quality assurance • Attended minimum of 12 Genoptix approved CEU per year • Train new hire technicians as needed • Process blood, bone marrow, and solid tissue for immunophenotyping of leukemia and lymphoma in a high volume CLIA registered and CAP certified laboratory, processing 200 samples a day • Operation and maintenance of BD FACSANTO II • Analyze flow cytometry data using BD FACSDiva Software and FCS Express • Use of LIS to perform editing, data entry, quality control and quality assurance • Attended minimum of 12 Genoptix approved CEU per year • Train new hire technicians as needed
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Vet-Stem, Inc
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Poway, CA
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Laboratory Technician
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2008 - 2009
• Accurately and timely accession patient samples and test requisitions • Isolation of adipose derived stem cells in a sterile production environment for use in autologous treatment of animals • Cryopreserving extra doses for future use • Accurately and timely accession patient samples and test requisitions • Isolation of adipose derived stem cells in a sterile production environment for use in autologous treatment of animals • Cryopreserving extra doses for future use
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eBioscience
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United States
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Biotechnology Research
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1 - 100 Employee
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Laboratory Technician
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2003 - 2008
• Test various antibodies and reagents by Western Blot and Flow Cytometry using FACSCalibur, FACSSort, Cell Quest • Prepare mouse, rat, and human cells for immune staining • Assist in tissue culture • Handle and dispose of biohazardous materials • Test various antibodies and reagents by Western Blot and Flow Cytometry using FACSCalibur, FACSSort, Cell Quest • Prepare mouse, rat, and human cells for immune staining • Assist in tissue culture • Handle and dispose of biohazardous materials
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Education
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San Diego State University-California State University
Bachelor of Science (BS), Biology