Experience

Clevergene

• India
• Biotechnology Research
• 1 - 100 Employee

• Senior Research Associate

  • Apr 2021 - Feb 2023

  Bengaluru, Karnataka, India NGS, NGS library preparation (Illumina Sequencing platform, Oxford Nanopore)



Qtlomics

• India
• Research Services
• 1 - 100 Employee

• Research Associate

  • Sep 2019 - Apr 2021

  Bangalore Urban, Karnataka, India Nanopore Sequencing, Nanopore library prep, Mol bio (Oxford Nanopore platform, Illumina Sequencing Platform)



MEDGENOME

• United States
• Biotechnology Research
• 700 & Above Employee

• Research Associate

  • May 2017 - Aug 2019

  Bangalore Working on Pre-implantation Genetic Screening (Illumina Sequencing Platform)



Molecular Connections

• India
• Information Services
• 700 & Above Employee

• Research Analyst

  • May 2016 - Mar 2017

  Bangalore



Pest Control India (Pvt) Ltd

• India
• Consumer Services
• 500 - 600 Employee

• Technical Officer

  • May 2013 - Apr 2016

  bangalore My work was on insect behavioral studies by single tube, Y-tube and wind tunnel olfactrometer, collection of plant and insects volatile, ensuring quality compliance and quality control of different products before marketing, conducting field trial of different products and preparing data, statistical analysis of field data by different statistical tools like ANOVA, t-Test



Kidwai Memorial Institute of Oncology - Company

• Higher Education
• 1 - 100 Employee

• Senior Research Fellow

  • Dec 2012 - May 2013

  bangalore Worked on membrane fatty acid analysisof RBC for different cancer and normal age matched women. My work mainly involved isolation of red blood cells from whole blood and then membrane fatty acid isolation by Bligh and Dyer methodby Gas Chromatography. Isolation of immunecells by flowcytrometry Isolation of RBC from whole blood by density gradient centrifugation Isolation of lymphocytes,mononucleargranulocytes, macrophages by Ficoll Paque density gradient method. Isolation of membranefatty acid… Show more Worked on membrane fatty acid analysisof RBC for different cancer and normal age matched women. My work mainly involved isolation of red blood cells from whole blood and then membrane fatty acid isolation by Bligh and Dyer methodby Gas Chromatography. Isolation of immunecells by flowcytrometry Isolation of RBC from whole blood by density gradient centrifugation Isolation of lymphocytes,mononucleargranulocytes, macrophages by Ficoll Paque density gradient method. Isolation of membranefatty acid by Bligh and Dyer method Analysis of membranefatty acid by Gas-Chromatography. Experiment designing, report validation and data analysis. Validation studies (stability, imprecision and equivalence studies) on DuraClone HLA-B27 reagent on BD (FACS, SCAN, FACS-Calibur) flowcytometers. Show less



University of Agricultural Sciences, Bangalore

• Higher Education
• 100 - 200 Employee

• Research Assistant

  • Mar 2008 - Nov 2012

  bangalore Production of Wilt resistant banana by tissue culture method. Fussariumis a common microorganism cause wilt in banana (Nanjanguda rasabare). Production of callus to make the cell lines which is resistant to the infection. Then production of plant culture through suspension culture for the further production of disease resistant plant embryo Production of fussarium resistant plant by Agrobacteriummediated recombinationmethod Different culture media preparation for plant tissue culture Cell… Show more Production of Wilt resistant banana by tissue culture method. Fussariumis a common microorganism cause wilt in banana (Nanjanguda rasabare). Production of callus to make the cell lines which is resistant to the infection. Then production of plant culture through suspension culture for the further production of disease resistant plant embryo Production of fussarium resistant plant by Agrobacteriummediated recombinationmethod Different culture media preparation for plant tissue culture Cell suspension culture for the production of disease resistant plant Studies and analysis of genetic diversity of different banana species by Amplified Fragment Length Polymorphism(AFLP) method Comparative studies of genetic diversity between wild species of banana and Nanjanguda rasabare by AFLP and Random Amplified PolymorphicDNA ( RAPD) marker. Quantitative Trait Loci (QTL) studies for diverse plant species. Data analysis, Validation of report, experiment designing. Show less



Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR)

• Higher Education
• 200 - 300 Employee

• Project Assistant

  • Oct 2007 - Mar 2008

  bangalore Production of bacterial clones(E.coli) expressing the protein Dam1 ( a protein find in budding yeast essential for yeast chromosomal segregation) & production of antibody by injecting that protein in mice for the further study about the genomic as well as centromere structure of Candida albicans (a pathogenic yeast shows its negative effect in immunosuppressed patient) and also to find out whether any unknown proteins are present in the Candida kinetochore Isolation of genomic DNA of Yeast… Show more Production of bacterial clones(E.coli) expressing the protein Dam1 ( a protein find in budding yeast essential for yeast chromosomal segregation) & production of antibody by injecting that protein in mice for the further study about the genomic as well as centromere structure of Candida albicans (a pathogenic yeast shows its negative effect in immunosuppressed patient) and also to find out whether any unknown proteins are present in the Candida kinetochore Isolation of genomic DNA of Yeast ,Candida albicans and E.coli Primer designing for PCR reaction. Amplification of DNA by PCR reaction Restriction digestion, ligation of fungal DNA in bacterial cloning vector. Expression of proteins in bacterial expression vector Selection desired proteins by SDS-gel electrophoresisand westernblotting Analysis of protein by Mass Spectrophotometer(MALDI-TOF) Raising the antibody in mice Show less



National Centre for Biological Sciences

• India
• Research Services
• 200 - 300 Employee

• Project Trainee

  • Jul 2007 - Sep 2007

  bangalore Production of recombinant Drosophila melanogaster by genetic crossing to find out the effect of NSF & SNAPprotein factor in mitochondrialdynamics in fruit fly Production of recombinant drosophila by genetic cloning method Identification of mitochondrialdynamics by confocal microscopy