Biology Co-op

• Jan 2023 - Jun 2023

• Culture, maintain, and cryopreserve multiple cancer cell lines using aseptic techniques • Identify the mechanism of action of small molecule inhibitors by analyzing protein expression using Western Blotting, ELISA, and AlphaLISA of various downstream pathway genes • Analyze the effects of inhibitors on cell viability and cell cycle regulation by cell-based functional assays using flowcytometry and Incucyte • Isolate and investigate the m-RNA expression in treated and untreated cell… Show more • Culture, maintain, and cryopreserve multiple cancer cell lines using aseptic techniques • Identify the mechanism of action of small molecule inhibitors by analyzing protein expression using Western Blotting, ELISA, and AlphaLISA of various downstream pathway genes • Analyze the effects of inhibitors on cell viability and cell cycle regulation by cell-based functional assays using flowcytometry and Incucyte • Isolate and investigate the m-RNA expression in treated and untreated cell lines using quantitative PCR • Determine lead inhibitors IC50s in proliferation assays using endpoint assays such as Cell Titer Glo, CyQUANT Direct, CyQUANT MTT and colony formation assays • Use genetic tools like siRNA knockdown and CRISPR/Cas9 knockout studies to understand the effect of key gene silencing on cellular sensitivity and downstream pathway activation • Investigated the regulation of key proteins in cell cycle, senescence, and apoptosis in treated cells using immunofluorescence • Conduct rescue experiments with the CRISPR-Cas9 system and overexpression of protein to evaluate potential off-target effects of inhibitors Show less • Culture, maintain, and cryopreserve multiple cancer cell lines using aseptic techniques • Identify the mechanism of action of small molecule inhibitors by analyzing protein expression using Western Blotting, ELISA, and AlphaLISA of various downstream pathway genes • Analyze the effects of inhibitors on cell viability and cell cycle regulation by cell-based functional assays using flowcytometry and Incucyte • Isolate and investigate the m-RNA expression in treated and untreated cell… Show more • Culture, maintain, and cryopreserve multiple cancer cell lines using aseptic techniques • Identify the mechanism of action of small molecule inhibitors by analyzing protein expression using Western Blotting, ELISA, and AlphaLISA of various downstream pathway genes • Analyze the effects of inhibitors on cell viability and cell cycle regulation by cell-based functional assays using flowcytometry and Incucyte • Isolate and investigate the m-RNA expression in treated and untreated cell lines using quantitative PCR • Determine lead inhibitors IC50s in proliferation assays using endpoint assays such as Cell Titer Glo, CyQUANT Direct, CyQUANT MTT and colony formation assays • Use genetic tools like siRNA knockdown and CRISPR/Cas9 knockout studies to understand the effect of key gene silencing on cellular sensitivity and downstream pathway activation • Investigated the regulation of key proteins in cell cycle, senescence, and apoptosis in treated cells using immunofluorescence • Conduct rescue experiments with the CRISPR-Cas9 system and overexpression of protein to evaluate potential off-target effects of inhibitors Show less