Rebecca (Jones) Mauney

Scientist II at BioAgilytix
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Contact Information
us****@****om
(386) 825-5501
Location
Knightdale, North Carolina, United States, US

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Experience

    • United States
    • Biotechnology Research
    • 700 & Above Employee
    • Scientist II
      • Apr 2023 - Present

    • Scientist I
      • Mar 2021 - Apr 2023

    • United States
    • Research
    • 100 - 200 Employee
    • Research Analyst I
      • Feb 2019 - Mar 2021

      • Independently conducted the isolation of memory B cells from specimens collected from HIV-positive human and non-human primate vaccine recipients by fluorescent-activated single-cell sorting. • Independently performed high-throughput cultures of primary memory B cells and high-throughput functional screenings. • Compiled, analyzed and summarized experimental results in both tabular and graphic forms, and Draw experiment conclusions provided to PI for further discussion. Trained new team members in general laboratory techniques and operation of equipment. • Assisted with, trained and supervised new employees for the laboratory high throughput sample pooling of the Duke MASC Program. • Through troubleshooting, improved performance, and reliability of Memory B Cell culture screening ELISA assay was obtained. • Optimized conditions and performed ELISAs to characterize Y. pestis-specific antibodies from immunized mice. • Achieved high throughput and yield of produced monoclonal antibodies by executing transient transfections, purification and quality control SDS/PAGE and Western blots. • Optimized conditions and performed immunoprecipitations to demonstrate the ability of glycan-reactive HIV-1-specific broadly neutralizing antibodies to cross-react with HLA-DR expressed on human B cells. • Supported laboratory organization by writing and updating SOPs, and supported data provenance goals by keeping highly detailed records of all experiments • Produced accurate budget information in a timely fashion to support grant and contract proposals. • Assisted the PI in preparing figures and methods sections of manuscripts, grants and presentations. • Prepared and presented a poster at a scientific meeting. • Efficiently maintained laboratory supply inventory and managed ordering to ensure sufficient stocks. • Knowledge of operations to numerous automation technology including Biomek liquid handling automation systems, and Biomek plate washers,. Show less

    • United States
    • Biotechnology Research
    • 200 - 300 Employee
    • Product Safety Toxicologist
      • May 2018 - Jul 2019

      • Human health risk assessments. o Data analysis on personal care product compounds and their toxicity to human health. • Environmental risk assessments. o Data analysis on personal care product compounds and their toxicity to the environment. o QSAR, EPISUITE, and ECOSAR. • Human health risk assessments. o Data analysis on personal care product compounds and their toxicity to human health. • Environmental risk assessments. o Data analysis on personal care product compounds and their toxicity to the environment. o QSAR, EPISUITE, and ECOSAR.

    • Master of Science in Toxicology
      • Aug 2016 - Apr 2018

      • Utilized an ovariectomized and hormonally intact mouse model of Endometriosis to assess the effects of BPA and BPAF on the potentiation of the disease. • Examined uterine, lesion, and ovarian characteristics by weight, volume, H&E, IHC, and qPCR. • Analyzed hormone levels in blood plasma by ELISA. • Utilized an ovariectomized and hormonally intact mouse model of Endometriosis to assess the effects of BPA and BPAF on the potentiation of the disease. • Examined uterine, lesion, and ovarian characteristics by weight, volume, H&E, IHC, and qPCR. • Analyzed hormone levels in blood plasma by ELISA.

    • United States
    • Environmental Services
    • 1 - 100 Employee
    • Chemist; Analytical and Organic
      • Jun 2014 - Jul 2016

      IR, GC, GC-MS, Wet Chemistry, TCLP, Semi-Volatile Extractions, Volatile Extractions (ZHE), BTU/Cl, Total Mercury, Cyanide distillation, %alcohol, % glycol, %phenol, UV-vis spectroscopy, 1H- and 13C-NMR. Troubleshooting the GC/GC-MS with instrument repair. As well as Lab Trainer. IR, GC, GC-MS, Wet Chemistry, TCLP, Semi-Volatile Extractions, Volatile Extractions (ZHE), BTU/Cl, Total Mercury, Cyanide distillation, %alcohol, % glycol, %phenol, UV-vis spectroscopy, 1H- and 13C-NMR. Troubleshooting the GC/GC-MS with instrument repair. As well as Lab Trainer.

Education

  • University of Cincinnati College of Medicine
    Master's degree, Toxicology
    2016 - 2018
  • Baldwin Wallace University
    Bachelor of Applied Science (B.A.Sc.), Chemistry
    2010 - 2014

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