• Dec 2022 - Present

• Jun 2018 - Dec 2022

Responsibilities:Managing the project of protein analytics research, including budget control, data analysis, and reporting. Full MS-support for research associates/collaborator MS-projects (conceptualization, realization, data analysis).Design, development, and implementation of well-controlled procedures, under high-quality standards, for the purification, identification, and characterization of proteins, peptides and various organic molecules.Instruction & guidance of research associates, technical assistants and students. Building and leading teams of research associates and technical assistants to perform protein analytics to support project progression and protocols optimization.Managing all analytical characterization activities in the proteomics unit being accountable for planning, prioritization, and execution of molecule characterization needs. Show less

• Jan 2016 - Dec 2022

Role: Operating the mass spectrometry, HPLC, UPLC and nLC equipment, warranty the correct operation, performing the calibration and maintaining of the different equipment.Sample processing for mass spectrometry, HPLC and UPLC analysis, including proteins and peptides purification, isolation, digestion and preconcentration with procedures based methods of Size Exclusion Chromatography, Reverse Phase, Hydrophilic Interaction Chromatography and Ion Exchange Chromatography. Operation of HPLC, UHPLC and nLC equipment: UltiMate 3000 UHPLC Dionex & UltiMate 3000 nano-UHPLC Dionex (Thermo Fisher Scientific), EASY-nLC 1000 Proxeon (Thermo Fisher Scientific), EASY-nLC Proxeon (Bruker Daltonics), nLC-Proteineer (Bruker Daltonics), Waters 1525 HPLC, Waters 600 HPLC and ÄKTApurifier 10 FPLC (General Electric).Operation of mass spectrometry equipment: Autoflex III smartbeam MALDI TOF/TOF (Bruker Daltonics), nLC ESI-micrOTOF-Q II (Bruker Daltonics), nLC ESI-LTQ-Orbitrap-EDT Velos-Pro (Thermo Fisher Scientific).Operation of biomolecular imager equipment: Typhoon FLA 7000 (General Electric)Electrophoretic Techniques for Protein and Peptides Analysis. Digital Imaging with Image Master 2D platinum (GE) and Decyder 2D (GE) softwares DIGE.Analysis of post-translational modification: phosphorylation and glycosylation.De-novo peptide sequencing. Database processing, software and digital techniques for analysis of samples. Bruker Compass Data Analysis (Bruker Daltonics) and Xcalibur™ Software (Thermo Fisher Scientific)Proteomics data analysis with computational tools such as MaxQuant, Perseus, ProteomeDiscoverer and Peaks.Method validation, processing data and result discussion to assist collaborators on interpretation and publication of research findings.Prepare and present all the required control reports. Show less

• Aug 2018 - Aug 2018

COURSES TAUGHTCenter for Advanced Studies (CEA)/ Structural Biology Center (CBE)Basic Molecular Biology Tools - Electrophoresis

• Oct 2017 - Feb 2018

COURSES TAUGHT Center for Advanced Studies (CEA) / Chemistry CenterAnalytical Chemistry - Chromatography (at graduate level)

• Mar 2017 - Aug 2017

Head in charge of the Proteomics Unit in the absence of the Head for reasons of travel related to academic activities.

• Mar 2016 - Aug 2016

Head in charge of the Proteomics Unit in the absence of the Head for reasons of travel related to academic activities.

• Feb 2012 - Jan 2016

Focus in Organic Chemistry, Chemistry of Marine Natural Products and Proteomics.Purification & isolation of organic compounds and peptide/proteins by PTLC/CC & HPLC with procedures based on chromatography methods : Normal-Phase, Reverse-Phase, Hydrophilic-Interaction (HILIC), Hydrophobic-Interaction (HIC), Ion-Exchange (IEX) and Size-Exclusion/Gel-Permeation (SEC/ GPC). Electrophoretic TechniquesCharacterization Techniques : FTIR, MS, UV/Visible, NMR (1D/2D), LC-MS/MS (DDA/SRM) and CG-MS. Bioassays: MTT, IC50, antibiogram, cytotoxicity Show less

Teacher Assistant

• Mar 2011 - Jul 2011

Chemistry Teacher Courses Taught: Chemistry I and Chemistry II Chemistry Teacher Courses Taught: Chemistry I and Chemistry II

Academic trainer

• Sep 2007 - Nov 2010

Academic trainer of the Laboratories: General Chemistry, Physical Chemistry, Instrumental Analysis, Inorganic Chemistry. Development of collaboration activities with the Chairs and / or Departments in teaching and research, as well as in specific administrative tasks established by those under the supervision of the professors. Attend and advise the groups of students assigned to them, in accordance with the explicitly given instructions and taking into account the particular modalities of the subjects in question. Bibliographic review and preparation of teaching material Show less