Scientist I

• Jan 2022 - Present

Associate Biologist

• May 2020 - Jan 2022

• Perform biological assays to determine efficacy of small molecules• Gain cell culture experience through working with mammalian cell lines to plate for cytotoxicity assays, biological assays and harvesting/lysing cells for western blots• Conduct western blots and gel electrophoresis• Maintain laboratory environment by weekly cleaning of equipments and complete weekly inventory of lab consumables • Work and communicate effectively in a team to cohesively provide accurate results

Laboratory Technician

• Apr 2019 - Nov 2019

• Conducted cell culture experiments to perform CRISPR/Cas9 experiments on mouse embryonic stem cells and differentiate stem cells to spinal motor neurons• Characterized effect of the mutant stem cells generated through qPCR, immunocytochemistry and confocal imaging • Handled laboratory mice by weaning, ear notching and genotyping mice (after DNA extraction) • Trained 3rd & 4th year undergraduate students with lab maintenance, lab bench techniques (such as transformation, DNA & RNA extraction) and cell culture experiments (plating, maintaining, subculturing and freezing embryonic stem cells)

Work Study Student

• Sep 2018 - Apr 2019

• Maintained lab by autoclaving pipette tips, water and removing biohazardous waste • Prepared buffers and mediums for cell culture experiments• Aided graduate students with lab experiments such as PCR, gel electrophoresis, and DNA & RNA extraction