Experience

Manchester Institute of Biotechnology

• United Kingdom
• Research Services
• 1 - 100 Employee

• Postdoctoral Research Associate

  • Feb 2023 - Present

  Engineering of synthetic human chromosomes. Engineering of synthetic human chromosomes.



Newcastle University

• United Kingdom
• Higher Education
• 700 & Above Employee

• Postdoctoral Research Associate

  • Apr 2021 - Jan 2023

  NAD as a substrate for transcription initiation in E.coli. NAD as a substrate for transcription initiation in E.coli.



University of Aberdeen

• United Kingdom
• Higher Education
• 700 & Above Employee

• Researcher PHD Student

  • Jan 2017 - Apr 2021

  Synthetic biology to engineer translation during heterologous protein expression.Efficient translation is centrally important to the expresison of foreign proteins during biotechnological produciton of biologics, such as therapeutic proteins. During heterologous gene expression, the translation system can frequntly become stressed through depletion of key intermediates such as tRNAs and translation factors. To better understand translation systrem 'health', and the maintenance of a balanced gene expression system, mathematical models of translation are being used to understand the supply and demand of key intermediates such as charged tRNAs. I'm interested in understanding how foreign gene sequences impose stress on the host gene expression system, how that stress can be predicted using mathematical modelling, and managed using synthetic biology engineering of the host cell. Show less

• PHD Researcher

  • Jan 2017 - Mar 2021



Indian Institute of Horticultural Research

• Bangalore

• Research Scholar

  • Nov 2014 - Dec 2016

  Worked as Junior Research Fellow, project entitled “Development of high- throughput nanobiosensor for the detection of Salmonella spp. In food”at Indian Institute Of Horticulture Research (IIHR) in department of Post Harvest Technology. Foodborne diseases is one of commonest causes of death in developing countries and Salmonella is one of the commonest cause of foodborne diseases. It has become an major challenge to our health care system and also can have severe impact on society. Conventional microbiological methods and biochemical identification are time-consuming and laborious, while immunological or nucleic acid-based techniques require extensive sample preparation and are not amenable for on-site detection. Biosensors have shown tremendous promise to overcome these limitations . The objective of this project is to develop and validate a high-throughput; simple, sensitive and specific nanoparticle based ready-to-use diagnostic kit for the detection of Salmonella in food. We propose to fabricate a nanodiagnostic device, viz., Aptamer-Nanoparticle Strip Biosensor , to be tested for their suitability for specific end uses based on detection limit. Show less



Chromous Biotech

• Bangalore

• Scientist

  • Jun 2014 - Nov 2014

   Gene Cloning; cDNA library/cloning, Syenthetic gene syentheis, Development of constructs, Suppression Subtractive Hybridization(SSH) libraries, Cloning of upstream/down stream regions, Cloning of expression vectors and expression of recombinant proteins.  Development Of Mutants; Site directed mutagenesis, Random Mutants, Knockout experiements.  Genotyping; Development of microsatellite libraries. Performing marker specific PCR’s, Analysis of polymorphism.  Western Blot, Southern Hybridization, Restriction digestion, Ligation, Invitro transcription, Isolation of genomic DNA/RNA, Plasmid mini prep, Bacterial transformation, Primer designing, DNA sequencing, Protein purification, Chromatography, Construction of DNA ladder.  Plant Molecular Biology; Point of integration, Copy number analysis, Gene expression analysis.  Real Time PCR  dsRNA Show less



Elbit Labs

• Bangalore

• Microbiologist

  • Nov 2013 - May 2014

   Primer designing, Gel elution, Gene Cloning, Bacterial Transformation.  Blood culture, Urine culture, Cell culture and assays.  Isolation of genomic DNA, RNA from bacterial cells and blood, tissue, plasmid isolation. HIV, HBsAg, HCV by ELISA.  Identification of Mycobacterium Tuberculosis Complex DNA, Non Mycobacterium Tuberculosis by real time PCR, Protein Purification.  Determination of autoantibodies in autoimmune diseases by immunofluorescence, Tumour markers, Hormones Hepatitis markers, Cardiac markers.  Western blot, Southern hybridization, HPLC, Quantiferon TB gold test, Anti nuclear antibody profile, ELISA, Real Time PCR, Flow Cytometry, CD counts and HLA B27 assays, Immuno histochemistry,  Maintaining all internal and external quality controls (EQAS BIO RAD U.S.A) and ILQA using ATCC strains. Show less



Santosh Diagnostic

• Bangalore

• Medical Laboratory Technologist

  • Mar 2006 - Jul 2007