Raquel Carvalho

Team Lead at Wild Bioscience
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Contact Information
Location
Colney, England, United Kingdom, UK
Languages
  • English Native or bilingual proficiency
  • Portuguese Native or bilingual proficiency
  • Spanish Elementary proficiency

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Sofia Carvalho

I have worked with Raquel for over eight years, first at Instituto Gulbenkian de Ciencia, and then at the University of Florida. Raquel merges innovation with superior technical quality. She is detail-oriented and persistent, and has interpersonal skills that make her a great coworker.

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Experience

    • United Kingdom
    • Biotechnology Research
    • 1 - 100 Employee
    • Team Lead
      • Sep 2022 - Present
    • United Kingdom
    • Biotechnology Research
    • 1 - 100 Employee
    • Researcher II
      • Aug 2019 - Mar 2022
    • United Kingdom
    • Higher Education
    • 700 & Above Employee
    • Research Associate
      • Jan 2017 - Aug 2019

      Main project: improving rice tolerance to drought and salinity stress, in a collaboration with the International Rice Research Institute (IRRI), in the Philippines. Within the scope of the project, I independently implemented a CRISPR/Cas9 based genome editing platform in rice, for routine use in the lab, and then transferred this knowledge to IRRI. Main project: improving rice tolerance to drought and salinity stress, in a collaboration with the International Rice Research Institute (IRRI), in the Philippines. Within the scope of the project, I independently implemented a CRISPR/Cas9 based genome editing platform in rice, for routine use in the lab, and then transferred this knowledge to IRRI.

    • United States
    • Higher Education
    • 700 & Above Employee
    • Postdoctoral Researcher
      • Jan 2015 - Dec 2016

      Main project: design of a toolbox for the development of strawberry intragenic vectors. Other projects included the identification of novel plant growth regulators, and translation of research results into commercial species, ie. ornamental plant Petunia x hybrida. Established a molecular toolbox for developing cisgenic strawberry and initiated CRISPR/Cas9 genome editing technology for strawberry improvement. Evaluated the response of Arabidopsis transgenic lines to different light… Show more Main project: design of a toolbox for the development of strawberry intragenic vectors. Other projects included the identification of novel plant growth regulators, and translation of research results into commercial species, ie. ornamental plant Petunia x hybrida. Established a molecular toolbox for developing cisgenic strawberry and initiated CRISPR/Cas9 genome editing technology for strawberry improvement. Evaluated the response of Arabidopsis transgenic lines to different light conditions, with the ultimate goal of identifying novel plant growth regulators. Coordinated a paper review on strawberry fruit agroinfiltration for transient assays. Show less Main project: design of a toolbox for the development of strawberry intragenic vectors. Other projects included the identification of novel plant growth regulators, and translation of research results into commercial species, ie. ornamental plant Petunia x hybrida. Established a molecular toolbox for developing cisgenic strawberry and initiated CRISPR/Cas9 genome editing technology for strawberry improvement. Evaluated the response of Arabidopsis transgenic lines to different light… Show more Main project: design of a toolbox for the development of strawberry intragenic vectors. Other projects included the identification of novel plant growth regulators, and translation of research results into commercial species, ie. ornamental plant Petunia x hybrida. Established a molecular toolbox for developing cisgenic strawberry and initiated CRISPR/Cas9 genome editing technology for strawberry improvement. Evaluated the response of Arabidopsis transgenic lines to different light conditions, with the ultimate goal of identifying novel plant growth regulators. Coordinated a paper review on strawberry fruit agroinfiltration for transient assays. Show less

    • Portugal
    • Research Services
    • 100 - 200 Employee
    • Postdoctoral Researcher
      • Jan 2012 - Dec 2014

      As a postdoc, I was able to give continuity to some of the findings that resulted from my PhD research. I performed protein purification and electrophoretic mobility shift assays to validate previously identified candidate direct targets of the SR45 plant-specific splicing factor. I successfully produced and purified recombinant SR45 protein, using it for the gel shift assays. I also generated genetic crosses and transgenic plants for which I subsequently performed phenotypical analyses at the… Show more As a postdoc, I was able to give continuity to some of the findings that resulted from my PhD research. I performed protein purification and electrophoretic mobility shift assays to validate previously identified candidate direct targets of the SR45 plant-specific splicing factor. I successfully produced and purified recombinant SR45 protein, using it for the gel shift assays. I also generated genetic crosses and transgenic plants for which I subsequently performed phenotypical analyses at the level of germination and early seedling development as well as root development, under different stress conditions. Show less As a postdoc, I was able to give continuity to some of the findings that resulted from my PhD research. I performed protein purification and electrophoretic mobility shift assays to validate previously identified candidate direct targets of the SR45 plant-specific splicing factor. I successfully produced and purified recombinant SR45 protein, using it for the gel shift assays. I also generated genetic crosses and transgenic plants for which I subsequently performed phenotypical analyses at the… Show more As a postdoc, I was able to give continuity to some of the findings that resulted from my PhD research. I performed protein purification and electrophoretic mobility shift assays to validate previously identified candidate direct targets of the SR45 plant-specific splicing factor. I successfully produced and purified recombinant SR45 protein, using it for the gel shift assays. I also generated genetic crosses and transgenic plants for which I subsequently performed phenotypical analyses at the level of germination and early seedling development as well as root development, under different stress conditions. Show less

    • PhD student
      • Jan 2007 - Nov 2011

      PhD research project: functional and molecular characterization of SR45, a plant-specific splicing factor in Arabidopsis thaliana. I performed phenotypical analyses of a knockout mutant for the SR45 gene and showed that this splicing factor plays an important role in glucose signaling. Additionally, by performing analyses at both the RNA and protein levels, I determined the involvement of SR45 in the stability of the SnRK1.1 protein kinase, a sugar sensor and central integrator of stress and… Show more PhD research project: functional and molecular characterization of SR45, a plant-specific splicing factor in Arabidopsis thaliana. I performed phenotypical analyses of a knockout mutant for the SR45 gene and showed that this splicing factor plays an important role in glucose signaling. Additionally, by performing analyses at both the RNA and protein levels, I determined the involvement of SR45 in the stability of the SnRK1.1 protein kinase, a sugar sensor and central integrator of stress and energy signaling. Half-way through my PhD I initiated a collaboration with Prof. John Brown from the James Hutton Institute in Dundee, Scotland. This allowed me to travel to Dundee to make use of this group’s well established high-resolution RT-PCR alternative splicing panel to identify endogenous targets of the SR45 splicing factor. Show less PhD research project: functional and molecular characterization of SR45, a plant-specific splicing factor in Arabidopsis thaliana. I performed phenotypical analyses of a knockout mutant for the SR45 gene and showed that this splicing factor plays an important role in glucose signaling. Additionally, by performing analyses at both the RNA and protein levels, I determined the involvement of SR45 in the stability of the SnRK1.1 protein kinase, a sugar sensor and central integrator of stress and… Show more PhD research project: functional and molecular characterization of SR45, a plant-specific splicing factor in Arabidopsis thaliana. I performed phenotypical analyses of a knockout mutant for the SR45 gene and showed that this splicing factor plays an important role in glucose signaling. Additionally, by performing analyses at both the RNA and protein levels, I determined the involvement of SR45 in the stability of the SnRK1.1 protein kinase, a sugar sensor and central integrator of stress and energy signaling. Half-way through my PhD I initiated a collaboration with Prof. John Brown from the James Hutton Institute in Dundee, Scotland. This allowed me to travel to Dundee to make use of this group’s well established high-resolution RT-PCR alternative splicing panel to identify endogenous targets of the SR45 splicing factor. Show less

Education

  • Universidade Nova de Lisboa
    Phd, Plant Molecular Biology
    2007 - 2011
  • University of Lisbon
    Licenciatura, Plant Biology
    2000 - 2005

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