Experience

AlivaMab Biologics

• United States
• Biotechnology Research
• 1 - 100 Employee

• Senior Scientist

  • Jul 2023 - Present

  San Diego, California, United States • In my current role, I am responsible for managing and guiding the work of two research associates, ensuring their productivity and growth within the organization. • One of my significant achievements has been spearheading the implementation of the Electronic Laboratory Notebook (ELN) and Laboratory Information Management System (LIMS) companywide. As a key personnel in this initiative, I have helped streamline data management and improve overall efficiency in our… Show more • In my current role, I am responsible for managing and guiding the work of two research associates, ensuring their productivity and growth within the organization. • One of my significant achievements has been spearheading the implementation of the Electronic Laboratory Notebook (ELN) and Laboratory Information Management System (LIMS) companywide. As a key personnel in this initiative, I have helped streamline data management and improve overall efficiency in our processes. • Furthermore, I have taken on the role of project lead for multiple bispecific engineering and phage display projects. This involves overseeing the planning, execution, and successful completion of these projects, contributing to scientific advancements and breakthroughs. • I actively contribute to the growth of the company by assisting in writing statements of work and designing strategies for new projects. My insights and expertise ensure that these projects have realistic timelines and lead to successful outcomes. • As a valued member of the 5S team, I actively participate in initiatives to enhance efficiency and reduce costs and waste within the organization. My dedication to continuous improvement fosters a culture of innovation and optimization. • Additionally, I play a pivotal cross-functional role, contributing to various aspects of the company's operations, demonstrating my versatility and ability to adapt to different challenges. In summary, my contributions as a manager, cross-functional team member, project lead, and strategic planner have played a vital role in driving the success and growth of the company and helped me grow in my career path.

• Scientist II

  • Jul 2022 - Jun 2023

  San Diego, California, United States • In my role as Scientist II, I held leadership responsibilities for managing a team of two research associates. • Incharge of the molecular biology division, which focuses on various aspects of antibody engineering, reformatting, and humanization. This includes expertise in designing antibodies with bispecific/trispecific features, scFv, FIT-IgG, and VHH domain antibodies, as well as performing cloning and retrieving heavy/light chain sequences from hybridomas. • Successfully optimized… Show more • In my role as Scientist II, I held leadership responsibilities for managing a team of two research associates. • Incharge of the molecular biology division, which focuses on various aspects of antibody engineering, reformatting, and humanization. This includes expertise in designing antibodies with bispecific/trispecific features, scFv, FIT-IgG, and VHH domain antibodies, as well as performing cloning and retrieving heavy/light chain sequences from hybridomas. • Successfully optimized the antibody sequence recovery workflow by implementing high-throughput automated processes, resulting in increased output and shorter timelines. • As the lead scientist, I take charge of designing and cloning antigens for immunization and confirming expression through transient transfections and lentiviral transductions, which facilitate stable pool generation. • I specialize in antibody reformatting to enhance activity and eliminate sequence liabilities, followed by thorough endotoxin testing and expression in mammalian/CHO suspension cultures. • My skillset also includes working knowledge of basic developability assays.

• Scientist I

  • Mar 2021 - Jun 2022

  San Diego, California, United States • Excelled in leading and overseeing crucial aspects of antibody generation and engineering projects. • Responsible for designing, cloning, and synthesizing plasmids for immunization in over 60 projects, which include five bispecific and one trispecific antibody projects. My commitment to meeting project deadlines has ensured the successful progression of these initiatives. • Played a pivotal role in the reformatting of antibody sequences for recombinant expression in more than 70… Show more • Excelled in leading and overseeing crucial aspects of antibody generation and engineering projects. • Responsible for designing, cloning, and synthesizing plasmids for immunization in over 60 projects, which include five bispecific and one trispecific antibody projects. My commitment to meeting project deadlines has ensured the successful progression of these initiatives. • Played a pivotal role in the reformatting of antibody sequences for recombinant expression in more than 70 projects, contributing to the development of advanced antibody formats. • Continuously seeking improvement, I have optimized and modified protocols for hybridoma sequencing and cloning workflows, resulting in cost reduction and enhanced efficiency. • Successfully retrieved antibody sequences from hybridomas using RACE/Sanger sequencing in over 35 projects. • Managed and mentored research associates and undergraduate students, fostering their growth and contributing to the development of the next generation of scientific talent. My dedication to excellence and leadership skills have been essential in achieving success in these endeavors and ensuring the advancement of biomedical research.



UC San Diego

• United States
• Higher Education
• 700 & Above Employee

• Assistant Project Scientist

  • Mar 2018 - Mar 2021

  La Jolla, CA • Collaborated with a colleague (and published in Cell Reports) to understand the mechanism showing role of enhancers in gene dysregulation during cisplatin resistance in ovarian cancer cells. • Contributed in deciphering roles of an African ancestry variants in activating prostate cancer associated long non-coding RNA hub (published in Nature communications and Journal of the National Cancer Institute). • Performed ATAC-seq experiments and GRO-seq sample preparation for a study showing… Show more • Collaborated with a colleague (and published in Cell Reports) to understand the mechanism showing role of enhancers in gene dysregulation during cisplatin resistance in ovarian cancer cells. • Contributed in deciphering roles of an African ancestry variants in activating prostate cancer associated long non-coding RNA hub (published in Nature communications and Journal of the National Cancer Institute). • Performed ATAC-seq experiments and GRO-seq sample preparation for a study showing estrogen-dependent phase separation of enhancers in breast cancer cells (published in Nature Structural and Molecular Biology). • Modified protocol for ATAC-seq library preparation to prepare three times more sample with same reagents. • Established the differential role of estrogen receptor alpha in the regulation of acute and chronic regulation of enhancers by generating stable line in breast cancer cell line viz. MCF7. • Generated cells line with deletion and point mutations (LnCap cells) using the CRISPR-Cas9 system and screened using ddPCR; to study the role of SNPs via loss of transcription factor binding site; in the regulation of long non-coding RNAs.

• Post-Doctoral Fellow

  • Feb 2013 - Feb 2018

  La Jolla, CA, USA • Confirmed the histone modification landscape and transcription factor occupancy in the SNP region using ChIP-seq assays. • Deciphered the chromatin architecture surrounding the SNP using the 4C assays in wild-type and knockdown cells. • Developed a method to isolate protein complexes associated with nuclear RNAs. • Collaborated with a colleague to characterize innate immune response pathway to dsRNA, implicated in the host response to viral infection and inflammation, using ATAC-seq… Show more • Confirmed the histone modification landscape and transcription factor occupancy in the SNP region using ChIP-seq assays. • Deciphered the chromatin architecture surrounding the SNP using the 4C assays in wild-type and knockdown cells. • Developed a method to isolate protein complexes associated with nuclear RNAs. • Collaborated with a colleague to characterize innate immune response pathway to dsRNA, implicated in the host response to viral infection and inflammation, using ATAC-seq and reporter assays.



Salk Institute for Biological Studies

• United States
• Research Services
• 700 & Above Employee

• Post-Doctoral Fellow

  • Mar 2012 - Jan 2013

  La Jolla Worked on understanding the mechanism of alternative splicing and transcriptional regulation.



Indian Institute of Science Education and Research (IISER), Pune

• India
• Research Services
• 700 & Above Employee

• Research Associate

  • Apr 2010 - Feb 2012

  Pune, India ChIP-seq using Applied Biosystems high-throughput sequencer SOLiD 4.



National Centre For Cell Sciences

• India
• Biotechnology Research
• 1 - 100 Employee

• Senior Reserach Fellow (SRF)

  • Jan 2007 - Mar 2010

  Pune Area, India Established the role of histone 3 lysine 56 acetylation in DNA damage in mammalian cells • Proved conclusively, for the first time that histone 3 lysine 56 is acetylated upon DNA damage in mammalian cells; using small molecule inhibitors (methyl methane sulfonate, camptothecin, hydroxyurea) and gamma irradiation. • Identified the causative acetyltransferase and deacetylase enzymes. • Identified the role of H3K56ac in DNA damage pathways and its genome-wide effects by ChIP-on-chip… Show more Established the role of histone 3 lysine 56 acetylation in DNA damage in mammalian cells • Proved conclusively, for the first time that histone 3 lysine 56 is acetylated upon DNA damage in mammalian cells; using small molecule inhibitors (methyl methane sulfonate, camptothecin, hydroxyurea) and gamma irradiation. • Identified the causative acetyltransferase and deacetylase enzymes. • Identified the role of H3K56ac in DNA damage pathways and its genome-wide effects by ChIP-on-chip assays. Deciphered the role of SATB1 in establishing euchromatic and heterochromatic regions in the nucleus in T cells • Identified genomic regions bound by SATB1 using ChIP and electrophoretic mobility shift assays. • Demonstrated methylation of SATB1 for first time; identified effector enzymes & conclusively deciphered residues methylated. • Prepared highly purified protein domains of SATB1 and generated affinity purified antibodies in rabbit. Collaborative work with colleagues • Performed key experiments in establishing the transcriptional roles of SATB1 phosphorylation and acetylation. • Assisted in decoding role of SATB1/beta-catenin pathway in Th cells differentiation in a wnt-dependent manner. • Contributed in evaluating the role of STAB1 and Alu-like motifs in HIV integration in T cells. Show less



National Center for Cell Science

• Research Services
• 1 - 100 Employee

• Junior Research Fellow (JRF)

  • Dec 2004 - Dec 2006

  Pune Area, India Worked towards Ph.D. degree studying post-transnational medications of SATB1, chromatin organizer and transcription factor.



Himachal Pradesh University , Shimla

• India
• Higher Education
• 1 - 100 Employee

• M.Sc. (Biotechnology)

  • Jul 2002 - Nov 2004

  Shimla Area, India Isolated and characterized production and assay conditions for bacterial strains producing pectinolytic enzymes useful for industrial applications like juice clarification. Characterized the production of lipase by various bacterial strains. Wrote an extensive review of bacterial pectinolytic enzymes. The review is one of the ost cited review on pectinoytic enzymes (1250+ citations)



M. D. S. University, Ajmer, INDIA

• B.Sc.

  • Jul 1998 - Jun 2002

  Sri Ganganagar, Rajasthan, INDIA Received B.Sc. degree in chemistry, botany and zoology.