Rama Shivakumar
Manager, Technical Applications at MaxCyte, Inc.- Claim this Profile
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Bio
Experience
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MaxCyte, Inc.
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United States
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Biotechnology
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1 - 100 Employee
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Manager, Technical Applications
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Nov 2020 - Present
Technical Support • Leads technical discussions with MaxCyte scientists and external parties to facilitate pre-sales technology positioning, demo planning and post sales technical support. • Serves as subject matter expert to support the FAS and Sales teams • Works with FAS leadership to create a demo data base • Serves as a member of the company’s cross-functional core team that reviews the design and system requirements for the new product development. • Participates in the… Show more Technical Support • Leads technical discussions with MaxCyte scientists and external parties to facilitate pre-sales technology positioning, demo planning and post sales technical support. • Serves as subject matter expert to support the FAS and Sales teams • Works with FAS leadership to create a demo data base • Serves as a member of the company’s cross-functional core team that reviews the design and system requirements for the new product development. • Participates in the verification and validation of the consumables and software • Participates in generating data that will promote customers’ broad adoption of MaxCyte’s technology. • Assists the legal team with the evaluation of intellectual property filings and contractual documents that require scientific input Scientific Communication: • Synthesizes complex data drawn from publications, customers, collaborators and the internal R&D team to provide the technical focus for writers developing marketing materials. • Delivers scientific talks to promote the MaxCyte technology at scientific meetings, “lunch-and-learn” gatherings, and global Sales meetings • Aligns recent trends in science with MaxCyte’s life sciences strategy by following current literature • Attends select scientific conferences to network with leading scientists and key opinion leaders in order to identify new areas of applications for MaxCyte’s technology Training and Development: • Serves as a scientific subject matter expert for various applications of the MaxCyte platform such as T cell engineering, NK cell engineering, CAR-T Therapies, Gene editing and protein production • Assists in the scientific on-boarding of new cross functional team members • Directs the Scientific Journal Club- a forum for the FAS and the internal R&D team to expand their scientific knowledge and disseminate ideas through the introduction and discussion of new and pertinent scientific research published in journal articles
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Customer Applications Specialist and Research Scientist
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Jun 2018 - Nov 2020
Strategic Marketing: • Contributes to creating compelling scientific and technical content in the areas of CAR Therapies, T cell engineering, NK cell engineering, Gene Editing and Protein Production for use in application notes, white papers, posters, presentations, case studies, manuals and other forms for materials needed for Sales, R&D and corporate purposes. • Manages and curates the scientific publications database. • Collaborates with the sales, field applications and in in house… Show more Strategic Marketing: • Contributes to creating compelling scientific and technical content in the areas of CAR Therapies, T cell engineering, NK cell engineering, Gene Editing and Protein Production for use in application notes, white papers, posters, presentations, case studies, manuals and other forms for materials needed for Sales, R&D and corporate purposes. • Manages and curates the scientific publications database. • Collaborates with the sales, field applications and in in house R&D teams to gain understanding of the current market trends. • Trains and educates the sales, marketing and field teams so that they can sell effectively with confidence and credibility. • Works hand in hand with marketing and R&D to position the company’s data to protect and promote sales of products. • Researches and prepares the write up and review of publications for key symposia and meetings. • Works hand in hand with marketing to identify and strategize plans for key symposia and meetings that result in lead generation. • Presents overview of company’s capabilities at conferences. Product Development: • Member of the core team that reviews new product system requirements (software) and processing assemblies to be used in conjunction with MaxCyte’s ExPert electroporation device. • Verifies and validates the ExPert platform and the processing assemblies • Working hand in hand with the engineering team, ensures that requirements and processes are delivered in a timely manner. • Working with the engineering team, tests the processing assemblies and assess the release criteria before commercial use.
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Research Scientist
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Jun 2013 - Nov 2020
Customer Facing Role: • Conducts technical demos in the US, UK, Europe and India • Conducts demos for cell therapy, protein production, viral vector production and vaccine development. • Relays tech transfer of information related to the use and expansion of applications of the MaxCyte GT system for the generation of DC vaccines—for example to Life Cell, India. • Relays tech transfer of information related to the use and expansion of applications of the MaxCyte GT system for the… Show more Customer Facing Role: • Conducts technical demos in the US, UK, Europe and India • Conducts demos for cell therapy, protein production, viral vector production and vaccine development. • Relays tech transfer of information related to the use and expansion of applications of the MaxCyte GT system for the generation of DC vaccines—for example to Life Cell, India. • Relays tech transfer of information related to the use and expansion of applications of the MaxCyte GT system for the development of nutraceuticals—for example to EID Parry, India. R&D Activities: • Independently designs and executes experiments and interprets data in the areas of cell and molecular biology. • Conducts research on developing electroporation parameters and subsequent cell culture and assay conditions for primary (NKcells, Tcells, DC), adherent, suspension cells, insect cells and algal cells. • Conducts R&D of a DC vaccine using the MaxCyte GT platform for clinical use. Worked on the electroporation and culture of dendritic cells and subsequent analysis of the product. • Possesses extensive hands on knowledge of R&D of AAV and Lentiviral production using MaxCyte’s STX platform. • Possesses extensive hands on knowledge of R&D of mammalian protein production and process development-tested MaxCyte’s proprietary feed strategies and commercially available media/feed combinations in CHOs cells, HEK cells, ExpiCHO cells, insect cells.
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Research Associate IV
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Jun 2007 - Jun 2013
• Optimization of Lentiviral production • Validation of large scale electroporation in K562 cells • Conducted large scale feasibility experiments to load ZFN mRNA for knocking out CCR5 • Optimization of Electroporation protocols for multiple cell types in Protocol Development program • Optimization of transfection parameters of Algal cells • Assisted in animal experiments testing anti angiogenesis agents • Optimization of electroporation conditions for Sleeping Beauty… Show more • Optimization of Lentiviral production • Validation of large scale electroporation in K562 cells • Conducted large scale feasibility experiments to load ZFN mRNA for knocking out CCR5 • Optimization of Electroporation protocols for multiple cell types in Protocol Development program • Optimization of transfection parameters of Algal cells • Assisted in animal experiments testing anti angiogenesis agents • Optimization of electroporation conditions for Sleeping Beauty Transfection in CD34+ cells
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Research Associate II/III
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May 2001 - Jun 2007
Sponsored Research Agreements: • Assisted the technical lead in the feasibility studies demonstrating the use of MaxCyte GT platform in various clinical/non clinical applications • Cloned a number of genes- IL2, CD40L, GFP, SMAR elements intovector backbones so they can be used as transfection controls • Mammalian Cell culture of primary cells, HEK cells, 10T1/2 fibroblasts • Performed all the molecular Biology techniques like Western Blots, ELISAS, PCR, Cloning
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Education
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Indiana University School of Medicine
Master's degree, Molecular Biology -
Bangalore University
Master's degree, Botany/Plant Biology -
Kerala University, Thiruvananthapuram
Bachelor's degree, Botany/Plant Biology