Experience

Tessera Therapeutics

• United States
• Biotechnology Research
• 200 - 300 Employee

• Scientist

  • Aug 2021 - Present



Sanofi

• France
• Pharmaceutical Manufacturing
• 700 & Above Employee

• Scientist

  • May 2020 - Aug 2021

  • Design constructs in-silico, perform a range of biophysical and biochemical experiments for lead candidate optimization and provide models to better understand mechanism of action • Coordinate efforts to support in-vivo characterization of lead candidates in Hemophilia mouse models • Provide biophysical and biochemical support for various Rare disease programs • Continue to guide junior team members and coordinate lab activities to meet project timelines and progress • Design constructs in-silico, perform a range of biophysical and biochemical experiments for lead candidate optimization and provide models to better understand mechanism of action • Coordinate efforts to support in-vivo characterization of lead candidates in Hemophilia mouse models • Provide biophysical and biochemical support for various Rare disease programs • Continue to guide junior team members and coordinate lab activities to meet project timelines and progress



Bioverativ, a Sanofi company

• United States
• Biotechnology
• 1 - 100 Employee

• Senior Associate Scientist

  • Feb 2017 - May 2020

  Co-lead a bispecific antibody subproject aimed at identifying a human Factor VIIIa mimetic by coordinating efforts across a cross-functional team for the design, expression, and purification of bispecific antibodies and subsequent characterization including various binding studies and enzymatic assays Design and perform binding experiments using label free interaction technologies, including BLI and SPR to biophysically characterize proteins in support of preclinical programs in hemophilia… Show more Co-lead a bispecific antibody subproject aimed at identifying a human Factor VIIIa mimetic by coordinating efforts across a cross-functional team for the design, expression, and purification of bispecific antibodies and subsequent characterization including various binding studies and enzymatic assays Design and perform binding experiments using label free interaction technologies, including BLI and SPR to biophysically characterize proteins in support of preclinical programs in hemophilia, sickle cell, and cold agglutinin disease Develop novel ways to address mechanistic questions on drug candidate behavior in plasma or blood by modifying existing plate-based methods Perform hematologic assays including chromogenic and plasma-based Factor Xa generation, thrombin generation and Rotational thromboelastometry to characterize and select drug candidates for in-vivo screening Design in-vitro biochemical assays to determine kinetic parameters (Vmax, kcat, KM) of trimeric complexes under various reaction conditions Isolate platelet-rich and platelet poor plasma from HemA and normal blood donors and perform plasma-based thrombin generation assays and whole blood ROTEM experiments to evaluate and correlate molecular efficiency under physiological conditions Participate in core team and project team meetings to provide weekly updates, prioritize and plan short-term project goals and share thoughts on strategies Show less Co-lead a bispecific antibody subproject aimed at identifying a human Factor VIIIa mimetic by coordinating efforts across a cross-functional team for the design, expression, and purification of bispecific antibodies and subsequent characterization including various binding studies and enzymatic assays Design and perform binding experiments using label free interaction technologies, including BLI and SPR to biophysically characterize proteins in support of preclinical programs in hemophilia… Show more Co-lead a bispecific antibody subproject aimed at identifying a human Factor VIIIa mimetic by coordinating efforts across a cross-functional team for the design, expression, and purification of bispecific antibodies and subsequent characterization including various binding studies and enzymatic assays Design and perform binding experiments using label free interaction technologies, including BLI and SPR to biophysically characterize proteins in support of preclinical programs in hemophilia, sickle cell, and cold agglutinin disease Develop novel ways to address mechanistic questions on drug candidate behavior in plasma or blood by modifying existing plate-based methods Perform hematologic assays including chromogenic and plasma-based Factor Xa generation, thrombin generation and Rotational thromboelastometry to characterize and select drug candidates for in-vivo screening Design in-vitro biochemical assays to determine kinetic parameters (Vmax, kcat, KM) of trimeric complexes under various reaction conditions Isolate platelet-rich and platelet poor plasma from HemA and normal blood donors and perform plasma-based thrombin generation assays and whole blood ROTEM experiments to evaluate and correlate molecular efficiency under physiological conditions Participate in core team and project team meetings to provide weekly updates, prioritize and plan short-term project goals and share thoughts on strategies Show less



Biogen

• United States
• Biotechnology Research
• 700 & Above Employee

• Associate Scientist II

  • Feb 2015 - Feb 2017

  Develop purification protocols for human coagulation factors and antibodies to advance hematology drug discovery projects Medium-scale transfection and expression of proteins in mammalian cell lines Characterize biochemical properties of proteins using SEC-HPLC, SDS-PAGE, etc Screen antibodies by plate-based assays and generate inhibition curves to rank target antibodies In-vitro labeling of anti-target Abs and early stage screening of cell lines expressing membrane-tethered… Show more Develop purification protocols for human coagulation factors and antibodies to advance hematology drug discovery projects Medium-scale transfection and expression of proteins in mammalian cell lines Characterize biochemical properties of proteins using SEC-HPLC, SDS-PAGE, etc Screen antibodies by plate-based assays and generate inhibition curves to rank target antibodies In-vitro labeling of anti-target Abs and early stage screening of cell lines expressing membrane-tethered proteins using BD FACS Canto II Flow cytometry Show less Develop purification protocols for human coagulation factors and antibodies to advance hematology drug discovery projects Medium-scale transfection and expression of proteins in mammalian cell lines Characterize biochemical properties of proteins using SEC-HPLC, SDS-PAGE, etc Screen antibodies by plate-based assays and generate inhibition curves to rank target antibodies In-vitro labeling of anti-target Abs and early stage screening of cell lines expressing membrane-tethered… Show more Develop purification protocols for human coagulation factors and antibodies to advance hematology drug discovery projects Medium-scale transfection and expression of proteins in mammalian cell lines Characterize biochemical properties of proteins using SEC-HPLC, SDS-PAGE, etc Screen antibodies by plate-based assays and generate inhibition curves to rank target antibodies In-vitro labeling of anti-target Abs and early stage screening of cell lines expressing membrane-tethered proteins using BD FACS Canto II Flow cytometry Show less



Shire

• Japan
• Biotechnology Research
• 700 & Above Employee

• Process Engineer

  • Oct 2013 - Dec 2014

  Executed protein purification process control studies using JMP statistical tool, AKTA Avant and AKTA Explorer Packed small-scale SP Sepharose, MEP and cHA II columns and ran qualification protocols to test packing efficiency Supported QbD risk analysis for Process development including Parameter classification, Parameter Risk Assessment and Process Control Strategy Construction Supported Root-cause investigations, FMEA risk analysis and compiled process control reports… Show more Executed protein purification process control studies using JMP statistical tool, AKTA Avant and AKTA Explorer Packed small-scale SP Sepharose, MEP and cHA II columns and ran qualification protocols to test packing efficiency Supported QbD risk analysis for Process development including Parameter classification, Parameter Risk Assessment and Process Control Strategy Construction Supported Root-cause investigations, FMEA risk analysis and compiled process control reports Performed UF/DF cycling studies and assessed NWP recovery, Flux vs TMP curves and process yields Familiar with cGMP process control solutions such as CAPA, Change control and Investigations Show less Executed protein purification process control studies using JMP statistical tool, AKTA Avant and AKTA Explorer Packed small-scale SP Sepharose, MEP and cHA II columns and ran qualification protocols to test packing efficiency Supported QbD risk analysis for Process development including Parameter classification, Parameter Risk Assessment and Process Control Strategy Construction Supported Root-cause investigations, FMEA risk analysis and compiled process control reports… Show more Executed protein purification process control studies using JMP statistical tool, AKTA Avant and AKTA Explorer Packed small-scale SP Sepharose, MEP and cHA II columns and ran qualification protocols to test packing efficiency Supported QbD risk analysis for Process development including Parameter classification, Parameter Risk Assessment and Process Control Strategy Construction Supported Root-cause investigations, FMEA risk analysis and compiled process control reports Performed UF/DF cycling studies and assessed NWP recovery, Flux vs TMP curves and process yields Familiar with cGMP process control solutions such as CAPA, Change control and Investigations Show less



Molecular Response

• Greater San Diego Area

• Intern

  • Aug 2013 - Oct 2013

  Isolated DNA and extracted whole cell proteins from FFPE tumor samples to be shipped to external organizations Cultured and maintained seven different cell lines (adherent, suspension and hybridoma) for IL-6 cell proliferation and beta-galactosidase assays Designed and performed qPCR assays to identify specific tumor markers for early diagnosis of adenocarcinoma and, pancreatic and colorectal tumors Isolated DNA and extracted whole cell proteins from FFPE tumor samples to be shipped to external organizations Cultured and maintained seven different cell lines (adherent, suspension and hybridoma) for IL-6 cell proliferation and beta-galactosidase assays Designed and performed qPCR assays to identify specific tumor markers for early diagnosis of adenocarcinoma and, pancreatic and colorectal tumors



Wayne State University

• Detroit, Michigan

• Intern

  • Jun 2013 - Aug 2013

  Wayne State University, Immunology Study the mechanism of HIV-1 replication in human CD4+ T cells Performed cell culture, transfections, luciferase assays, beta-galactosidase assays and western blotting to characterize the interaction of specific T-cell proteins with HIV-1 proteins Wayne State University, Immunology Study the mechanism of HIV-1 replication in human CD4+ T cells Performed cell culture, transfections, luciferase assays, beta-galactosidase assays and western blotting to characterize the interaction of specific T-cell proteins with HIV-1 proteins



Stemmler Lab

• 540 E Canfield Detroit, MI-48201

• Graduate student

  • Aug 2011 - May 2013

  I worked on two proteins that play vital roles in the eukaryotic FeS cluster biogenesis pathway. I cloned constructs, expressed and purified proteins and characterized them using biochemical and biophysical techniques. My goal was to characterize these proteins and study the sulfide delivery mechanism in the biogenesis pathway. My accomplishments were: - I designed and developed protocols to express and purify two proteins using affinity, SEC and ion exchange chromatography. I also… Show more I worked on two proteins that play vital roles in the eukaryotic FeS cluster biogenesis pathway. I cloned constructs, expressed and purified proteins and characterized them using biochemical and biophysical techniques. My goal was to characterize these proteins and study the sulfide delivery mechanism in the biogenesis pathway. My accomplishments were: - I designed and developed protocols to express and purify two proteins using affinity, SEC and ion exchange chromatography. I also tested and validated biochemical and biophysical assays for the characterization of Drosophila cysteine desulfurase and its partner. - I established titration conditions for protein-protein interaction studies using Isothermal titration calorimetry - I used Circular dichroism to study protein folding changes in different constructs Areas of expertise I have gained expertise in traditional cloning, PCR, primer design, restriction digestion analysis, transformation, sonication, French pressure cell lysis, protein expression, protein detection using western blotting, protein purification using GE Healthcare AKTA-FPLC (gel filtration, ion exchange and affinity chromatography) and traditional bench columns, protein concentration estimation and protein-protein interaction studies using Circular dichroism and Isothermal titration calorimetry. Show less I worked on two proteins that play vital roles in the eukaryotic FeS cluster biogenesis pathway. I cloned constructs, expressed and purified proteins and characterized them using biochemical and biophysical techniques. My goal was to characterize these proteins and study the sulfide delivery mechanism in the biogenesis pathway. My accomplishments were: - I designed and developed protocols to express and purify two proteins using affinity, SEC and ion exchange chromatography. I also… Show more I worked on two proteins that play vital roles in the eukaryotic FeS cluster biogenesis pathway. I cloned constructs, expressed and purified proteins and characterized them using biochemical and biophysical techniques. My goal was to characterize these proteins and study the sulfide delivery mechanism in the biogenesis pathway. My accomplishments were: - I designed and developed protocols to express and purify two proteins using affinity, SEC and ion exchange chromatography. I also tested and validated biochemical and biophysical assays for the characterization of Drosophila cysteine desulfurase and its partner. - I established titration conditions for protein-protein interaction studies using Isothermal titration calorimetry - I used Circular dichroism to study protein folding changes in different constructs Areas of expertise I have gained expertise in traditional cloning, PCR, primer design, restriction digestion analysis, transformation, sonication, French pressure cell lysis, protein expression, protein detection using western blotting, protein purification using GE Healthcare AKTA-FPLC (gel filtration, ion exchange and affinity chromatography) and traditional bench columns, protein concentration estimation and protein-protein interaction studies using Circular dichroism and Isothermal titration calorimetry. Show less



Shiffman Medical Library, Wayne State University

• Metro Detroit

• Student Assistant

  • Jan 2012 - Apr 2013

  Technical assistance for library patrons using computer terminals. Assisted students with queries and allocated books to the Stack section, Journal section and Reference section of the library. Technical assistance for library patrons using computer terminals. Assisted students with queries and allocated books to the Stack section, Journal section and Reference section of the library.



WSU Housing

• Wayne State University

• Student Assistant

  • Sep 2011 - Jan 2012

  Desk Assistant for University Towers front desk. Desk Assistant for University Towers front desk.



GATE-2011

• Student

  • Aug 2010 - Feb 2011

  Was in the 99.6th percentile in GATE-2011 (Life Sciences). All India Rank - 49. Was in the 99.6th percentile in GATE-2011 (Life Sciences). All India Rank - 49.



Bhabha Atomic Research Centre

• Research
• 700 & Above Employee

• Intern

  • Dec 2009 - May 2010

  Studied the biocidal effect of chlorine dioxide on P.aeruginosa and S.aureus biofilms Learned to innoculate and grow biofilms under static and dynamic conditions Optimized a method to generate laboratory-scale chlorine dioxide. Performed Epifluorescent microscopy to visualize morphology and viability of biofilm starter cultures Studied the biocidal effect of chlorine dioxide on P.aeruginosa and S.aureus biofilms Learned to innoculate and grow biofilms under static and dynamic conditions Optimized a method to generate laboratory-scale chlorine dioxide. Performed Epifluorescent microscopy to visualize morphology and viability of biofilm starter cultures