Rafaela Rodrigues
PHD Student at Faculdade de Medicina da Universidade do Porto- Claim this Profile
Click to upgrade to our gold package
for the full feature experience.
Topline Score
Bio
Experience
-
Faculdade de Medicina da Universidade do Porto
-
Portugal
-
Higher Education
-
100 - 200 Employee
-
PHD Student
-
Sep 2022 - Present
-
-
Research Scientist Trainee
-
Dec 2021 - Present
OncoPharma group (CINTESIS/FMUP) Oncology Scientist - Published 2 papers as first-author in peer-reviewed journals;
-
-
-
Unilabs Portugal
-
Portugal
-
Hospitals and Health Care
-
500 - 600 Employee
-
Genetics and Biomolecular Medical Specialist Trainee
-
Mar 2022 - Present
- Developed a project in the epigenetics field: “METILOMA”. - Improved bioinformatic skills using Alamut Visual Plus-SOPHiA GENETICS, Ensembl, OMIM, IGV, NCBI. - Developed a project in the epigenetics field: “METILOMA”. - Improved bioinformatic skills using Alamut Visual Plus-SOPHiA GENETICS, Ensembl, OMIM, IGV, NCBI.
-
-
-
European Association for Cancer Research (EACR)
-
United Kingdom
-
Non-profit Organizations
-
1 - 100 Employee
-
EACR Member
-
Sep 2021 - Present
-
-
-
i3S - Instituto de Investigação e Inovação em Saúde
-
Portugal
-
Research Services
-
400 - 500 Employee
-
MSc Student
-
Jul 2020 - Dec 2021
Master thesis project: Perirenal Adipose Tissue and Clear Cell Renal Cell Carcinoma: "The triad: macrophage-cancer cell-adipocyte" at TMI group. Internship: “Study of Hyperglycemia Influence on Fibrosis Development using animal models: diabetes-cancer link insights” at TMI group. - Developed my Master thesis: "Perirenal adipose tissue and clear cell renal cell carcinoma: the triad cancer cell-macrophage-adipocyte". - The results obtained in this project were used for the development of: (i) my Master Thesis (ii) a PhD Thesis of a FMUP student. - Co-author in 1 book published by Elsevier Inc.; 5 abstracts selected for oral (1) and poster (4) communications at national conferences; Best e-Poster Communication (1) in the Health Sciences area. - Communicated through regular internal presentations in team/division meetings. - Flow cytometry: surface staining of molecules expressed in macrophages, 786-O ccRCC cancer cells, and adipocytes. Using FACS Diva software and posterior analysis performed with FlowJo software. - Monocytes isolation from healthy human buffy coats and macrophages differentiation. - Adipocytes isolation from renal cancer patient samples; adipocytes isolation protocol establishment and optimization. - Cell and tissues culture: 786-O ccRCC cancer cell line, co-cultures with macrophages and tri-cultures used additionally, adipocytes isolated from tissues. - ELISA and cytokine analyses of mono, co and tri-cultures; Hematoxylin and Eosin (H&E), Masson’s Trichrome (MT), and immunohistochemistry (IHC) of perirenal adipose tissue samples from renal cancer patients; DNA extraction; RNA extraction of macrophages, tumour cells, and adipocytes & RT-qPCR; Western-Blotting; Gelatin Zymography; Protein Quantification; Agarose gel electrophoresis. - Quantitative analysis using Quantity One and Image J. -ELISA and flow cytometry results statistically analyzed using SPSS and GraphPad Prism 9 software. Show less
-
-
Education
-
ICBAS- Instituto de Ciências Biomédicas Abel Salazar
Master's degree, Oncology -
Universidade de Trás-os-Montes e Alto Douro
Bachelor of Science - BS, Genética e Biotecnologia -
Faculdade de Medicina da Universidade do Porto
Doctor of Philosophy - PhD, Clinical Laboratory Science/Medical Technology/Technologist