Scientist

• Dec 2021 - Present

Research Staff

• Mar 2019 - Nov 2021

Work responsibilities include: • Independent research project I: My research is focused on understanding the effects of structure-based mutation on TCR-CD3 assembly, cell surface expression, signaling and T cell development and function. I am currently working on the cell based functional assay using Nucleofection transfection method (Amaxa) in Jurkat cells and followed by Flow cytometry. • Besides my research project, I provide assistance to my fellow lab members in – i. Generating Retrovirus soup using 293T cells and checking the efficiency of soups via transduction in 3T3 cells followed by flow cytometry. ii. Providing technical assistance in Real time PCR and associated data analysis, cell-based assays, Flow cytometry (Fortessa, LSR instruments), cell culture maintenance, molecular cloning. iii. Managing day-to-day operation of the laboratory.

Reserach Temp employee

• May 2018 - Nov 2018

Work responsibilities include: • Assisted PI in different scientific projects using technical and scientific skills and designed the experiment, executes and analyzed the results to get the data. • Project: - Study the role of chemokine Signaling in the regulation of fibroblast production though modulation of TGF-beta signaling. • Managed lab routine task including instrument maintenance, managed cell culture (handling primary fibroblast cell lines), monitored liquid Nitrogen. • Provided technical training to undergraduate students. • Participated in lab meeting and presented project progress.

Research Associate II

• Jun 2016 - Feb 2018

Work responsibilities include: • Assisted post-doctoral fellows by performing technical experiments related to their scientific projects. • Independent project: Study the role of microRNAs in epithelial prostate cancer initiation and progression. • Managed routine tasks of research lab including but not limited to instrument maintenance, keep lab data accurately, take care of lab inventory, order new materials. • Participated in lab meetings, seminars and also presented the data. Work responsibilities include: • Assisted post-doctoral fellows by performing technical experiments related to their scientific projects. • Independent project: Study the role of microRNAs in epithelial prostate cancer initiation and progression. • Managed routine tasks of research lab including but not limited to instrument maintenance, keep lab data accurately, take care of lab inventory, order new materials. • Participated in lab meetings, seminars and also presented the data.

Senior Reserch Fellow

• Jul 2008 - Dec 2013

• Investigated differential miRNA expression profiling studies in healthy and diabetic mice model • Studied predicted miRNA and target interaction using different bioinformatics tools and their pathway analysis • Performed in vitro and in vivo experiments to investigate the effect of validated miRNA-target interaction in insulin signaling pathway related to type 2 diabetes • Identified novel miRNAs in human skeletal muscle that play active role in insulin signaling pathway • Performed a wide range of molecular biology experiments, acquired, analyzed and interpreted scientific information objectively

Project Trainee

• Jul 2007 - May 2008

• Investigated physiological and molecular characterization of an auxin inducible, glucose repressible gene encoding expressed protein from Arabidopsis Thaliana • Studied the interaction between sugar and auxin signaling in model plant Arabidopsis Thaliana • Investigated physiological and molecular characterization of an auxin inducible, glucose repressible gene encoding expressed protein from Arabidopsis Thaliana • Studied the interaction between sugar and auxin signaling in model plant Arabidopsis Thaliana