Group Leader

• Oct 2021 - Present

Group Leader of new junior Metabolomics Research group at ISAS, Dortmund. Group Leader of new junior Metabolomics Research group at ISAS, Dortmund.

Sr. Scientist

• Dec 2018 - Oct 2021

Leading operations of EMBL Metabolomics core facility for LC-MS/MS-based metabolomics and lipidomic analysis.

Scientific Officer

• Oct 2016 - Dec 2018

http://www.embl.de/services/core_facilities/metabolomics/members/index.php?s_personId=CP-60021288

Mass spectrometry Scientist

• Apr 2015 - Oct 2016

Post Doctorate Fellow

• Oct 2014 - Mar 2015

Working in IITB Proteomics lab (http://www.bio.iitb.ac.in/~sanjeeva/?page_id=21) for development of HR-LC-MS/MS platforms for Metabolomics and Lipidomics analysis. Working in IITB Proteomics lab (http://www.bio.iitb.ac.in/~sanjeeva/?page_id=21) for development of HR-LC-MS/MS platforms for Metabolomics and Lipidomics analysis.

Research Scientist (DMPK)

• Mar 2013 - Oct 2014

Working in Drug Metabolism and Pharmacokinetics (DMPK) research. Expertise in LC-MS based Metabolite Identification, Cancer Metabolomics, In vitro assay development, Pharmacokinetic Bioanalysis and Drug Metabolism studies to support Drug Discovery and Development Working in Drug Metabolism and Pharmacokinetics (DMPK) research. Expertise in LC-MS based Metabolite Identification, Cancer Metabolomics, In vitro assay development, Pharmacokinetic Bioanalysis and Drug Metabolism studies to support Drug Discovery and Development

CSIR-Nehru Post-doctoral Fellow

• Nov 2010 - Mar 2013

High Resolution Mass spectrometry based 'Metabolomics guided Proteomics' to understand biosynthesis and elucidated biotranformation pathways. Biotransformation studies on various substrates using novel enzymatic systems from rodent microsomes, microbial extracts, recombinant enzymes and transgenic plants. Further expression and purification of novel genes and enzymes involved in biosynthesis of industrially important terpenoids. Metabolomic studies on effect of drug response on Cancer Cell Metabolism using Mass Spectrometry to understand mechanism actions. Show less

Researcher

• Aug 2007 - Aug 2010

Metabolomics and pharmacometabolomics profiling of human urine and plasma samples.To build statistical models using metabolomics data for prediction of drug response.Discover novel metabolite biomarkers of drug response and disease progression.LC-MS based quantification of drug and their metabolites for pharmacokinetic analysis from blood and urine samples.

PhD student/Doctorate Fellow

• Aug 2007 - Aug 2010

PhD in Pharamcometabolomics with expertise in LC-MS, Mass spectroscopy. Pharmacokinetics, Clinical Pharmacology.• Lab set-up and monitor activities for generation of metabolic profile data from biofluids, plasma, urine samples using liquid chromatography-mass spectrometry (LC-MS)• Preparation of method development and validation SOP, protocol for Targeted and Global Metabolite Profiling analysis.• LC-MS, GC-MS and UPLC-MS/MS analysis human urine and plasma samples for Metabolomic profiling (endogenous metabolites).• Statistical analysis like PCA, PLS, PLS-DA and OPLS modeling of Metabolomics data for predicting disease progression, drug response and search metabolite biomarkers.• Pharmacokinetic analysis of drug concentration in human plasma and whole blood.• Assemble data and writing up results for SCI publications in high impact journals. Supervising and training to master and project students on metabolomic analysis Show less

Officer

• 2006 - 2007

LC-MS, GC, GC-MS and HPLC method development for generic drugs, impurity profiling, identification of unknown impurities for DMF filings. Validation of analytical methods and Technology transfer to plant QC. Co-ordinate and Support Medicinal chemistry team. Physicochemical Profiling of NCEs. LC-MS, GC, GC-MS and HPLC method development for generic drugs, impurity profiling, identification of unknown impurities for DMF filings. Validation of analytical methods and Technology transfer to plant QC. Co-ordinate and Support Medicinal chemistry team. Physicochemical Profiling of NCEs.

Analytical Research Officer

• Jan 2004 - May 2006

API and intermediate anayisis by HPLC, GC and wet chemical analysis to generate anlaytical specifications and Monographs. Method development and validation to support DMF filings and US-FDA approvals/inspections. API and intermediate anayisis by HPLC, GC and wet chemical analysis to generate anlaytical specifications and Monographs. Method development and validation to support DMF filings and US-FDA approvals/inspections.