Experience

Poseida Therapeutics, Inc.

• United States
• Biotechnology Research
• 200 - 300 Employee

• Senior Research Associate

  • Apr 2022 - Present

  • Develop analytical methods to characterize and quantify nucleic acid using chromatographic techniques (ion-exchange, ion-pair, size exclusion). • Quantify lipid content from nanoparticle-based formulations using HPLC-ELSD. • Develop analytical methods to characterize and quantify nucleic acid using chromatographic techniques (ion-exchange, ion-pair, size exclusion). • Quantify lipid content from nanoparticle-based formulations using HPLC-ELSD.



Baylor College of Medicine

• United States
• Higher Education
• 700 & Above Employee

• Research Assistant II

  • Feb 2016 - Feb 2022

  • Responsible for operation of entire analytical instruments platform at the center for drug discovery. Performed maintenance and troubleshoot of high throughput analytical instruments (UHPLC/Orbitrap/Ion-trap). Trained coworkers in the use of mass spectrometry systems. • Expertise in IP-RP chromatography of nucleic acids. Developed robust separation methods for HPLC purification of nucleic acids in the build of DNA-encoded libraries (DEL). • Ensured the fidelity of DNA sequences (barcodes) by LC/MS before the build of DEL libraries. • Familiar with in vivo pk studies in mice. Conducted dosing and blood collection. • Performed sample preparation in complex biological matrices in support of high throughput DMPK. • Developed analytical methods for small molecule hit validation by affinity selection mass spectrometry. • Performed enantioselective chromatography of racemic compounds using chiralpak IA-IF columns (normal phase HPLC) and developed a viable scale-up system for prep purification. • Collaborated with biologists and medicinal chemists for the purification and analytical characterization of small molecules, oligonucleotides and peptides. • Developed tune files and validated instrument methods for small molecules and biomolecules. • Familiar with multiple analytical instrument’s software (Xcalibur, Tracefinder, OpenLab, Masslynx, Empower) • Managed multi-instrument warranty contracts and coordinated with service engineers across vendors (Thermo, Agilent, and Waters) for efficient operation of the center for drug discovery. Show less



The University of Texas Health Science Center at Houston (UTHealth Houston)

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Senior Research Assistant

  • Aug 2014 - Jan 2016

  • Carried out an in-vitro study of chemotherapy drug paclitaxel in human whole blood using TSQ Vantage MS/MS in the selected reaction monitoring (SRM) mode and cross-validated the paper spray assay against traditional analytical methodology. • Developed a paper spray mass spectrometry assay for evaluation of efficacy of generic liposomal doxorubicin and Doxil® in a human ovarian cancer orthotropic mouse model. • Played a critical role in the design and optimization of disposable sample cartridge for paper spray MS enabling efficient extraction and ionization of analytes from dried blood spots. Show less



MD Anderson Cancer Center

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Research Assistant II

  • Sep 2013 - Jul 2014

  • Developed an atomic absorption spectrometric method for chemotherapy drug cisplatin using Varian AA 240Z Zeeman, GTA 120 atomizer and utilized an ex-vivo human placental perfusion model to predict potential fetal exposure to cisplatin during pregnancy. • Developed and validated assay for the opioids analgesic drug remifentanil using Waters 2695 HPLC with W2489 UV detector and explored the pk parameters of remifentanil in the typical fetal intervention procedures. • Developed and validated assay for the nonsteroidal anti-inflammatory drug indomethacin using Waters ZQ2000 MS detector to determine indomethacin passage to fetus in pregnant mouse model. Show less



University of Houston-Clear Lake

• United States
• Higher Education
• 700 & Above Employee

• Research Assistant

  • Aug 2011 - May 2013

  • Developed analytical methods for the targeted analysis of pharmaceutical and personal care products (PPCP’s) in Houston’s regional wastewater treatment plant using Agilent LC1260 coupled with MSTOF 6224. • Provided hands-on training in wet chemistry skills for undergraduates in biochemistry and environmental analysis laboratory. Responsible for the operation, maintenance and troubleshoot of Agilent LC 1200, MS 6120. • Developed analytical methods for the targeted analysis of pharmaceutical and personal care products (PPCP’s) in Houston’s regional wastewater treatment plant using Agilent LC1260 coupled with MSTOF 6224. • Provided hands-on training in wet chemistry skills for undergraduates in biochemistry and environmental analysis laboratory. Responsible for the operation, maintenance and troubleshoot of Agilent LC 1200, MS 6120.



Biozone Research Technologies Pvt. Ltd

• India
• Biotechnology Research
• 1 - 100 Employee

• Research Intern

  • Jan 2011 - May 2011

  • Isolated biosurfactant producing microorganisms from sewage and oil contaminated soil samples. • Isolated biosurfactant producing microorganisms from sewage and oil contaminated soil samples.



Bioworld Research Technologies

• Research Intern

  • May 2010 - Jun 2010

  • Researched the biodegradation capabilities of Pseudomonas fluorescens and Escherichia coli. • Applicability in real time bioremediation of oil spills studied based on lipase activity. • Researched the biodegradation capabilities of Pseudomonas fluorescens and Escherichia coli. • Applicability in real time bioremediation of oil spills studied based on lipase activity.



Usha Biotech Solutions

• Industrial Trainee

  • May 2009 - Jun 2009

  • Specialized in molecular biology techniques: western blot, RNA/DNA isolation and quantitation, primer design, PCR, cloning and protein purification • Specialized in molecular biology techniques: western blot, RNA/DNA isolation and quantitation, primer design, PCR, cloning and protein purification