Priscilla K.
Manager, Assay Development at MESO SCALE DIAGNOSTICS, LLC.- Claim this Profile
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Bio
Experience
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MESO SCALE DIAGNOSTICS, LLC.
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Biotechnology Research
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500 - 600 Employee
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Manager, Assay Development
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Sep 2020 - Present
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Scientist II
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Jun 2018 - Aug 2020
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Scientist I
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Jun 2016 - Jun 2018
Meso Scale Discovery (MSD) develops, manufactures, and commercializes both assays and instruments for the measurement of molecules in biological samples. MSD's MULTI‑ARRAY technology enables researchers to profile many biomarkers simultaneously in a single sample with high sensitivity, excellent reproducibility, and wide dynamic range. ISO 9001:2015 and ISO 13485:2016 certified. • As project lead, developed and coordinated launch of multiplexed immunoassays for both U-PLEX and V-PLEX… Show more Meso Scale Discovery (MSD) develops, manufactures, and commercializes both assays and instruments for the measurement of molecules in biological samples. MSD's MULTI‑ARRAY technology enables researchers to profile many biomarkers simultaneously in a single sample with high sensitivity, excellent reproducibility, and wide dynamic range. ISO 9001:2015 and ISO 13485:2016 certified. • As project lead, developed and coordinated launch of multiplexed immunoassays for both U-PLEX and V-PLEX product lines, including V-PLEX COVID19 Serology panels • Conducted and planned product feasibility, scale-up, troubleshooting, and post-release monitoring • Coordinated with interdepartmental project team including QC, QA, Marketing and Operations • Prepared and delivered proposals, presentations, marketing collateral and internal reports • Managed and trained research associate- and scientist-level staff
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Intrexon Corporation
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United States
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Biotechnology
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1 - 100 Employee
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Scientist
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Feb 2015 - Dec 2015
Intrexon Corporation creates biologically-based products that improve the quality of life and the health of the planet. The Company's integrated technology suite provides its partners across diverse markets with industrial-scale design and development of complex biological systems delivering unprecedented control, quality, function, and performance of living cells. • Optimized non-viral gene delivery methods for gene control platforms to support CAR-T development • Automated… Show more Intrexon Corporation creates biologically-based products that improve the quality of life and the health of the planet. The Company's integrated technology suite provides its partners across diverse markets with industrial-scale design and development of complex biological systems delivering unprecedented control, quality, function, and performance of living cells. • Optimized non-viral gene delivery methods for gene control platforms to support CAR-T development • Automated high-density multiplexed gene reporter screening in cell lines and primary cells • Improved liquid handling protocols to reduce supply costs and cell cultivation time • Managed day-to-day laboratory operations, inventory, internal presentations, protocols, and internship junior staff Show less Intrexon Corporation creates biologically-based products that improve the quality of life and the health of the planet. The Company's integrated technology suite provides its partners across diverse markets with industrial-scale design and development of complex biological systems delivering unprecedented control, quality, function, and performance of living cells. • Optimized non-viral gene delivery methods for gene control platforms to support CAR-T development • Automated… Show more Intrexon Corporation creates biologically-based products that improve the quality of life and the health of the planet. The Company's integrated technology suite provides its partners across diverse markets with industrial-scale design and development of complex biological systems delivering unprecedented control, quality, function, and performance of living cells. • Optimized non-viral gene delivery methods for gene control platforms to support CAR-T development • Automated high-density multiplexed gene reporter screening in cell lines and primary cells • Improved liquid handling protocols to reduce supply costs and cell cultivation time • Managed day-to-day laboratory operations, inventory, internal presentations, protocols, and internship junior staff Show less
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Virginia Tech
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United States
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Higher Education
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700 & Above Employee
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Postdoctoral Research Associate
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May 2014 - Feb 2015
Department of Biochemistry and Virginia Tech Center for Drug Discovery In collaboration with the Chemistry Department at Virginia Tech, using a bioassay-guided fractionation method, we screen plant derived extracts to isolate novel antimalarial compounds. I am also heavily involved in the identification of molecular targets for promising, highly active compounds. • Developed cell-based assays to evaluate cytotoxicity and mode of action of small molecule inhibitors • Designed… Show more Department of Biochemistry and Virginia Tech Center for Drug Discovery In collaboration with the Chemistry Department at Virginia Tech, using a bioassay-guided fractionation method, we screen plant derived extracts to isolate novel antimalarial compounds. I am also heavily involved in the identification of molecular targets for promising, highly active compounds. • Developed cell-based assays to evaluate cytotoxicity and mode of action of small molecule inhibitors • Designed methods for targeted gene editing using CRISPR/Cas9 and recombination technologies • Independently planned and troubleshot new techniques of metabolite extract preparation from challenging cell samples • Partnered with medicinal chemists in the development of specific inhibitors of parasite metabolic pathways • Trained and supervised junior laboratory members, including full-time research staff
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Research Assistant
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Sep 2013 - Apr 2014
Department of Biochemistry • Conducted screening for antimalarial compounds with a novel mode of action • Employed transgenic cell lines to probe for inhibitors with endocytic activity via fluorescence microscopy and amino acid efflux assays
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Graduate Student Research Assistant
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Aug 2008 - Aug 2013
Department of Biochemistry Investigations into the Nature of the Endosomal System in Plasmodium falciparum • Validated drug targets and their inhibition by small molecules through microscopic image analysis, enzyme, and cell-based assays • Characterized recombinant and native proteins through plate-based fluorogenic enzyme assays and chromatography. • Engineered stable cell lines using non-viral gene editing techniques, including transposon-based… Show more Department of Biochemistry Investigations into the Nature of the Endosomal System in Plasmodium falciparum • Validated drug targets and their inhibition by small molecules through microscopic image analysis, enzyme, and cell-based assays • Characterized recombinant and native proteins through plate-based fluorogenic enzyme assays and chromatography. • Engineered stable cell lines using non-viral gene editing techniques, including transposon-based recombination • Maintained and trained new members on aseptic cell culture technique following BSL-2 requirements • Presented research regularly at professional conferences and university-wide seminars
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Japan Exchange and Teaching Program(me) (JET)
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International Affairs
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700 & Above Employee
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Assistant Language Teacher (ALT)
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Aug 2006 - Jul 2008
Taught courses at: Tsubata Senior High School (石川県立津幡高等学校) Ishikawa Prefectural School for Students with Special Needs (石川県立総合養護学校) • Introduced aspects of English language and American culture to students • Designed and implement engaging lessons and classroom activities Employed by the Ishikawa Prefecture Board of Education. Taught courses at: Tsubata Senior High School (石川県立津幡高等学校) Ishikawa Prefectural School for Students with Special Needs (石川県立総合養護学校) • Introduced aspects of English language and American culture to students • Designed and implement engaging lessons and classroom activities Employed by the Ishikawa Prefecture Board of Education.
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William & Mary
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United States
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Higher Education
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700 & Above Employee
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IT Technician (Level 1)
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Oct 2002 - May 2006
• Solved computer and network issues for faculty, staff and students • Assisted incoming students with the network and phone system during orientation • Solved computer and network issues for faculty, staff and students • Assisted incoming students with the network and phone system during orientation
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Education
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Virginia Polytechnic Institute and State University
Ph.D., Biochemistry -
The College of William and Mary
B.S., Chemistry -
Council Rock (North) High School