Research Intern

• Jun 2023 - Present

Working on the same project for Dr. James Jahng in the lab of Dr. Joseph Wu, this summer I successfully developed two separate fluorescent reporter cell lines using CRISPR HDR. Using a Cas9-guideRNA expression vector and a 3’-overhang dsDNA donor to introduce a fluorescent protein to the C-terminal of an endothelial cell marker, as well as the C-terminal of an EndMT marker Working on the same project for Dr. James Jahng in the lab of Dr. Joseph Wu, this summer I successfully developed two separate fluorescent reporter cell lines using CRISPR HDR. Using a Cas9-guideRNA expression vector and a 3’-overhang dsDNA donor to introduce a fluorescent protein to the C-terminal of an endothelial cell marker, as well as the C-terminal of an EndMT marker

Research Intern

• Jun 2022 - Aug 2022

Worked in the Lab of Dr. Joseph Wu with Dr. James Jahng to study the molecular mechanisms of radiation-induced cardiovascular disease, utilizing iPSC-ECs. Using RT-qPCR, I screened for marker genes for cellular senescence and endothelial-to-mesenchymal transition in iPSC-ECs exposed to X-ray radiation delivered either single or fractionated, in an effort to find up/down regulated genes that could be used for a dual-fluorescent reporter cell line. This cell line could then be used for drug screening to find compounds that diminish the effects of radiation on our vascular system. Show less

Research Intern

• Sep 2020 - Jun 2021

Worked full-time in the lab of Dr. Norbert Huebner with Dr. Michael Muecke to discover function of micropeptides specific to the heart using iPSC-derived cardiomyocytes. Performed experiments using CRISPR, siRNA, and Adeno-associated Viruses to knockout, knockdown, and overexpress genes specific to the heart in iPSC-derived cardiomyocytes that I differentiated myself. Worked full-time in the lab of Dr. Norbert Huebner with Dr. Michael Muecke to discover function of micropeptides specific to the heart using iPSC-derived cardiomyocytes. Performed experiments using CRISPR, siRNA, and Adeno-associated Viruses to knockout, knockdown, and overexpress genes specific to the heart in iPSC-derived cardiomyocytes that I differentiated myself.

Doordash Delivery Driver

• May 2020 - Aug 2020