Research And Development Scientist • Aptamers • Cell-SELEX • Targeted protein degradation

• Jan 2022 - Present

Preclinical Scientist • Drug transporters • Aptamers • Cell-SELEX

• Jan 2021 - Jan 2022

At CIC Bio Gune I executed a project to develop a novel aptamer-guided method for targeted drug delivery in hepatocytes, troubleshooting all technical aspects of the Cell-SELEX methodology, which was designed and employed to discover aptamers capable of specifically recognizing the hepatic bile acid transporter/receptor NTCP. In addition, together with other colleagues, I worked on studying the transcriptional regulation of a transporter protein related with liver cancer and NAFLD/NASH, using DNA pulldown, proteomics and functional assays. Keywords: Project planning and experimental design, BSL2, mammalian cell culture lines, FACS, fluorescence microscopy, cell assay development, gene silencing, gene expression, in vitro assays, proteomics, NGS, aptamers, Cell-SELEX, adenovirus, lentivirus packaging Show less

Preclinical Scientist • Liver metabolism• DILI • cholestasis

• Oct 2016 - Jan 2022

At IIS La Fe I aimed at understanding the molecular mechanisms by which augmentin (amoxicillin-clavulanic acid) and anabolic steroids cause disruption of molecular metabolism of bile acids in the liver and drug-induced liver injury in susceptible individuals, as well as the mechanistic links between gut microbiota and NAFLD (https://goo.gl/rhKHSF). Keywords: primary hepatocytes, drug-induced liver injury, cholestasis, drug transporters, nuclear receptors, adenoviral based protein overexpression, bile acids, anabolic androgenic steroids, toxicogenomics, toxicology. Show less

Bioactive Compounds Database Curator

• Oct 2015 - Feb 2016

• Conducted searches for science-related information stored in a variety of data sources regarding bioactive compounds (including poplyphenols, peptides, oligosaccharides) in the 50 mostly consumed foods in Spain. • Screened 700+ research papers and 20+ books; produced a 300+ entries database. • Identified 5 bioactive compounds with potential for commercialization in food supplements and nutraceuticals. • Conducted searches for science-related information stored in a variety of data sources regarding bioactive compounds (including poplyphenols, peptides, oligosaccharides) in the 50 mostly consumed foods in Spain. • Screened 700+ research papers and 20+ books; produced a 300+ entries database. • Identified 5 bioactive compounds with potential for commercialization in food supplements and nutraceuticals.

Graduate researcher • Adipose tissue biology • Molecular nutrition • Biomarkers

• Oct 2010 - Feb 2016

● Expanded the knowledge on the role of retinoblastoma protein in metabolic regulation ● Collaborated with and performed two research stays at 2 research groups in Spain and Switzerland, leading to 2 joint publications. ● First author of 6 publications in Q1 peer-reviewed journals (Diabetes, American Journal Of Physiology Endoncrindology and Metabolism, Journal of Cellular Physiology, Cellular Physiology and Biochemistry, PlosOne, publications list available at http://goo.gl/cuJTmp ). ● Presented the experimental findings at 4 international meetings. ● Provided hands-on training in molecular biology methods to 4 master students and 1 visiting PhD student. ● Introduced 5 novel techniques in the lab. Keywords: adipose tissue, skeletal muscle, adipocytes, myotubes, metabolism, energy metabolism, fatty acid metabolism, glucose homeostasis, tumor suppressor, animal models, mice, 2D/3D cell culture, cell cultures, primary cell cultures, RNA interference, shRNA, protein interactions, western-blot, immunoprecipitation, immunofluorescence, fluorescence microscopy, cellular assays, functional assays, signaling pathways, FACS, flow cytometry, transfection, transduction, DNA/RNA purification, RT-qPCR, SOP, lentivirus, proximity-ligation assay, kinase assay, vector design, molecular cloning, protein expression, protein purification, protein-protein interactions. Show less

Production Manager, Head of Research and Development

• Feb 2007 - Jun 2009

● Planned, designed and executed projects on new food products, as well as developed SOPs for the ISO 22000 quality system, utilizing sound professional judgment. High degree of interaction with Accounting and Technical support teams. ● Represented the company at 10+ international expositions and established direct contacts with 30+ functional ingredients´ suppliers (whey concentrates and isolates, total milk proteins, hydrocolloids, functional carbohydrates and more). ● Lead a cross-cultural, cross-linguistic and multi-religious team of 50+ people with 5 direct reports, for a company that produced over 40 food products. Worked in a complex, multi-layered matrix organization as well as, within project teams as an independent self-starter with little direct supervision. Show less

Bioinformatician • Epitope prediction • Data integration

• Jan 2000 - Mar 2001

Project lead and coordination for development of an epitope-prediction bioinformatics software prototype, currently used by several companies within the Drug Discovery domain. Project lead and coordination for development of an epitope-prediction bioinformatics software prototype, currently used by several companies within the Drug Discovery domain.