Experience

Kiadis Pharma

• Netherlands
• Biotechnology
• 1 - 100 Employee

• Associate Scientist

  • Oct 2020 - Present



UMCG

• Netherlands
• Hospitals and Health Care
• 700 & Above Employee

• Research Assistant

  • Sep 2017 - Jul 2020

  In vitro and in vivo modeling of novel mutations in cardiocutaneous syndromes. To accomplish this, I generated several induced pluripotent stem cells (iPSC) from patients carrying pathogenic mutations, and ultimately differentiated them into cardiomyocytes and created 3D cardiac tissues. In addition to extensive cell culture, techniques used include microscopy (immunofluorescence/electron microscopy/confocal/fast high-resolution) qPCR, Western blot, SDS-PAGE, Seahorse XF, wide range of biochemical assays, plasmid transfection and viral transduction. Additionally, I phenotyped a knock-out mouse model where we used ECG, MRI, echocardiography, pressure-volume loops and lastly analyzed the organs post-mortem.



UMCG

• Netherlands
• Hospitals and Health Care
• 700 & Above Employee

• Research Intern

  • Nov 2016 - Jun 2017

  We developed a novel in vitro model for arrhythmogenic cardiomyopathy (ACM) using patient-specific cardiomyocytes derived from induced pluripotent stem cells (iPSC), in combination with metabolic maturation and mechanical strain. I created new iPSC from several ACM patients, and worked widely with cell culture, differentiations, protein and RNA analysis. We developed a novel in vitro model for arrhythmogenic cardiomyopathy (ACM) using patient-specific cardiomyocytes derived from induced pluripotent stem cells (iPSC), in combination with metabolic maturation and mechanical strain. I created new iPSC from several ACM patients, and worked widely with cell culture, differentiations, protein and RNA analysis.



UMCG

• Netherlands
• Hospitals and Health Care
• 700 & Above Employee

• Research Intern

  • Feb 2016 - Jun 2016

  We created an in vitro model of endothelial cell and platelet interaction during hibernation-like conditions, where I grew endothelial cells in microfluidic chips. IF-microscopy and FACS were extensively used. We created an in vitro model of endothelial cell and platelet interaction during hibernation-like conditions, where I grew endothelial cells in microfluidic chips. IF-microscopy and FACS were extensively used.



Escuela de Medicina del Tecnológico de Monterrey

• Mexico
• Higher Education
• 1 - 100 Employee

• Research Intern

  • Jun 2014 - Jun 2015

  I standardized the techniques of cell culture, biochemistry assay measurements (e.g.: ELISA) and data analysis for the development of a biopharmaceutical derived from plant extracts to prevent pterygium. I standardized the techniques of cell culture, biochemistry assay measurements (e.g.: ELISA) and data analysis for the development of a biopharmaceutical derived from plant extracts to prevent pterygium.



Nature's Factory

• Mexico
• Food and Beverage Services
• 1 - 100 Employee

• Research And Development Intern

  • Aug 2013 - Jan 2014

  Creation of new food products and reformulation of existing ones. Creation of new food products and reformulation of existing ones.