Pauline Lambert
Biorefinery Scientist Assistant at IBBL (Integrated Biobank of Luxembourg)- Claim this Profile
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Français Native or bilingual proficiency
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Allemand Elementary proficiency
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Anglais Professional working proficiency
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Bio
Experience
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IBBL (Integrated BioBank of Luxembourg)
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Luxembourg
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Research Services
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1 - 100 Employee
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Biorefinery Scientist Assistant
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Jan 2015 - Present
- Fee for services Study managment (Sample processing, Specific Operating Procedure writing, spreadsheet development, Stock and Products for processing management, Operator training) - miRNA/RNA/DNA extraction from diverse biological materials - Reception and tracking of samples in a specific software: Laboratory Informatic Management System (LIMS- Labvantage) - Basic samples processing: blood serum/plasma and urine aliquoting, primary cell culture of fibroblast) - Cell culture (Fibroblasts, Jurkat cells, Establishment of Lymphoblastoïd Cell Line, iPScells production and expansion) and Viability / Subpopulation assessment (BD-FACSverse) - Proficiency testing - Participation in Research studies on Quality control markers - Participation in Norm development (Cell counting method comparison) http://www.ibbl.lu/ Show less
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Luxembourg Institute of Health
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Luxembourg
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Research Services
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300 - 400 Employee
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Internship
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Feb 2014 - Sep 2014
- During theses 8-month internship, I took part in a project developed in the laboratory Retrovirology CRP-Santé in Luxembourg in A.Chevigne and M.Szpakowska Team. - Molecular biology: cloning, use of restriction enzymes, PCR, DNA ligation, agarose electrophoresis gels and purification on gels. - Use of techniques: transfections (lipofectamine), cell culture (U87), clones selection, cell sorting, flow cytometry, fluorescent component binding competition. - Writing a dissertation of 30 pages and oral présentation (20 mins). Show less
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Luxembourg Institute of Health
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Luxembourg
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Research Services
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300 - 400 Employee
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Internship
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Jan 2013 - Feb 2013
- During theses 2-month internship, I took part in a project developed in the laboratory Retrovirology CRP-Health in Luxembourg in A.Chevigne and V. Fievez Team. - Re-discovered and understanding of the world of research, both applied and fundamental. - Observation / understanding of the organization of a research laboratory and missions each. - Use of techniques: cloning, bacterial transformation, production and purification of protein as well as in the field of molecular biology. - Writing a dissertation and oral presentation of internship after the course to a jury of my university. Show less
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Luxembourg Institute of Health
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Luxembourg
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Research Services
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300 - 400 Employee
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Intership
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Apr 2012 - Apr 2012
Observation and manipulation of PCR techniques, transformation, DNA exctraction and sequencing, electroporation and luminescence measuring under the study of the effect of an inhibitor of the NS3 protease / 4a HCV virus on the development cycle and infectivity of the latter. Observation and manipulation of PCR techniques, transformation, DNA exctraction and sequencing, electroporation and luminescence measuring under the study of the effect of an inhibitor of the NS3 protease / 4a HCV virus on the development cycle and infectivity of the latter.
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AUTOLUX SARL
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Luxembourg
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Secrétaire
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Jan 2009 - Jan 2012
One month during each summer season. (July) Make leases, answer the phone, manage a schedule, solve problems in case of emergencies... One month during each summer season. (July) Make leases, answer the phone, manage a schedule, solve problems in case of emergencies...
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Education
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Université de Lorraine
Master BSIS (Biosciences et Ingénierie de la Santé) - Spécialité Génie Cellulaire - mention Bien, Biologie cellulaire et histologie -
Université de Lorraine
Master Cell biology and physiologic bases of therapeutic, Cell/Cellular and Molecular Biology -
Université Henri Poincaré (Nancy I)
Licence sciences du vivant, Cell/Cellular Biology and Histology -
Lycée saint Pierre Chanel
Baccalauréat, Science du vivant et de la santé; biologie; science du vivant; biologie cellulaire; Scientifique