Project Manager I

• Mar 2023 - Present

Research Specialist I

• Feb 2020 - Mar 2023

- Perform and provide zebrafish husbandry and colony management of zebrafish stock and mutant lines - Assist in technical procedures related to aquatic species such as obtaining zebrafish sperm for cryopreservation and in vitro fertilization, isolate tissue samples for genotyping using PCR, facilitating animal breeding, embryo collection, embryo cleaning, staging and fixation, and propagation - Perform various bench-top molecular biology procedures (e. g. PCR, gel electrophoresis, DNA… Show more - Perform and provide zebrafish husbandry and colony management of zebrafish stock and mutant lines - Assist in technical procedures related to aquatic species such as obtaining zebrafish sperm for cryopreservation and in vitro fertilization, isolate tissue samples for genotyping using PCR, facilitating animal breeding, embryo collection, embryo cleaning, staging and fixation, and propagation - Perform various bench-top molecular biology procedures (e. g. PCR, gel electrophoresis, DNA purification, bacterial cultures, restriction digestion, DNA ligation, mRNA isolation, reverse transcription, gel extraction, and in situ hybridization) - Analyze sequence data obtained using various applications to determine correct sequences for plasmids, genotypes, cultures, etc. - Perform DNA, RNA, morpholino micro-injections into 1-cell zebrafish embryos to create transgenic zebrafish lines, knockdown genes, add fluorescent markers, or continue propagation of zebrafish lines - Image various stage zebrafish embryos (1-cell to 5 dpf) using a fluorescent microscope and/or Nikon confocal to look for specific cell types or genotypes - Create, maintain, and adhere to specific Standard Operating Procedures for the lab - Monitor and organize lab inventory, create lab solutions and aliquots, receive supply shipments, and order lab supplies to support daily operations - Monitor lab equipment and lab conditions and perform regular care and maintenance (e. g. place and follow up on maintenance tickets, cleaning, and arranging contracted services) - Assist and train fellow lab members on multiple projects and procedures to support individual researcher’s projects and methods development

Aquatic Specialist III

• Jan 2019 - Feb 2020

- Continue to maintain the responsibilities performed as an Aquatic Specialist II - Conduct all in-house water quality tests, preventative maintenance on Danio rerio and Xenopus spp. life support systems, and daily room checks accurately - Recognize irregular clinical chemistry values for water quality, abnormal animal behavior, and various parasites and immediately report to the facility veterinarian and/or management - Provide day to day instruction to fellow Aquatic Specialist II… Show more - Continue to maintain the responsibilities performed as an Aquatic Specialist II - Conduct all in-house water quality tests, preventative maintenance on Danio rerio and Xenopus spp. life support systems, and daily room checks accurately - Recognize irregular clinical chemistry values for water quality, abnormal animal behavior, and various parasites and immediately report to the facility veterinarian and/or management - Provide day to day instruction to fellow Aquatic Specialist II teammates on tasks that need to be completed as well as provide feedback with questions or concerns presented - Maintain accurate and detailed records regarding water quality parameters, colony health, breeding, and/or housing with an open line of communication between the researchers, facility veterinarian, and management - Adhere to the Standard Operating Procedures outlined and comply with safety policies, procedures, and MSDS guidelines - Clean, reorganize, label, and create master list of items in facility wide storage rooms and closets - Oversee and manage a seven-month facility wide floor replacement project, which includes the tracking of spacing requirements for specific labs, strategic movement of stocked animal housing, open communication with project managers, research staff, and fellow employees on the movement of the aquatic animals, and the cleaning of holding areas before and after the placement of new flooring

Aquatic Specialist II

• Jan 2017 - Jan 2019

- Utilize standard operating procedures for Zebrafish (adult, juvenile, and fry) husbandry, health, and feeding for researchers at the National Institutes of Health - Cultivate and disperse Artemia in an aquatic laboratory to sustain research initiatives - Maintain, harvest, and feed rotifer colonies in order to support fry care - Measure and record dissolved oxygen, pH, and electric conductivity for water quality monitoring - Provide customer service for researchers through… Show more - Utilize standard operating procedures for Zebrafish (adult, juvenile, and fry) husbandry, health, and feeding for researchers at the National Institutes of Health - Cultivate and disperse Artemia in an aquatic laboratory to sustain research initiatives - Maintain, harvest, and feed rotifer colonies in order to support fry care - Measure and record dissolved oxygen, pH, and electric conductivity for water quality monitoring - Provide customer service for researchers through excellent communication

Researcher III

• Jun 2016 - Jan 2017

- Prepped sediment samples from the Florida Keys for laboratory analysis - Extracted and identified foraminifera species from sediment samples - Performed radiocarbon dating and filtered sediment samples from cores in Snipe Key and Biscayne Bay, Florida - Input and analyzed foraminifera data collected from sediment samples using Microsoft Excel, R, and PAST statistical software - Implemented research techniques to analyze and review scientific literature - Worked as a member of… Show more - Prepped sediment samples from the Florida Keys for laboratory analysis - Extracted and identified foraminifera species from sediment samples - Performed radiocarbon dating and filtered sediment samples from cores in Snipe Key and Biscayne Bay, Florida - Input and analyzed foraminifera data collected from sediment samples using Microsoft Excel, R, and PAST statistical software - Implemented research techniques to analyze and review scientific literature - Worked as a member of the project and participated in weekly meetings to share results and assess progress Show less - Prepped sediment samples from the Florida Keys for laboratory analysis - Extracted and identified foraminifera species from sediment samples - Performed radiocarbon dating and filtered sediment samples from cores in Snipe Key and Biscayne Bay, Florida - Input and analyzed foraminifera data collected from sediment samples using Microsoft Excel, R, and PAST statistical software - Implemented research techniques to analyze and review scientific literature - Worked as a member of… Show more - Prepped sediment samples from the Florida Keys for laboratory analysis - Extracted and identified foraminifera species from sediment samples - Performed radiocarbon dating and filtered sediment samples from cores in Snipe Key and Biscayne Bay, Florida - Input and analyzed foraminifera data collected from sediment samples using Microsoft Excel, R, and PAST statistical software - Implemented research techniques to analyze and review scientific literature - Worked as a member of the project and participated in weekly meetings to share results and assess progress Show less

Graduate Teaching Assistant

• Jan 2014 - May 2016

My duties as a graduate teaching assistant include teaching labs associated with core classes, specifically GLY 120 (Environmental Geology) and OCN 150 (Introduction to Oceanography). I am in charge of presenting the information within their lab manuals and teaching them the skills and information necessary in order to complete the labs each week. I also prepare exams, supplementary materials, quizzes, review sessions, and input their grades using the online resource, Blackboard Learn. My duties as a graduate teaching assistant include teaching labs associated with core classes, specifically GLY 120 (Environmental Geology) and OCN 150 (Introduction to Oceanography). I am in charge of presenting the information within their lab manuals and teaching them the skills and information necessary in order to complete the labs each week. I also prepare exams, supplementary materials, quizzes, review sessions, and input their grades using the online resource, Blackboard Learn.

Husbandry Intern

• Sep 2012 - Dec 2012

Cleaning and maintaining exhibits and animal enclosures, maintaining life support systems, observing behavior and habits of animals, preparing diets and feeding animals (sea turtles, jellies, Chondrichthyes, salt water and fresh water fishes, terrapins, etc.), and water quality testing (pH, temperature, copper, nitrate, and salinity) and quarantine procedure experience and knowledge. Cleaning and maintaining exhibits and animal enclosures, maintaining life support systems, observing behavior and habits of animals, preparing diets and feeding animals (sea turtles, jellies, Chondrichthyes, salt water and fresh water fishes, terrapins, etc.), and water quality testing (pH, temperature, copper, nitrate, and salinity) and quarantine procedure experience and knowledge.

Instructor

• May 2012 - Aug 2012

Created lesson plans with a co-instructor for week long camp programs for children aged 7-8. Used knowledge of marine biology and geology to educate and motivate youth to appreciate various aspects of marine life. Communicated with parents on child's progress and answered questions presented. Created lesson plans with a co-instructor for week long camp programs for children aged 7-8. Used knowledge of marine biology and geology to educate and motivate youth to appreciate various aspects of marine life. Communicated with parents on child's progress and answered questions presented.