Experience

Laboratory of Biology and Modeling of the Cell

• France
• Research Services
• 1 - 100 Employee

• PhD Student

  • Oct 2018 - Present

  Région de Lyon, France



L'École Normale Supérieure de Lyon, LBMC

• Research Trainee

  • Jan 2018 - Jul 2018

  Lyon Area, France Study of the chromatine 3D conformation in the regulation of the transcription-alternative splicing coupling transcription-épissage alternatif - qChIP - qRT-PCR - Chromosome Conformation Capture assays (3C, 4C) - CRISPR/dCas9 (CRISPRi, CRISPRa, dCas9-tethering) - co-IP



Pure Biologics

• Poland
• Biotechnology Research
• 1 - 100 Employee

• Research associate intern

  • Jun 2017 - Aug 2017

  Wroclaw, Lower Silesian District, Poland Cloning, expression and purification of recombinant proteins. - Expression vectors construction (PCR, Restriction Free Cloning, Nanodrop, agarose gel electrophoresis, plasmid DNA purification) - Plasmid production in bacterial systems (transformation) - Optimization of protein expression conditions in bacterial systems (E. coli, expression profile analysis) - Protein purification (HPLC, IEX, IMAC) - Protein characterization (SDS-PAGE, Western-Blot) - Lab book and project… Show more Cloning, expression and purification of recombinant proteins. - Expression vectors construction (PCR, Restriction Free Cloning, Nanodrop, agarose gel electrophoresis, plasmid DNA purification) - Plasmid production in bacterial systems (transformation) - Optimization of protein expression conditions in bacterial systems (E. coli, expression profile analysis) - Protein purification (HPLC, IEX, IMAC) - Protein characterization (SDS-PAGE, Western-Blot) - Lab book and project reports writing, LIMS, Image Lab (Bio-Rad) Show less



Institut de Génétique Moléculaire de Montpellier (IGMM)

• Research
• 1 - 100 Employee

• Research on transcription in living cells - E.Bertrand's research team

  • Jun 2016 - Jul 2016

  Région de Montpellier, France PCR screening of CRISPR/Cas9 engineered mammalian cells to induce PTEF-b (CDK9/CT1) knockout in order to study the effect of their depletion on transcription dynamics. Study of the transcription dynamics thanks to fluorescent RNA reporters using MS2/MCP system to determine RNA polymerase elongation rate in living cells.