Experience

Holiferm

• United Kingdom
• Biotechnology Research
• 1 - 100 Employee

• Technical Support Team Lead

  • Aug 2021 - Present

  My current role within Holiferm is support team lead. My current role within Holiferm is support team lead.



Public Health England

• United Kingdom
• Hospitals and Health Care
• 700 & Above Employee

• Healthcare Scientist Support Worker

  • Apr 2021 - Aug 2021

  Working in the virology department based within Manchester royal infirmary my primary role was the receipt and processing of COVID samples from patients. This role required me to adhere to strict protocols of how to handle category 3 biological samples safely and process them efficiently and accurately. I also processed samples using the Biofire and Cepheid rapid turn around testing platforms and performed in house quality control checks on these systems. I also utilised the LIMS system to book samples in for testing. I also helped with more general laboratory tasks such as cleaning and ensuring stocks of consumables and PPE were maintained. Show less



The University of Manchester

• United Kingdom
• Higher Education
• 700 & Above Employee

• Research Technician

  • Aug 2016 - Jun 2020

  Working in the laboratory of Professor Simon Turner within the plant sciences department my role required me to utilise a wide array of techniques. These included techniques such as PCR, recombinant protein expression and purification, genotyping, antibody purification, media preparation, western blotting. histology, plant transformation, microscopy, gas chromatography, tissue culture, various plant husbandry techniques and more. I was also responsible for maintaining consumable stocks and ordering equipment and chemicals and logging all hazardous chemicals using LabCup. I also ensured all relevant risk assessments were in place and wrote methods and helped ensure staff members and students were aware of and adhered to any health and safety requirements and ensured people had undergone any relevant training they needed. Show less

• Research Technician

  • Aug 2013 - Aug 2016

  Working within Manchester fungal infection group (MFIG) my role required me to utilise a wide variety of techniques These included techniques such as cloning, PCR, recombinant protein expression and purification, media preparation, fungal transformation, southern blotting, western blotting, MIC assays, microscopy, fungal culture and more. I was also responsible for maintaining consumable stocks and ordering equipment and chemicals. I was involved in the development of novel experimental techniques and the optimisation of a high throughput gene knockout methodology. I also ensured all relevant health and safety requirements were fulfilled which included writing methods and risk assessments and ensuring people had undergone any relevant training required. Show less

• Research Technician

  • Aug 2010 - Aug 2013

  Working in the laboratory of Professor Simon Turner within the plant sciences department my role required me to utilise a wide array of techniques. These included techniques such as PCR recombinant protein expression and purification, genotyping, antibody purification, western blotting, histology, plant transformation, microscopy, tissue culture, various plant plant husbandry techniques, media preparation and more. I was also responsible for maintaining consumable stocks and ordering equipment and chemicals. I also wrote risk assessments and methods and helped ensure staff members and students were aware of and adhered to any health and safety requirements and ensured people had undergone relevant training. Show less



GENON LABORATORIES LTD

• Mytholmroyd, England, United Kingdom

• Laboratory Manager

  • Dec 2007 - Oct 2009

  My role within Genon laboratories involved the day to day running of a UKAS accredited ISO/IEC 17025:2005 food testing laboratory. My duties included the testing of samples utilising a variety of techniques including ELISA, PCR, real-time PCR and a variety of chemical assays. Responsibilities also included the calibration of equipment, stock maintenance, writing of protocols, development of new techniques including validation, participating in external proficiency rounds and liaising/advising customers. I was involved in the validation and subsequent UKAS accreditation of a number of laboratory techniques including PCR based GMO detection and a number of ELISA based techniques. During my time at Genon I was able to function independently and run a UKAS accredited testing laboratory which entailed liaising with customers, training colleagues, research projects, recording results, issuing certificates, processing, logging and testing of samples and many other duties. Show less



F2G

• United Kingdom
• Biotechnology Research
• 1 - 100 Employee

• Research Assistant

  • Jan 2005 - Dec 2007

  My role within F2G involved the development, running and optimisation of the Mycobank screen, a whole cell genetic screen designed to identify the essential gene set of the filamentous fungus Aspergillus fumigatus. In addition I cloned and expressed/purified proteins identified via Mycobank and was involved in novel assay development relating to these proteins. My position required me to utilise a variety of microbiological and molecular techniques such as fungal/bacterial transformations, PCR, cloning, fungal mutant strain generation including gene KO, Southern blotting, recombinant protein expression and various other techniques. I also researched target genes identified via Mycobank to ascertain their suitability for high throughput screening. Show less

• Laboratory Technician

  • Oct 2003 - Jan 2005

  My initial role at F2G entailed the preparation of media, maintaining supplies of laboratory equipment, waste disposal, environmental monitoring, calibration of laboratory equipment and various other laboratory duties I was also trained in various molecular biology and mycological microbiology techniques.



VITA INDUSTRIAL POLYMERS LIMITED

• United Kingdom

• Assistant Technologist

  • Apr 2000 - Oct 2003

  As an assistant technologist duties included formulation of rubber compounds and preparation of polymer samples. As a quality control technician duties included monitoring of polymers produced on site to ensure strict quality control standards were met. As an assistant technologist duties included formulation of rubber compounds and preparation of polymer samples. As a quality control technician duties included monitoring of polymers produced on site to ensure strict quality control standards were met.



NHS

• Hospitals and Health Care
• 700 & Above Employee

• Trainee MLSO Histology

  • May 1999 - Apr 2000

  Duties included reception and processing of tissue samples utilising histological techniques, patient data entry, waste disposal and the production of various stains and reagents.

• Medical Laboratory Assistant Microbiology

  • Apr 1998 - May 1999

  Duties included preparation of media, waste disposal, patient data entry, reception and processing of various laboratory specimens utilising a variety of microbiological techniques.



The University of Manchester

• United Kingdom
• Higher Education
• 700 & Above Employee

• Laboratory Technician

  • Apr 1997 - Apr 1998

  Based in the Stopford building, duties included preparation of chemicals and equipment for laboratory classes, maintenance and cleaning of laboratory equipment and laying out of equipment for practical classes. Based in the Stopford building, duties included preparation of chemicals and equipment for laboratory classes, maintenance and cleaning of laboratory equipment and laying out of equipment for practical classes.