Experience

23andMe

• United States
• Biotechnology Research
• 400 - 500 Employee

• Director, Functional Genomics

  • Mar 2020 - Present



Amgen

• United States
• Biotechnology Research
• 700 & Above Employee

• Senior scientist

  • Mar 2014 - Mar 2020

  Functional Genomics group leader, Genome Analysis Unit • Perform and direct positive and negative selection pooled CRISPRko, CRISPRi, CRISPRa and shRNA screens for target identification and mechanism of action/resistance studies • Lead development and implementation of a cloning pipeline, reagents and assays for validation of novel T-cell receptors for TCR-T therapeutic candidates • Support siRNA therapeutic development with trigger selection, candidate screening and mechanism of… Show more Functional Genomics group leader, Genome Analysis Unit • Perform and direct positive and negative selection pooled CRISPRko, CRISPRi, CRISPRa and shRNA screens for target identification and mechanism of action/resistance studies • Lead development and implementation of a cloning pipeline, reagents and assays for validation of novel T-cell receptors for TCR-T therapeutic candidates • Support siRNA therapeutic development with trigger selection, candidate screening and mechanism of action studies • Investigate transcriptional and post-translational regulation solutions for cell therapy • Manage viral vector (AAV, LV and HSV) optimization and production

• Scientist

  • May 2011 - Mar 2014

  Target ID & Validation, Genome Analysis Unit • Perform and direct high-throughput, arrayed, cell-based screens for metabolic, inflammation, neuroscience and oncology target and biomarker discovery • Assemble, annotate and maintain an arrayed, cDNA library for overexpression screens



SwitchGear Genomics

• Director of Research and Development

  • Jan 2010 - Apr 2011

  • Develop new promoter and 3’ UTR reporter products • Generate data for marketing literature and product support • Direct sub-cloning of 10,000 reporter clones into a lentiviral vector for a joint venture with Sigma-Aldrich

• Senior Project Manager

  • Jan 2008 - Jan 2010

  • Perform and analyze high-throughput, cell-based promoter and 3’ UTR reporter assays as a functional genomics service for academic and pharma customers • Implement a high-throughput site-directed mutagenesis pipeline for functional validation of transcription factor binding site predictions in cell-based reporter assays • Manage collaborations with academic labs as part of NHGRI’s ENCODE project



Life Technologies

• United States
• Biotechnology Research
• 700 & Above Employee

• Scientist

  • Aug 1999 - Aug 2002

  • Act as lead scientist and project lead for the development of the AmpFlSTR® Identifiler™ PCR Amplification Kit, used for human identity and parentage • Perform test site installs and manage collaborations with federal, state and private crime laboratories • Maintain development documentation for ISO 9001 compliance • Act as lead scientist and project lead for the development of the AmpFlSTR® Identifiler™ PCR Amplification Kit, used for human identity and parentage • Perform test site installs and manage collaborations with federal, state and private crime laboratories • Maintain development documentation for ISO 9001 compliance