eLIFE Ambassador

• Apr 2019 - Present

Scientific Researcher

• Jan 2019 - Present

Postdoctoral Researcher

• Jan 2018 - Present

Postdoctoral Fellow

• Sep 2015 - Oct 2017

Research project: Mechanistic studies of transcription and replication by influenza virus polymerase.Responsible for the safety in the radioactive working room of the institute.Establishment of the technology required to study the enzymatic activity of the influenza viral polymerase in a cell-based system.Independent project and time management, experimental design and execution, data analysis, manuscript writing, presentation of results.Novel technical skills: • Biochemistry: Protein purification (HPLC, size exclusion and affinity chromatography)• Microscopy: Electronic microscopy by negative staining and cryoEM• Molecular biology: Design and performance of enzymologic radioactive assays

Postdoctoral Fellow

• Feb 2011 - Jul 2015

Research project: Role of the nuclear import machinery in influenza virus replication and pathogenesis.Teaching and supervision of graduate, master and PhD students.Supervision of technical staff. Establishment of CRISPR/Cas technology in the laboratoryIndependent project and time management, experimental design and execution, data analysis, manuscript preparation and writing, presentation of results.Novel technical skills: • Animal in vivo experiments: protocol design, animal handling, infection, monitoring and organ excision in BSL2 and BSL3• Virology: production, titration and characterization of recombinant influenza viruses, neuraminidase inhibition assay, hemagglutinin inhibition assay• Production and titration of lentiviral vectors for gene silencing• CRISPR/Cas genome editing techniques for gene silencing in cell lines• Molecular biology: RT-qPCR, real time PCR• Cell biology: Immunofluorescence and flow cytometry.• Microscopy: correlative imaging of total organs to individual cells by using magnetic resonance imaging, fluorescence confocal microscopy, environmental scanning electron microscopy and transmission electron microscopy, optical microscopy. • Bioinformatics: Deep sequencing and analysis of cellular transcriptome and viral genomes

Visiting Postdoctoral Fellow

• Nov 2014 - Dec 2014

Research project: Requirement of the human importin-alpha7 gene during influenza virus polymerase activity in vitro.

Visiting Postdoctoral Fellow

• Sep 2013 - Nov 2013

Research project: Impact of the human importin-alpha7 gene on viral RNA synthesis. Novel technical skills: • Biochemistry: Primer extension assays• Design and performance of radioactive assays

Researcher PHD Student

• 2005 - 2010

Research project: Structural and functional study of the influenza virus polymerase. Supervision of technical staff.Novel technical skills: • Manipulation of cell cultures (mammalian, insect, yeast and bacterial cells).• Molecular biology: Oligonucleotide design, RNA and DNA purification, cloning, sequencing• Biochemistry: Western blot, protein quantification• Electron microscopy by negative staining and protein model reconstruction.• Confocal immunofluorescence microscopy.• Bioinformatics: sequence analysis (ClustalW, Blast), cloning in silico• Design and performance of radioactive assays

Visiting PhD student

• Sep 2009 - Dec 2009

Research project: Study of the assembly of the RNA synthesis machinery of non-segmented negative-strand RNA virusesNovel technical skills: • Biochemistry: Protein and RNA purification (affinity chromatography and sucrose gradient), Western blot, protein quantification, establishment of complex formation assays

Undergraduate Student Researcher

• Jul 2002 - Jul 2005

Research project: Characterization of cellular proteins involved in replication of positive strand RNA virus.

Undergraduate Student Researcher

• Jul 2004 - Mar 2005

Research project: Identification of the human homologue of the yeast decapping activator Pat1 protein.