Experience

H3 Biomedicine

• United States
• Pharmaceutical Manufacturing
• 1 - 100 Employee

• Investigator

  • 2017 - Present

  • Successfully delivered a 3-protein complex enabling downstream HTS, CyroEM and DEL selection.• Optimized protocols for a 4-protein complex of the spliceosome in drug discovery efforts.• Produce multiple antibodies in a campaign for ADC program. Performed large-scale site specific and random bioconjugation to support in vivo pharmacology. Characterized ADCs by HIC and LCMS.• Successfully executed a DEL selection and discovered a hit series with binding at low nM range.• Run LCMS to confirm mass of target protein.• Key support of team members across multiple projects by routinely delivering a range of purified proteins for assays and structural biology.



Syros Pharmaceuticals

• United States
• Pharmaceutical Manufacturing
• 1 - 100 Employee

• Scientist

  • 2015 - 2017

  • Developed protein refolding protocols of transcription factors from inclusion bodies.• Designed and delivered in vivo biontinylated proteins for SPR. • Run Thermal Shift Assay for buffer optimization and confirm compound binding. • Developed protein refolding protocols of transcription factors from inclusion bodies.• Designed and delivered in vivo biontinylated proteins for SPR. • Run Thermal Shift Assay for buffer optimization and confirm compound binding.



Sanofi

• France
• Pharmaceutical Manufacturing
• 700 & Above Employee

• Scientist

  • 2010 - 2015

  • Established protein production capabilities (including bacterial, insect cell, and mammalian cell expression) at a new drug discovery site.• Expressed, purified, and characterized diverse target proteins to enable biochemical (MTS and HTS) and biophysical assays in drug discovery programs.• Developed biochemical assays for small molecule enzyme inhibitor programs including linearity ofenzyme concentration and time, Km substrate determination, DMSO tolerance and Z' calculation.• Miniaturized and automated biochemical assays for IC50 determination.



Novartis Institutes for BioMedical Research (NIBR)

• United States
• Pharmaceutical Manufacturing
• 700 & Above Employee

• Scientist

  • 2007 - 2010

  • Cloned, expressed and purified over 20 diverse proteins for structure-based drug design projects.• Rapidly characterized multiple expression constructs to identify suitable constructs for crystallography.• Initiated crystallization trials and delivered crystals for structure determination with one that enabled a novel crystal structure.• Maintained 6-12 L of insect cell or E.coli cultures for heterologous protein expression.• Provided proteins on 1-200 mg scale for crystallography, biophysical assay and NMR analysis.• Produced biotinylated proteins and seleno-met labeled proteins.• Characterized proteins using DLS, DSLS, and mass spectrometry.



University of Michigan

• United States
• Higher Education
• 700 & Above Employee

• Laboratory Specialist

  • 1995 - 2005

  • Cloned, expressed, and purified untagged cytidylytransferases, ethanolamine kinase, and multiple choline kinase isoforms.• Determined steady-state kinetic parameters for purified enzymes.• Initiated and participated in the de novo structure determination of choline kinase A-2.• Constructed multiple CHO cell lines containing an engineered cytidylyltransferase variant with an altered subcellular localization.• Applied glycerol gradient sedimentation for determining quaternary structure.• Managed a 10-member laboratory.• Established laboratory guidelines for daily operations.• Radiation Officer - maintained laboratory to NRC standards.• Health and Safety Officer - maintained laboratory within OSHA standards.