Oleg Yerov

Associate Scientist at Xilio Therapeutics, Inc.
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(386) 825-5501

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Experience

    • United States
    • Biotechnology Research
    • 1 - 100 Employee
    • Associate Scientist
      • Dec 2021 - 2 years 1 month

      Waltham, Massachusetts, United States

    • Senior Research Associate
      • Sep 2019 - Dec 2021

      Waltham, Massachusetts, United States

    • United States
    • Biotechnology
    • Associate Scientist
      • Feb 2018 - Jul 2019

      1. Lipid Nanoparticle encapsulated mRNA and DNA Gene Therapy for Enzyme Replacement Therapy ● Performed cell and plate-based methods to prove efficacy: ELISA, Enzymatic Activity Assays, Flow Cytometry, Western Blot, RT-PCR and encapsulation assays. ● Cell culture and maintenance of primary cells, immortalized cell lines and differentiating cell lines. ● Transfected multiple cell lines with various transfection reagents. ● In-Vivo testing, collection and sample processing with… Show more 1. Lipid Nanoparticle encapsulated mRNA and DNA Gene Therapy for Enzyme Replacement Therapy ● Performed cell and plate-based methods to prove efficacy: ELISA, Enzymatic Activity Assays, Flow Cytometry, Western Blot, RT-PCR and encapsulation assays. ● Cell culture and maintenance of primary cells, immortalized cell lines and differentiating cell lines. ● Transfected multiple cell lines with various transfection reagents. ● In-Vivo testing, collection and sample processing with wild-type mice. ● Present data to large science team, collaborators, and CROs regularly. Show less

    • United States
    • Biotechnology
    • 1 - 100 Employee
    • Supervisor, Instrument Lab
      • Aug 2016 - Aug 2017

      Lexington, MA 1. Responsible for scheduling and executing studies to submit a Novel Infectious Bacteria detection method to the FDA. 2. Coordinated transfer of materials and samples for a team working 3 shifts a day and 7 days a week. 3. Processed nearly 1000 samples a week regularly while adhering to strict study timelines.

    • Research Associate II
      • Jul 2014 - Aug 2016

      Lexington, MA 1. Developed Novel Hemostasis Assay in whole blood on an innovative instrument. 2. Optimized assay to calculate fibrinogen levels, platelet activity, clot time and blood Lysis levels. 3. Successfully transferred a manual assay to an a semi-automated system. 4. Submitted to the FDA and prepared the system to be sold for research use in Europe.

    • United States
    • Research Services
    • 1 - 100 Employee
    • Research Associate
      • Oct 2013 - Jun 2014

      Boston, MA 1. The Effect of Small molecules on GDNF expression in Mouse Retina- Funded by GlaxoSmithKline Complete regular RT-PCR, qPCR, gel electrophoresis and western blot procedures. Dissect mouse eyes to extract the retina. Perform data analysis to construct new experiments. Received and performed basic animal handling procedures. 2. Cell Therapy with the Differentiation of iPS Cells into eyecups and photoreceptors Established and maintained cell lines using precise aseptic… Show more 1. The Effect of Small molecules on GDNF expression in Mouse Retina- Funded by GlaxoSmithKline Complete regular RT-PCR, qPCR, gel electrophoresis and western blot procedures. Dissect mouse eyes to extract the retina. Perform data analysis to construct new experiments. Received and performed basic animal handling procedures. 2. Cell Therapy with the Differentiation of iPS Cells into eyecups and photoreceptors Established and maintained cell lines using precise aseptic technique. Derive iPS cells from mouse somatic cells. Perform Cryosectioning on eye cups. Perform immunohistochemistry staining on cells and eye cups. Show less

    • Histology Research Associate
      • May 2012 - Jun 2014

      Watertown, MA 1. Stanza® Bioresorbable Scaffold Project ● Dissected sheep tissue including superficial femoral, profunda and internal iliac arteries, pulmonary arteries and renal arteries. ● Performed histological procedures manually, including paraffin sectioning, paraffin embedding, dehydration, and histological stains which include: Hematoxylin and Eosin, Verhoeff Van Gieson’s, Masson’s trichrome, Prussian Blue and Von Kossa’s stain. ● Immunohistochemistry- Smooth Muscle… Show more 1. Stanza® Bioresorbable Scaffold Project ● Dissected sheep tissue including superficial femoral, profunda and internal iliac arteries, pulmonary arteries and renal arteries. ● Performed histological procedures manually, including paraffin sectioning, paraffin embedding, dehydration, and histological stains which include: Hematoxylin and Eosin, Verhoeff Van Gieson’s, Masson’s trichrome, Prussian Blue and Von Kossa’s stain. ● Immunohistochemistry- Smooth Muscle Actin. ● Histopathology scoring for arterial injury, immune response, and scaffold degradation. ● Microscopic imaging of stains and use Photoshop to prepare presentations. ● Histomorphometry measurements using Photoshop and analysis using Excel extensively. ● Presented analysis and conclusions to the core team regularly. 2. DARPA funded ResQFoamTM Project, Arsenal Medical. ● Performed Dissection on Swine tissue including bladder, spleen, liver, peritoneal membrane, bowel, ● Performed Sectioning and Hematoxylin and Eosin staining. Show less

    • United States
    • Medical Equipment Manufacturing
    • 700 & Above Employee
    • Sterility Assurance-Microbiology Intern
      • May 2011 - Jan 2012

      Mahwah, NJ 1. Performed Novel Exhaustive Bioburden Testing and gained experience with protocol revisions. 2. Environmental Monitoring of an ISO 8 cleanroom; Air monitoring, Surface Sampling and Water Testing. 3. Completed weekly cell cultures using aseptic technique.

Education

  • Northeastern University
    Bachelor of Science - BS, Biology/Biological Sciences, General
    2008 - 2013

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