Experience

Virginia State University

• Biomedical Scientist

  • Jun 2019 - Present



University of Karachi, Panjwani Centre for Molecular Drug Discovery

• Visiting Faculty

  • Aug 2014 - Present

  -Responsibilities include research on biologically active ingredients, purification andcharacterization of biologically active fractions from various medicinal plants andconsumable natural products.-Currently working on purification and characterization of proteins and peptides toevaluate their biological role in diabetes, atherosclerosis and cancer (in collaboration withother specialized groups). Also conducting projects related to genetic mutations invarious enzyme… -Responsibilities include research on biologically active ingredients, purification andcharacterization of biologically active fractions from various medicinal plants andconsumable natural products.-Currently working on purification and characterization of proteins and peptides toevaluate their biological role in diabetes, atherosclerosis and cancer (in collaboration withother specialized groups). Also conducting projects related to genetic mutations invarious enzyme families.-Teach courses in macromolecule purification and characterization and on special topics inprotein chemistry.-Conduct workshops on DNA sequencing, protein analysis, gene-cloning and expression(including lectures and hands-on lab exercises for students and researchers).-Manage cell culture facility, analytical laboratory and operation and upgrades ofspecialized instruments. Show more Show less



Next Generation Sequencing company

• Independent Sales Representative

  • Jun 2013 - Oct 2013

  Independent Sales Manager- June-2013Independent Sales Manager for an expanding Next generation Sequencing/ Biotech company.-Company confidential:Duties include:Responsible for increasing sales volume and informing customers of the advantages of highly efficient kits used for latest sequencing technology, especially in core facility setting. These are multiple products from a very reliable company and are destined to make a great difference in the productivity and cost… Independent Sales Manager- June-2013Independent Sales Manager for an expanding Next generation Sequencing/ Biotech company.-Company confidential:Duties include:Responsible for increasing sales volume and informing customers of the advantages of highly efficient kits used for latest sequencing technology, especially in core facility setting. These are multiple products from a very reliable company and are destined to make a great difference in the productivity and cost effectiveness of customers who choose to use them in their research projects and work in core facility setting. Duties include mailing Potential customers with product information, answering technical questions and offering solutions etc., visiting customer sites, making phone calls to check on customer orders and quotes, coordinate quotes and ordering information, follow-up with customers, attend vendor shows, distribute company’s product literature and in formats convenient to customers. Show more Show less



FDA

• Chemist

  • Mar 2004 - Apr 2012

  Started and established full service DNA sequencing facility in CBER, FDA. Managed and conducted all aspect of services and provided efficient and cost effective services to hundreds of scientists. Also responsible for continuous upgrade of instruments, training of technical personnel and making sure that all services were completed to user satisfaction.Responsibilities included DNA sequencing services and project support in DNA sequencing related services to scientists working at FDA and… Started and established full service DNA sequencing facility in CBER, FDA. Managed and conducted all aspect of services and provided efficient and cost effective services to hundreds of scientists. Also responsible for continuous upgrade of instruments, training of technical personnel and making sure that all services were completed to user satisfaction.Responsibilities included DNA sequencing services and project support in DNA sequencing related services to scientists working at FDA and NIH. Hands-on experience with variety of Genetic Analyzers/DNA sequencers and accessory equipment including: 3500xl, 3130xl, 3100, and 310 instruments and in performing complete processing and analysis of samples• Provided timely and cost effective services for DNA sequencing, fragment analysis (genotyping), and small scale sequencing projects• Provided special consideration to high priority projects when requested by user(s).• Analyzed and submitted sequencing data to researchers and provided consultation to users for project and data management, troubleshooting any problems related to specific samples on a case-by-case basis. Conducted a follow-up on all samples and projects• Backed up all DNA sequencing and genotyping data locally for any future retrieval. • Responsible for smooth and sustainable operation of the DNA sequencing instruments, service calls, and on-line off-line trouble-shooting• Generated $1.6 million in revenue since March 2004 and analyzed over 250,000 samples• Full cost recovery for all service aspects, instruments purchase, upgrade(s), service contracts and supplies Show more Show less



University of Maryland, Baltimore, MD

• Manager of BioAnalytical Core Facility. Center of Marine Biotechnology

  • Feb 1999 - Mar 2004

  Facility provided services to in-house researchers as well as outside scientists.· Maintained and operated the following services and instruments:- Automated DNA Sequencing using ABI instrumentation DNA Sequencer-377, 373xl, and 310- Oligo-synthesis on Beckman-1000 synthesizer (regular and labeled probes)- Small Scale DNA and EST sequencing projects and fragment analysis using ABI sequencers(310, 373xl and 377) - Gene quantitation using TaqMan (ABI-7700)- Protein… Facility provided services to in-house researchers as well as outside scientists.· Maintained and operated the following services and instruments:- Automated DNA Sequencing using ABI instrumentation DNA Sequencer-377, 373xl, and 310- Oligo-synthesis on Beckman-1000 synthesizer (regular and labeled probes)- Small Scale DNA and EST sequencing projects and fragment analysis using ABI sequencers(310, 373xl and 377) - Gene quantitation using TaqMan (ABI-7700)- Protein sequencing from PVDF blotted and purified or isolated and eluted fractionsusing Beckman Gas-Phase sequencer (LG3000)· Gel-imaging for regular gels for DNA analysis using Storm-Imager· Purchased equipment to enhance capability by contributing to grants· Maintained quality and self-sufficiency in operating costs (personnel and operational).Completion of orders for filing and reimbursement· Generated $500,000 in revenue since Feb. 1999 and analyzed 50,000 samples for DNAsequencing while performing other services. Full cost recovery for independentlysupporting core operation. Show more Show less



Meharry Medical College

• Associate Director and Director Molecular Biology Core Facility

  • Jan 1991 - Jan 1999

  Managed DNA and protein core facility in DNA sequencing and synthesis, proteinsequencing and purification, peptide synthesis, gel-imaging, and PCR (The core facilityprovided services in these techniques to users at the medical school.)· Conducted research projects with funding from AHA and NIH on heparin and bindingsynthetic peptides from lipoprotein lipase (grants funded from AHA, 1997 to 1999. Sequencetag-analysis from various parasitic organisms, such as T. brucei (some… Managed DNA and protein core facility in DNA sequencing and synthesis, proteinsequencing and purification, peptide synthesis, gel-imaging, and PCR (The core facilityprovided services in these techniques to users at the medical school.)· Conducted research projects with funding from AHA and NIH on heparin and bindingsynthetic peptides from lipoprotein lipase (grants funded from AHA, 1997 to 1999. Sequencetag-analysis from various parasitic organisms, such as T. brucei (some clones submitted toNCBI-data base)· Conducted and implemented protocols for cost efficiency and rapid turnaround time, anddeveloped methods for template purification for DNA sequencing and protein sequenceanalysis· Completed grant packages to avail instrumentation grants from NIH and NSF. Costrecovery and personal cost recovery through grants.· Instructor on basic analytical courses for graduate students. Show more Show less



Fogarty Visiting Fellowship

• Postdoctoral Fellow

  • Jun 1988 - Jan 1991

  Worked as a postdoctoral fellow on a project to analyze lipoprotein lipase (LPL) at themolecular level with patients that had a deficiency of the enzyme· Conducted detailed analysis of the enzyme in different patients· Conducted first detailed analyses of the structure-function of LPL using site directedmutagenesis of LPL gene· Made routine use of the following techniques: DNA sequencing, oligo synthesis, genecloning, gene expression, protein sequencing, protein… Worked as a postdoctoral fellow on a project to analyze lipoprotein lipase (LPL) at themolecular level with patients that had a deficiency of the enzyme· Conducted detailed analysis of the enzyme in different patients· Conducted first detailed analyses of the structure-function of LPL using site directedmutagenesis of LPL gene· Made routine use of the following techniques: DNA sequencing, oligo synthesis, genecloning, gene expression, protein sequencing, protein expression and protein purification · Completed papers for publication in scientific journals and presentation(s) in various scientific meetings Show more Show less



John Curtin School of Medical Research

• Postdoctoral Fellow

  • Aug 1987 - May 1988

  Worked on structure elucidation of rabbit transferring and various milk proteins frommotremes and marsupials.· Operated state-of-the-art instruments used in protein sequence analysis and proteinpeptide purification. · Published results in scientific journals.