Experience

PopVax

• India
• Biotechnology Research
• 1 - 100 Employee

• Formulation Scientist

  • Oct 2022 - Present

  Major responsibilities: Developing thermostable, chimeric, low-cost, and safe second-generation mRNA-loaded lipid nanoparticle vaccines to combat current and future strains of SARS-CoV-2. Physical characterization (Dynamic Light Scattering, Electrophoretic Light Scattering), determining encapsulation efficiency (RiboGreen assay and related) and in vitro functional characterization (Transfection and protein expression) of lipid nanoparticles. Thermostability and shelf-life studies of lipid nanoparticles. Minor responsibilities: Media development, 2D culture and maintenance of mammalian cells. Show less



The Hebrew University of Jerusalem

• Israel
• Higher Education
• 700 & Above Employee

• Research Associate

  • Oct 2021 - Sep 2022

  Major responsibilities:Tracking the dynamics of metabolic changes driving early human development on chip. Media development for human pluripotent stem cells (hPSCs; naïve and primed), and spontaneously differentiating embryoid bodies. 2D and 3D hPSCs culture and maintenance. Immunocytochemistry. Confocal microscopy and image analysis. Quantitative RT-PCR. Microfabrication of organ-on-a-chip systems using various microfabrication techniques. Bioinstrumentation and automation of organ-on-a-chip systems. Real-time measurement of oxygen and metabolites (glucose, lactate, glutamine) using microsensors and electrochemical sensors, respectively. Metabolic flux analysis. Studying the effects of drugs on metabolism and circadian rhythms in developing embryoid bodies on chip.Minor responsibilities:Media development and differentiating primed hPSCs into other cell types (mainly cardiomyocytes and hepatocytes). 2D and 3D culture and maintenance of stem cell derived-cardiomyocytes, primary liver and kidney cells. Show less

• Master's Student

  • Mar 2019 - Feb 2022

• Research Assistant

  • Oct 2018 - Sep 2021

  Major responsibilities:Tracking the dynamics of metabolic changes driving early human development on chip. Media development for human pluripotent stem cells (hPSCs; naïve and primed), and spontaneously differentiating embryoid bodies. 2D and 3D hPSCs culture and maintenance. Immunocytochemistry. Confocal microscopy and image analysis. Quantitative RT-PCR. Microfabrication of organ-on-a-chip systems using various microfabrication techniques. Bioinstrumentation and automation of organ-on-a-chip systems. Real-time measurement of oxygen and metabolites (glucose, lactate, glutamine) using microsensors and electrochemical sensors, respectively. Metabolic flux analysis. Studying the effects of drugs on metabolism and circadian rhythms in developing embryoid bodies on chip.Minor responsibilities:Media development and differentiating primed hPSCs into other cell types (mainly cardiomyocytes and hepatocytes). 2D and 3D culture and maintenance of stem cell derived-cardiomyocytes, primary liver and kidney cells. Show less



Indian Institute of Science (IISc)

• India
• Research
• 700 & Above Employee

• Research Assistant

  • Jan 2017 - Sep 2018

  Major responsibilities: Microfluidic device designing, simulations, fabrication, biofunctionalization, validation, and optimization. Conjugation of biomolecules (nucleotides and proteins) on hydrophobic polymeric surfaces (mainly Polydimethylsiloxane) and metallic nanoparticles (mainly Superparamagnetic iron oxide). Minor responsibilities: Assisting lab members and visiting members mainly with biological wet lab work (cell culture and molecular biology techniques for optofluidics) and occasionally with some microfabrication techniques (photolithography and soft lithography). Show less