Senior Scientist

• Jun 2022 - Present

Scientific Investigator

• Apr 2020 - Mar 2022

Engineer II, Downstream Process Development

• Jun 2017 - Apr 2020

Research Associate

• Jan 2017 - Jun 2017

Research Associate, Antibody and Protein Production Member of the Protein Science team involved in:  Managing transient transfection platform to support the discovery pipeline weekly involving 10 to 12 L cell culture (HEK cells), maintaining parental cell seed train, plasmid amplification and purification, transient transfection and filtration of harvested supernatant.  Maintaining detailed records of experimental protocols and data in laboratory notebooks in accordance with company… Show more Research Associate, Antibody and Protein Production Member of the Protein Science team involved in:  Managing transient transfection platform to support the discovery pipeline weekly involving 10 to 12 L cell culture (HEK cells), maintaining parental cell seed train, plasmid amplification and purification, transient transfection and filtration of harvested supernatant.  Maintaining detailed records of experimental protocols and data in laboratory notebooks in accordance with company policy. Consistently deliver results meeting project deadlines.  Analysis, interpretation and reporting experimental results in a small group setting. Show less Research Associate, Antibody and Protein Production Member of the Protein Science team involved in:  Managing transient transfection platform to support the discovery pipeline weekly involving 10 to 12 L cell culture (HEK cells), maintaining parental cell seed train, plasmid amplification and purification, transient transfection and filtration of harvested supernatant.  Maintaining detailed records of experimental protocols and data in laboratory notebooks in accordance with company… Show more Research Associate, Antibody and Protein Production Member of the Protein Science team involved in:  Managing transient transfection platform to support the discovery pipeline weekly involving 10 to 12 L cell culture (HEK cells), maintaining parental cell seed train, plasmid amplification and purification, transient transfection and filtration of harvested supernatant.  Maintaining detailed records of experimental protocols and data in laboratory notebooks in accordance with company policy. Consistently deliver results meeting project deadlines.  Analysis, interpretation and reporting experimental results in a small group setting. Show less

Laboratory Technician

• Apr 2013 - Nov 2013

- Performed Hydrophobic Interaction chromatography (HIC) on AKTA Pilot and Avant, Immobilized metal ion affinity chromatography (IMAC) on AKTA purifier, Anion Exchange (ANX) and Cation Exchange (CNX) chromatography on AKTA purifier, Ultra filtration, Depth filtration in the Purification Department of MSAT laboratory. - Writing Methods, evaluating the chromatograms, generating reports using Unicorn Software for AKTA Purifiers, AKTA Pilot and Avant. - Performed Hydrophobic Interaction chromatography (HIC) on AKTA Pilot and Avant, Immobilized metal ion affinity chromatography (IMAC) on AKTA purifier, Anion Exchange (ANX) and Cation Exchange (CNX) chromatography on AKTA purifier, Ultra filtration, Depth filtration in the Purification Department of MSAT laboratory. - Writing Methods, evaluating the chromatograms, generating reports using Unicorn Software for AKTA Purifiers, AKTA Pilot and Avant.

Researcher

• Aug 2011 - Dec 2012

• Conventional cell culture methods involving passage maintenance, scheduled media changes and cryogenic preservation of cells. Experienced in 2D culture of primary human cells (keratinocytes, bone marrow and hair follicle stem cells) and cell lines (epithelial carcinoma A431, HEK-293T). • Performing western blots, immunocytochemistry and flow-cytometry based protein evaluation for studying effects on gene expression post lentiviral transduction. • Lentiviral vector development for… Show more • Conventional cell culture methods involving passage maintenance, scheduled media changes and cryogenic preservation of cells. Experienced in 2D culture of primary human cells (keratinocytes, bone marrow and hair follicle stem cells) and cell lines (epithelial carcinoma A431, HEK-293T). • Performing western blots, immunocytochemistry and flow-cytometry based protein evaluation for studying effects on gene expression post lentiviral transduction. • Lentiviral vector development for studies in the laboratory involving gene over expression, shRNA based gene silencing, dominant negative expression and promoter region replacements or modifications in viral vectors. • Adept in standard cloning techniques comprising of primer design, running PCR reactions, restriction digestion, gel and PCR purification, competent cell transformation, molecular screening procedures etc . • Plasmid purification from bacterial cultures in Mini, Midi and Maxi-prep formats followed by quality tests. • Experienced in lentivirus production methods involving plasmid transfections into 293T cells followed by vector purification of viral supernatants by high speed centrifugation and titration assays for transduction efficiency. • Selection of high expression clones by developing ‘kill curve’ assays using serial dilutions of chemical selection reagents such as puromycin, hygromycin etc. • Assisting in carrying out differentiation protocols for mesenchymal stem cell (MSCs) to osteogenic, chondrogenic and adipogenic lineages. This involves media formulation and optimization studying the effects of differentiation components on expression of lineage specific markers. Show less • Conventional cell culture methods involving passage maintenance, scheduled media changes and cryogenic preservation of cells. Experienced in 2D culture of primary human cells (keratinocytes, bone marrow and hair follicle stem cells) and cell lines (epithelial carcinoma A431, HEK-293T). • Performing western blots, immunocytochemistry and flow-cytometry based protein evaluation for studying effects on gene expression post lentiviral transduction. • Lentiviral vector development for… Show more • Conventional cell culture methods involving passage maintenance, scheduled media changes and cryogenic preservation of cells. Experienced in 2D culture of primary human cells (keratinocytes, bone marrow and hair follicle stem cells) and cell lines (epithelial carcinoma A431, HEK-293T). • Performing western blots, immunocytochemistry and flow-cytometry based protein evaluation for studying effects on gene expression post lentiviral transduction. • Lentiviral vector development for studies in the laboratory involving gene over expression, shRNA based gene silencing, dominant negative expression and promoter region replacements or modifications in viral vectors. • Adept in standard cloning techniques comprising of primer design, running PCR reactions, restriction digestion, gel and PCR purification, competent cell transformation, molecular screening procedures etc . • Plasmid purification from bacterial cultures in Mini, Midi and Maxi-prep formats followed by quality tests. • Experienced in lentivirus production methods involving plasmid transfections into 293T cells followed by vector purification of viral supernatants by high speed centrifugation and titration assays for transduction efficiency. • Selection of high expression clones by developing ‘kill curve’ assays using serial dilutions of chemical selection reagents such as puromycin, hygromycin etc. • Assisting in carrying out differentiation protocols for mesenchymal stem cell (MSCs) to osteogenic, chondrogenic and adipogenic lineages. This involves media formulation and optimization studying the effects of differentiation components on expression of lineage specific markers. Show less

Bioprocess Engineering Intern

• Feb 2011 - May 2011

Scale up study of expression of human proteins in algal host cells. • The expression system with a photo-inducible promoter was used for protein synthesis. Different light induction strategies were employed to optimize the protein yield. Performance comparison of strains from different sources and their clonal selection for protein synthesis was carried out. • Monitoring fermentation metrics in terms of growth kinetics (OD measurements) and product yield during various stages of the… Show more Scale up study of expression of human proteins in algal host cells. • The expression system with a photo-inducible promoter was used for protein synthesis. Different light induction strategies were employed to optimize the protein yield. Performance comparison of strains from different sources and their clonal selection for protein synthesis was carried out. • Monitoring fermentation metrics in terms of growth kinetics (OD measurements) and product yield during various stages of the scale up. • Scale up culture volumes from 100 ml, 250 ml shake flasks to 8 and 36 lit algal bioreactors. • Prepared optimized media formulations, culture plates for different algal strains. Carried out sterilization procedures for instruments and media, calibration and troubleshooting of instruments. Show less Scale up study of expression of human proteins in algal host cells. • The expression system with a photo-inducible promoter was used for protein synthesis. Different light induction strategies were employed to optimize the protein yield. Performance comparison of strains from different sources and their clonal selection for protein synthesis was carried out. • Monitoring fermentation metrics in terms of growth kinetics (OD measurements) and product yield during various stages of the… Show more Scale up study of expression of human proteins in algal host cells. • The expression system with a photo-inducible promoter was used for protein synthesis. Different light induction strategies were employed to optimize the protein yield. Performance comparison of strains from different sources and their clonal selection for protein synthesis was carried out. • Monitoring fermentation metrics in terms of growth kinetics (OD measurements) and product yield during various stages of the scale up. • Scale up culture volumes from 100 ml, 250 ml shake flasks to 8 and 36 lit algal bioreactors. • Prepared optimized media formulations, culture plates for different algal strains. Carried out sterilization procedures for instruments and media, calibration and troubleshooting of instruments. Show less

Teaching assistant

• 2008 - 2009

Process Engineering Consultant

• Aug 2007 - Jul 2008

• Consulting team member on multiple pharmaceutical industry projects for Merck India Ltd., Ajanta Pharma Ltd. and Kumar Organic Products Ltd. where I got acquainted to industry standards and practices. • Prepared the Quotation Comparison statements for the various chemical engineering equipments valued about $20000 and more. • Prepared the process diagrams from the process description of the new chemical products of the client. • Carried out material and energy balance calculations… Show more • Consulting team member on multiple pharmaceutical industry projects for Merck India Ltd., Ajanta Pharma Ltd. and Kumar Organic Products Ltd. where I got acquainted to industry standards and practices. • Prepared the Quotation Comparison statements for the various chemical engineering equipments valued about $20000 and more. • Prepared the process diagrams from the process description of the new chemical products of the client. • Carried out material and energy balance calculations incorporating the raw batch data to prepare the Process Flow Diagrams. Also performed hydraulic calculations for piping layout. • Maintained the piping line lists and completed the datasheets of the process equipments for subsequent use by mechanical engineers. Documented the process by keeping record for line lists, inventory list which are required to consolidate the cost estimates. • Collaborated with the draftsmen to create and revise the instrumentations and process control plans aka P&IDs on AutoCAD. • Responsible for preparing the SOPs and qualifications for installment, operation and performance IQ, OQ, PQ for the process equipment. Show less • Consulting team member on multiple pharmaceutical industry projects for Merck India Ltd., Ajanta Pharma Ltd. and Kumar Organic Products Ltd. where I got acquainted to industry standards and practices. • Prepared the Quotation Comparison statements for the various chemical engineering equipments valued about $20000 and more. • Prepared the process diagrams from the process description of the new chemical products of the client. • Carried out material and energy balance calculations… Show more • Consulting team member on multiple pharmaceutical industry projects for Merck India Ltd., Ajanta Pharma Ltd. and Kumar Organic Products Ltd. where I got acquainted to industry standards and practices. • Prepared the Quotation Comparison statements for the various chemical engineering equipments valued about $20000 and more. • Prepared the process diagrams from the process description of the new chemical products of the client. • Carried out material and energy balance calculations incorporating the raw batch data to prepare the Process Flow Diagrams. Also performed hydraulic calculations for piping layout. • Maintained the piping line lists and completed the datasheets of the process equipments for subsequent use by mechanical engineers. Documented the process by keeping record for line lists, inventory list which are required to consolidate the cost estimates. • Collaborated with the draftsmen to create and revise the instrumentations and process control plans aka P&IDs on AutoCAD. • Responsible for preparing the SOPs and qualifications for installment, operation and performance IQ, OQ, PQ for the process equipment. Show less