Experience

Cadila Healthcare Limited, Ahmedabad

• India
• Biotechnology Research
• 1 - 100 Employee

• Assistant Manager

  • Mar 2023 - Present

• Senior Executive

  • Mar 2021 - Mar 2023

• Executive

  • Jul 2015 - Mar 2021

  • Working as Executive - Production, in Downstream processing dept.of Zydus Biologics unit 1, unit of Cadila Healthcare Ltd, Ahmedabad, Gujarat.Experience and Responsibilities:• Purification of recombinant therapeutic molecules such as GCSF, PEG GCSF, PTH, IFN, IFN, PEG-IFN and EPO• Execution of purification activities involving centrifugation, chromatography steps, TFF and filtration steps starting from solubilisation/ Refolding of inclusion bodies to preparation of Drug substance. • Qualification and Performance verification activities of downstream related equipment such as AKTA Pilot, AKTA Process, autoclave and DHS unit.• Responsible for monitoring of filter integrity related activity for DSP.• Cleaning validation (CV) of glassware, SS wares, Reaction vessels, TFF, columns and AKTA systems.• SAP related activities. Show less



Symbiotec Pharmalab Limited

• Junior Executive in Biotech – Downstream dept

  • Oct 2014 - Jun 2015

  Experience and Responsibilities: • Handling the batch process of recovery and purification of RM for steroid & hormones in a DCS operated API plant. Recovery via Solvent extraction and Westfalia Decanter separation and its purification by (i) subsequent distillation and (ii) filtration and drying via Agitated Nutsche Filter Dryer (ANFD); the whole process in multiple steps (3), using different solvents at each subsequent step. • Learned and performed calibration of reactor tanks by checking their mm H2O, DL & LT% measurements. • Initially traced solvent lines in / out of reactor with hot water followed by hot caustic cleaning and flushing of solvent lines and reactor tanks. Achievements: • Learning the Operation of (1) ANFD – Agitated Nutshe Filter Dryer (Make – Bew Engineering, Mumbai) (2) Westfalia Decanter – for separation of broth and Rich Solvent. Show less



Rashi Biotech Pvt Ltd

• Production Officer

  • May 2013 - Oct 2014

  • Worked at Rashi Biotech Pvt. Ltd. (API), Uruli Kanchan in Pune as Production Officer in Downstream & Upstream Processing from May 2013 to present. • Lead a group of 3 officers & 4 assistants. Experience & Responsibilities: 1. Downstream processing of culture medium : • Micro filtration, Ultra filtration using hollow fiber & TFF membrane. • Chromatography Column handling & its packing. • Protein purification 1. Ammonium Sulphate precipitation & Dialysis. 2. Ion exchange chromatography & Gel filtration. • Preparation of buffers for chromatography & SDS PAGE. QC (Downstream): 1. SDS PAGE. 2. Agar well Diffusion assay – GCZ, casein assay. 2. Upstream processing of culture medium : • Preparation, Sterilization & inoculation of seed & production medium. • Sterilization of medium & glassware’s. • Preservation & Maintenance of bacterial culture. • Operated 2700 lit bioreactor (Hexamide Agrotech) Started from 100 ml seed & up scaled it up to 400 lit production trial batches in 4 steps. QC (Upstream): 1. Pour Plate, Sterility check of sterilized media & culture samples. 2. Gram Stain & microscopy. 3. Agar well Diffusion assay – GCZ, casein assay. 4. Environmental monitoring via settle plate, contact plate & swabbing method. Achievements: • Studied & learned the Operation of DM plant & its regeneration. • Studied & learned the operation of Tubular Centrifuge (Make – Sharplex Filters (India) Pvt. Ltd.) • Studied & learned the Operation of octagonal blender (Make-Vitro Pharma Instrument) & shifter (Make-vitro pharma instrument). (for formulation) Show less



A J Organica Pvt Ltd

• Project Research Associate

  • Feb 2012 - Apr 2013

  • Isolation and purification of natural organic compounds by Gel filtration, Affinity chromatography, Adsorption chromatography and MPLC. • Work directed at carrying out protein binding assay to identify the compound of interest from the mixture of natural organic molecules and subsequent separation by TLC. • Small molecular interaction with protein immobilized on affinity matrix. (Project) Small molecular interaction of target molecule with a specific protein immobilized on a specific functional group based affinity matrix aiding its one step purification. Achievements: • Helped develop and design reverse phase chromatography (RPC) based MPLC system. • Directed working of 10 lit, 20 lit and 50 lit vacuum distillation assemblies in the company. • Helped develop and design simple domestic chilling system for rotary evaporators. Show less



Niramaya Hospital, Chinchwad.

• Industrial Training

  • May 2004 - Jun 2004

  1 month. 14th May 2006 to 14th June. Institute: Fergusson College, Pune Industry: Nirmaya hospital. Job Description: To get trained to work with the apparatus such ELISA, HPLC and other instruments. Got the knowledge & practically handled various instruments used in pathological lab. ANOTHER TRAINING PERIOD in same hospital from 27th june 2006 to 27th july 2006 1 month. 14th May 2006 to 14th June. Institute: Fergusson College, Pune Industry: Nirmaya hospital. Job Description: To get trained to work with the apparatus such ELISA, HPLC and other instruments. Got the knowledge & practically handled various instruments used in pathological lab. ANOTHER TRAINING PERIOD in same hospital from 27th june 2006 to 27th july 2006