Nick Fredette
Scientist at Aquinnah Pharmaceuticals- Claim this Profile
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English Native or bilingual proficiency
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Armenian Full professional proficiency
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Spanish Elementary proficiency
Topline Score
Bio
Experience
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Aquinnah Pharmaceuticals
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United States
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Biotechnology Research
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1 - 100 Employee
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Scientist
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Feb 2021 - Present
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Karyopharm Therapeutics Inc.
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United States
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Pharmaceutical Manufacturing
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200 - 300 Employee
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Associate Scientist
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Jan 2020 - Feb 2021
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Aquinnah Pharmaceuticals
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United States
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Biotechnology Research
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1 - 100 Employee
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Research Associate II
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Oct 2017 - Jan 2020
• Independently led screening program involving weekly design, assay execution, analysis, and presentation of results • Optimized cell-based assay for high-throughput screen – increased weekly screening capacity 50-fold through iterative troubleshooting • Developed robust secondary assay platform to characterize compounds from HTS • Implemented a standardized compound receival, registration, and storage system • Harvested, plated and maintained rodent primary neurons • Independently led screening program involving weekly design, assay execution, analysis, and presentation of results • Optimized cell-based assay for high-throughput screen – increased weekly screening capacity 50-fold through iterative troubleshooting • Developed robust secondary assay platform to characterize compounds from HTS • Implemented a standardized compound receival, registration, and storage system • Harvested, plated and maintained rodent primary neurons
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Beth Israel Deaconess Medical Center
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United States
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Hospitals and Health Care
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700 & Above Employee
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Research Assistant II
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Jul 2015 - Sep 2017
• Setup and harvested TZM.bl neutralization assays using a wide variety of sample types • Carried out PBMC isolation from whole blood samples using Ficoll-Paque method on a daily basis • Transfected 293T/17 cells to produce pseudovirus and carried out corresponding TCID50 assays • Maintained PBMC sample database and prepared international shipments through World Courier • Processed vaginal mucus, semen, saliva, and rectal samples for an assortment of clinical studies • Received Good Clinical Lab Practice training and certification • Experienced in handling, shipping and processing HIV+ whole blood samples Show less
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Ironwood Pharmaceuticals
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United States
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Biotechnology Research
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200 - 300 Employee
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Research Intern
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May 2014 - Aug 2014
• Performed numerous in vitro P-glycoprotein permeability assays using canine MDCK-MDR1 cells • Maintained, plated, and transferred MDCK-MDR1 cells daily for DMPK • Presented permeability data weekly at department meetings • Provided structure-activity relationships (SAR) data for the Department of Medicinal Chemistry • Worked extensively with Mass Spectrometry instruments and evaluated corresponding data • Performed numerous in vitro P-glycoprotein permeability assays using canine MDCK-MDR1 cells • Maintained, plated, and transferred MDCK-MDR1 cells daily for DMPK • Presented permeability data weekly at department meetings • Provided structure-activity relationships (SAR) data for the Department of Medicinal Chemistry • Worked extensively with Mass Spectrometry instruments and evaluated corresponding data
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Wheaton College
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United States
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Higher Education
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700 & Above Employee
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Undergraduate Research Assistant
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Jun 2013 - Jul 2013
• Handled and homogenized brain and liver samples of mice treated with methylmercury • Examined effect of methylmercury on enzyme level variations in mice • Carried out assays targeting enzyme levels of the brain and liver with the use of spectrophotometry • Used Excel spreadsheets to calculate protein levels in mice brain and liver tissues from assay results • Handled and homogenized brain and liver samples of mice treated with methylmercury • Examined effect of methylmercury on enzyme level variations in mice • Carried out assays targeting enzyme levels of the brain and liver with the use of spectrophotometry • Used Excel spreadsheets to calculate protein levels in mice brain and liver tissues from assay results
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Dowd Medical
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Reading, MA
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Migration Task Force Member
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Jun 2012 - Aug 2012
• Migrated patient list of Dr. Howard Rashba into an electronic medical record utilizing eClinical works • Observed daily patient check-ups, vaccinations and testing procedures for multiple infectious diseases • Exposed to appointments with patients having special needs and learning disabilities • Migrated patient list of Dr. Howard Rashba into an electronic medical record utilizing eClinical works • Observed daily patient check-ups, vaccinations and testing procedures for multiple infectious diseases • Exposed to appointments with patients having special needs and learning disabilities
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Education
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Wheaton College (MA)
Bachelor of Arts (B.A.), Chemistry -
Stoneham High School
High School Diploma