Nick Fredette

Scientist at Aquinnah Pharmaceuticals
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Contact Information
us****@****om
(386) 825-5501
Location
Cambridge, Massachusetts, United States, US
Languages
  • English Native or bilingual proficiency
  • Armenian Full professional proficiency
  • Spanish Elementary proficiency

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Experience

    • United States
    • Biotechnology Research
    • 1 - 100 Employee
    • Scientist
      • Feb 2021 - Present

    • United States
    • Pharmaceutical Manufacturing
    • 200 - 300 Employee
    • Associate Scientist
      • Jan 2020 - Feb 2021

    • United States
    • Biotechnology Research
    • 1 - 100 Employee
    • Research Associate II
      • Oct 2017 - Jan 2020

      • Independently led screening program involving weekly design, assay execution, analysis, and presentation of results • Optimized cell-based assay for high-throughput screen – increased weekly screening capacity 50-fold through iterative troubleshooting • Developed robust secondary assay platform to characterize compounds from HTS • Implemented a standardized compound receival, registration, and storage system • Harvested, plated and maintained rodent primary neurons • Independently led screening program involving weekly design, assay execution, analysis, and presentation of results • Optimized cell-based assay for high-throughput screen – increased weekly screening capacity 50-fold through iterative troubleshooting • Developed robust secondary assay platform to characterize compounds from HTS • Implemented a standardized compound receival, registration, and storage system • Harvested, plated and maintained rodent primary neurons

    • United States
    • Hospitals and Health Care
    • 700 & Above Employee
    • Research Assistant II
      • Jul 2015 - Sep 2017

      • Setup and harvested TZM.bl neutralization assays using a wide variety of sample types • Carried out PBMC isolation from whole blood samples using Ficoll-Paque method on a daily basis • Transfected 293T/17 cells to produce pseudovirus and carried out corresponding TCID50 assays • Maintained PBMC sample database and prepared international shipments through World Courier • Processed vaginal mucus, semen, saliva, and rectal samples for an assortment of clinical studies • Received Good Clinical Lab Practice training and certification • Experienced in handling, shipping and processing HIV+ whole blood samples Show less

    • United States
    • Biotechnology Research
    • 200 - 300 Employee
    • Research Intern
      • May 2014 - Aug 2014

      • Performed numerous in vitro P-glycoprotein permeability assays using canine MDCK-MDR1 cells • Maintained, plated, and transferred MDCK-MDR1 cells daily for DMPK • Presented permeability data weekly at department meetings • Provided structure-activity relationships (SAR) data for the Department of Medicinal Chemistry • Worked extensively with Mass Spectrometry instruments and evaluated corresponding data • Performed numerous in vitro P-glycoprotein permeability assays using canine MDCK-MDR1 cells • Maintained, plated, and transferred MDCK-MDR1 cells daily for DMPK • Presented permeability data weekly at department meetings • Provided structure-activity relationships (SAR) data for the Department of Medicinal Chemistry • Worked extensively with Mass Spectrometry instruments and evaluated corresponding data

    • United States
    • Higher Education
    • 700 & Above Employee
    • Undergraduate Research Assistant
      • Jun 2013 - Jul 2013

      • Handled and homogenized brain and liver samples of mice treated with methylmercury • Examined effect of methylmercury on enzyme level variations in mice • Carried out assays targeting enzyme levels of the brain and liver with the use of spectrophotometry • Used Excel spreadsheets to calculate protein levels in mice brain and liver tissues from assay results • Handled and homogenized brain and liver samples of mice treated with methylmercury • Examined effect of methylmercury on enzyme level variations in mice • Carried out assays targeting enzyme levels of the brain and liver with the use of spectrophotometry • Used Excel spreadsheets to calculate protein levels in mice brain and liver tissues from assay results

  • Dowd Medical
    • Reading, MA
    • Migration Task Force Member
      • Jun 2012 - Aug 2012

      • Migrated patient list of Dr. Howard Rashba into an electronic medical record utilizing eClinical works • Observed daily patient check-ups, vaccinations and testing procedures for multiple infectious diseases • Exposed to appointments with patients having special needs and learning disabilities • Migrated patient list of Dr. Howard Rashba into an electronic medical record utilizing eClinical works • Observed daily patient check-ups, vaccinations and testing procedures for multiple infectious diseases • Exposed to appointments with patients having special needs and learning disabilities

Education

  • Wheaton College (MA)
    Bachelor of Arts (B.A.), Chemistry
    2011 - 2015
  • Stoneham High School
    High School Diploma
    2007 - 2011

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