Nicholas Keith

PhD Candidate at The Johns Hopkins University School of Medicine
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Location
Baltimore, Maryland, United States, US
Languages
  • English Native or bilingual proficiency
  • French Elementary proficiency
  • Japanese Elementary proficiency

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Experience

    • United States
    • Hospitals and Health Care
    • 700 & Above Employee
    • PhD Candidate
      • Aug 2019 - Present

      • Lab of Joshua Modell, Ph.D. studying the mechanisms of bacterial CRISPR-Cas immunity. • Lab of Joshua Modell, Ph.D. studying the mechanisms of bacterial CRISPR-Cas immunity.

    • United States
    • Higher Education
    • 1 - 100 Employee
    • Research Specialist
      • Aug 2018 - Jul 2019

      • Lab of Mark Rasenick, Ph.D. in the Dept. of Physiology and Biophysics • Investigated the effects of antidepressants on cellular G-protein localization in cell culture models and human blood extracts • Experience with the ProteinSimple Wes protein quantification platform, confocal microscopy, and cell-based drug response assays • Lab of Mark Rasenick, Ph.D. in the Dept. of Physiology and Biophysics • Investigated the effects of antidepressants on cellular G-protein localization in cell culture models and human blood extracts • Experience with the ProteinSimple Wes protein quantification platform, confocal microscopy, and cell-based drug response assays

    • United States
    • Biotechnology Research
    • Operations Manager
      • Sep 2017 - May 2018

      • Biotechnology company specializing in the expression and purification of GTPases, Phospholipases, and biosensors • Managed the research and acquisition of capital expenditures • Executed product packaging and shipment to domestic and international customers • Biotechnology company specializing in the expression and purification of GTPases, Phospholipases, and biosensors • Managed the research and acquisition of capital expenditures • Executed product packaging and shipment to domestic and international customers

    • United States
    • Higher Education
    • 700 & Above Employee
    • Research Assistant
      • Feb 2015 - May 2018

      • Lab of John Sondek, Ph.D., advised by Research Assistant Professor Nicole Hajicek, Ph.D. • Investigated the dysregulation of Phospholipase C isozymes in cancer • Experience in mammalian tissue culture, PCR, plasmid mutagenesis and purification, gel electrophoresis, cellular transfection and transformation • Research successfully defended in Honors Thesis presentation “Dysregulation of Phospholipase C gamma1 in Adult T-cell Leukemia/Lymphoma” • Lab of John Sondek, Ph.D., advised by Research Assistant Professor Nicole Hajicek, Ph.D. • Investigated the dysregulation of Phospholipase C isozymes in cancer • Experience in mammalian tissue culture, PCR, plasmid mutagenesis and purification, gel electrophoresis, cellular transfection and transformation • Research successfully defended in Honors Thesis presentation “Dysregulation of Phospholipase C gamma1 in Adult T-cell Leukemia/Lymphoma”

    • China
    • Research
    • 700 & Above Employee
    • International Researcher
      • Jun 2017 - Jul 2017

      • Lab of Jianye Zang, Ph.D. through the USTC Future Biologists Summer Program • Investigated the crystallization of S. aureus virulence factor SSL13 • Presented findings at the end of the program • Lab of Jianye Zang, Ph.D. through the USTC Future Biologists Summer Program • Investigated the crystallization of S. aureus virulence factor SSL13 • Presented findings at the end of the program

Education

  • The Johns Hopkins University School of Medicine
    Doctor of Philosophy - PhD, Biochemistry, Cellular and Molecular Biology
    2019 -
  • University of North Carolina at Chapel Hill
    B.S. with Honors in Biology, B.A. Chemistry
    2014 - 2018

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