Nele de Klerk
Team leader Micropia Laboratory at Micropia- Claim this Profile
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Dutch Native or bilingual proficiency
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English Full professional proficiency
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Swedish Professional working proficiency
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French Elementary proficiency
Topline Score
Bio
Credentials
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FELASA C
Utrecht UniversityApr, 2007- Sep, 2024 -
Radiation Safety Level 5B
Delft University of TechnologyJan, 2005- Sep, 2024 -
Emergency Response Officer (Dutch: BHV)
EuroBHVOct, 2021- Sep, 2024
Experience
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Micropia
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Netherlands
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Museums, Historical Sites, and Zoos
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1 - 100 Employee
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Team leader Micropia Laboratory
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Sep 2017 - Present
ARTIS-Micropia is the only museum on earth that reveals the invisible world of micro-organisms. Micropia aims to create interest among the general public for these smallest and most successful organisms. In addition to a museum, Micropia is also a platform, a link between the scientific community and the public. It provides information on current issues, the importance of microbes and the possibilities they offer for people and nature. With a team of lab technicians, interns and volunteers the Laboratory at Micropia maintains a collection of a great variety of micro-organisms. In my role as Team leader I coordinate all operations of the lab and the daily maintenance of the museum exhibitions. Further, I provide a link between the laboratory personnel and other divisions within the ARTIS-organisation and external collaboration partners. Show less
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Karolinska Institutet
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Sweden
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Higher Education
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700 & Above Employee
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Laboratory Engineer
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Apr 2016 - Jun 2017
In my role as laboratory engineer I support all projects running in the research group by designing, performing and analysing experiments, ordering materials, and writing grant applications. Research group leader: Prof. Lars Olaf Cardell Research group focus: Involvement of the innate immune system and neuropeptides in airway inflammation (allergic rhinosinusitis, chronic rhinosinusitis and asthma). Skills: (primary) cell culture, PBMC and neutrophil isolation, flow cytometry, qPCR, immunohistochemistry, (fluorescence) microscopy, western blot, in vivo experiments, grant application writing Show less
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Stockholm University
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Sweden
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Research Services
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700 & Above Employee
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PhD-student
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Apr 2011 - Mar 2016
The subject of my PhD research was host-bacteria interactions. I primarily investigated the response of host epithelial cells and macrophages to bacterial colonisation by examining the expression of host cell factors, such as amphiregulin, EGR1 and cytokines, as well as the underlying signalling pathways. In these studies, the pathogens Helicobacter pylori and Neisseria gonorrhoeae were the most used bacterial species. In another project I studied how certain Lactobacillus species can reduce the colonisation capacity of Helicobacter pylori.Other responsibilities during my PhD studies included teaching (microbiology laboratory skills on both bachelor and master level, and giving lectures on the "Diversity and taxonomy of bacteria and archea"), supervising master project students, organising the move of the lab, inventory and storage of chemicals, and maintenance of a LSRFortesssa flow cytometer.Skills: science communication (writing of peer-reviewed papers, conference oral and poster presentations), university level teaching, student supervising, bacterial culture, cell culture, qPCR, western blot, ELISA, flow cytometry, recombinant protein expression, FPLC, in vivo experiments, fluorescence microscopy, light microscopy Show less
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Project work
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Jun 2010 - Mar 2011
VEGF and follistatin expression in host epithelial cells during Neisseria meningitidis infection.Skills: bacterial culture, cell culture, western blot, qPCR
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Intern
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Oct 2009 - Feb 2010
The effect of gonococcal infection on the host cell factor amphiregulin. Skills: Bacterial culture, cell culture, ELISA, western blot, microinjection The effect of gonococcal infection on the host cell factor amphiregulin. Skills: Bacterial culture, cell culture, ELISA, western blot, microinjection
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UMC Utrecht
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Hospitals and Health Care
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1 - 100 Employee
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Teaching assistant
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2006 - 2010
Teaching assistant for courses within the Biomedical Sciences and Medicine study programs at Utrecht University. I thought students about tissue and cell morphology, mainly through light microscopy.
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Intern
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Oct 2007 - Jul 2008
Age differences in T cellsSkills: T cell isolation, flow cytometry, qPCR, western blot
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Erasmus MC
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Netherlands
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Hospitals and Health Care
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700 & Above Employee
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Intern
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Mar 2009 - Aug 2009
Antigenicity of Madurella mycetomatis Skills: recombinant protein expression, western blot, PCR, DNA sequencing, Luminex, fungal culture, scientific writing Antigenicity of Madurella mycetomatis Skills: recombinant protein expression, western blot, PCR, DNA sequencing, Luminex, fungal culture, scientific writing
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Education
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2011 - 2016
Stockholm University
Doctor of Philosophy (PhD), Molecular Bioscience -
2007 - 2010
Universiteit Utrecht
Master of Science (M.Sc.), Biomedical Sciences, Infection and Immunity programme -
2004 - 2007
Universiteit Utrecht
Bachelor of Science (B.Sc.), Biomedical Sciences
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