Experience

Life & Soft

• France
• Information Technology & Services
• 1 - 100 Employee

• Clinical Research Project Lead, Women's Health, Fertility and Probiotics

  • Jan 2021 - Present

  Île-de-France, France Project Lead in the development of novel therapeutic approaches linked to the vaginal microbiota and women’s health. I focus mainly on fertility, inflammation, endometriosis and pregnancy outcomes. Active analyst on the omics and on the bech teams Still working at IDMIT (CEA) lab Techs: Shotgun, Targetted NGS, Transcriptomics, scRNAseq



CEA

• Chile
• Industrial Machinery Manufacturing

• Postdoctoral Researcher, vaginal microbiota, functional biology and probiotics

  • Oct 2018 - Dec 2020

  Fontenay-aux-Roses The immune response in the vaginal mucosa is modulated by different factors where the microbiota that inhabit this environment plays a major role. In healthy women, the vaginal microbiota is rich in aerobic bacteria and is dominated by lactobacillus. A healthy environment (also called eubiotic) is anti-inflammatory and protects women against pathogens. However, an alteration of the microbiota composition – or dysbiosis – is associated to a pro-inflammatory environment… Show more The immune response in the vaginal mucosa is modulated by different factors where the microbiota that inhabit this environment plays a major role. In healthy women, the vaginal microbiota is rich in aerobic bacteria and is dominated by lactobacillus. A healthy environment (also called eubiotic) is anti-inflammatory and protects women against pathogens. However, an alteration of the microbiota composition – or dysbiosis – is associated to a pro-inflammatory environment, detrimental for women health. Chlamydia trachomatis is the agent causing a sexually transmitted urogenital infection. This STI is the most prevalent infection in women that are also infected with HIV-1. The infection with CT provokes an inflammation that increases susceptibility to HIV-1. However, up to now, there is no study probiotic on the marked that lowers the risk for STI acquisition. I aim to select a probiotic strain to modify vaginal microbiota and disminish the risk for HIV-1 and Chlamydia infection using the preclinical macaque cynomolgus in vivo model and cervical cell lines as the in vitro model for signaling characterization. Show less



French National Institute for Agricultural Research

• France
• Research
• 700 & Above Employee

• PhD project, Functional microbiology and host-bacteria interactions

  • Oct 2014 - Oct 2017

  Enterococcus faecalis is a natural commensal of the human microbiota. Under certain conditions such as immunodeficiency and antibiotic treatment, E.faecalis can translocate trough the intestinal epithelium and infect the bloodstream and multiple organs producing systemic infections. My host lab identified a novel virulence facteur and named it ElrA (Enterococcal Leucin Rich). It is a surface protein internalin-like that has an important role in virulence (deleted mutant is attenuated for… Show more Enterococcus faecalis is a natural commensal of the human microbiota. Under certain conditions such as immunodeficiency and antibiotic treatment, E.faecalis can translocate trough the intestinal epithelium and infect the bloodstream and multiple organs producing systemic infections. My host lab identified a novel virulence facteur and named it ElrA (Enterococcal Leucin Rich). It is a surface protein internalin-like that has an important role in virulence (deleted mutant is attenuated for virulence in an in vivo model). It interacts with a eukaryotic protein. The main objective of my PhD is identifying the role of ElrA in the infectious process, its roles during infection, and signalization pathways implicated. Plus, correlate the data with the roles of the identified partner protein in the host Supervisors: Dr. Pascale Serror and Dr. Cristel Archambaud Techniques used: Cell culture, Cell infection, Protein purification, Western blotting, Immunofluorescence, Live-imaging, Animal experimentation Show less



Institut Pasteur

• France
• Research Services
• 700 & Above Employee

• Master Internship, Host epigenetics and Chlamydia trachomatis infection

  • Nov 2013 - Aug 2014

  Paris Bacterial epigenetic control of the host is a novel emerging subject in microbiology. Many bacteria such as Listeria monocytogenes, Legionella pneumophila and Chlamydia trachomatis have been identified as having virulence factors that specifically target the host nuclei. C. trachomatis is a intracellular pathogen that possesses a secreted protein NUE (NUclear Effector) was the first protein identified in bacteria as having a tropism for the host cell nuclei, binding to chromatin and… Show more Bacterial epigenetic control of the host is a novel emerging subject in microbiology. Many bacteria such as Listeria monocytogenes, Legionella pneumophila and Chlamydia trachomatis have been identified as having virulence factors that specifically target the host nuclei. C. trachomatis is a intracellular pathogen that possesses a secreted protein NUE (NUclear Effector) was the first protein identified in bacteria as having a tropism for the host cell nuclei, binding to chromatin and inducing a methylation in histones that control the expression of human genes. During the second year of my master degree I identified many potential Chlamydial proteins as putative nuclear effectors that have a tropism for the host's nuclei and that are secreted by a Type III Secretion System. Supervisor: Dr. Agathe Subtil Techniques used: Cellular transfection, Immunofluorescence and Microscopy, Subcellular fractionation, Electrophoresis and Western Blotting, Primer design, Cloning and transformations, Sequencing, Protein extraction, Bioinformatics, etc. Show less



Institute of Pharmacology and Structural Biology (CNRS)

• Master Internship, functional microbiology, biochemistry

  • Nov 2012 - Dec 2012

  Toulouse, France Mycobacterium abcessus is an opportunistic pathogen responsible of infections in patients with cystic fibrosis. A mutant of a locus that encodes for a lipopeptide producer enzyme was identified as being avirulent. In this project, an inverse genetic approach was used in order to identify a lipopeptide implicated in virulence by comparing phenotypes with the wild type bacteria. Supervisor: Dr. Gilles Etienne

• Bachelor Internship

  • Jul 2012 - Aug 2012

  Toulouse, France Caracterisation of two avirulent mutants implicated in the production of lipoproteins in Mycobacterium abscesses Techniques used: Bacterial culture in P2+, total lipid extraction, fractionation by column elution, thin layer chromatography, mass spectrometry and magnetic resonance, bioinformatics



Universidad Peruana Cayetano Heredia

• Peru
• Higher Education
• 700 & Above Employee

• Bachelor Internship

  • Jul 2008 - Dec 2008

  Laboratory of ecotoxicology and bioremediation Asseting the capacity of Aloe sp. for capturing contaminating copper in polluted water.

• Bachelor Internship

  • Jan 2008 - Mar 2008

  Laboratory of Biology and Physiological sciences