Experience

Kodiak Sciences

• United States
• Biotechnology Research
• 1 - 100 Employee

• Scientist II

  • Jul 2022 - Present

• Scientist I

  • Aug 2021 - Jun 2022

• Associate Scientist

  • Jan 2019 - Jul 2021

  San Francisco Bay Area

• Senior Research Associate

  • Oct 2016 - Dec 2018

  San Francisco Bay Area



Teknova

• United States
• Biotechnology Research
• 100 - 200 Employee

• Research Associate - Protein Engineering and Molecular Biology

  • Jan 2016 - Oct 2016

  San Francisco Bay Area .Protein design, selection and high throughput screening to identify and characterize novel scFv proteins .Design of gene constructs and primers, perform molecular cloning, vector design and phage display and panning .Routinely perform PCR, gel electrophoresis, ELISA, plasmid DNA isolation using minipreps, and nucleic acid quantitation



University of California, San Francisco

• United States
• Higher Education
• 700 & Above Employee

• Associate Specialist

  • Nov 2013 - May 2015

  San Francisco Bay Area .Sample preparation for mass spectrometry analysis, antibody assays, western blot and SDS-PAGE .HPLC separation of proteins and peptides, protein quantitation. .HPLC, Progest, and plate reader operations, maintenance and troubleshooting .Data Interpretation using ProteinPilot, MASCOT and other online proteomics tools .Documentation of experiments, results, findings, technical reports, SOP's and analytical method protocols. .Maintenance of core website built on Drupal7 using HTML… Show more .Sample preparation for mass spectrometry analysis, antibody assays, western blot and SDS-PAGE .HPLC separation of proteins and peptides, protein quantitation. .HPLC, Progest, and plate reader operations, maintenance and troubleshooting .Data Interpretation using ProteinPilot, MASCOT and other online proteomics tools .Documentation of experiments, results, findings, technical reports, SOP's and analytical method protocols. .Maintenance of core website built on Drupal7 using HTML and CSS. Show less



Illumina

• United States
• Biotechnology Research
• 700 & Above Employee

• Curation Scientist

  • Jan 2013 - Nov 2013

  San Francisco Bay Area .Population and curation of databases with the results of the analysis of gene expression (Microarray-, sequence-based) data .Updating the company’s ontology application with new phenotypes .Quality analysis of clinical data - XML files and annotation files.



CureMeta LLC

• United States
• Biotechnology Research
• 1 - 100 Employee

• Co-op Scientist

  • Jul 2012 - Dec 2012

  Greater Boston Area -Worked primarily on Metastatic cancer research: Prostate and Breast cancer -Maintenance and expansion of embryonal cancer stem cell lines under normal and hypoxic conditions -Transfection of cancer stem cell lines .Used Fugene, Lonza Nucleofector system and Invitrogen Neon Transfection system .Compared trasfection efficiency using the various methods -Performed various cell-based assays in 12-,48-,and 96- well plates. .Studied cell invasion/metastasis using cell… Show more -Worked primarily on Metastatic cancer research: Prostate and Breast cancer -Maintenance and expansion of embryonal cancer stem cell lines under normal and hypoxic conditions -Transfection of cancer stem cell lines .Used Fugene, Lonza Nucleofector system and Invitrogen Neon Transfection system .Compared trasfection efficiency using the various methods -Performed various cell-based assays in 12-,48-,and 96- well plates. .Studied cell invasion/metastasis using cell migration assays (Oris Cell Migration assay and Invitrogen Cultrex Cell Migration assay, 3D cell spheroid assays) .Cell viability (MTT assay) to study the effect of acetonitrile as a peptide solvent on cancer stem cell viability -Analyzed protein levels using several protein assays( BCA , Bradford) -Tested for biomarker using ELISA, Western Blot and Dot blot -Performing protein purification using PNA beads -Tested and compared three different versions of fetal Bovine Serum and helped in determine which was the best in terms of growth rate and cell health as well as cost. -Maintained a regularly updated inventory of all the different media and media components in the lab that helped in the ordering process Show less



Northeastern University

• United States
• Higher Education
• 700 & Above Employee

• Graduate Research Assistant

  • Jan 2011 - Jun 2012

  Greater Boston Area .Investigated the neuro-protective effects of nicotine on dopiminergic cells under the guidance of Dr. R.H.Loring .Cell toxicity and viability assays (Propidium Iodide, MTT, Calcein AM, Trypan Blue) using 6- hydroxydopamine as a neurotoxin. · Characterized receptor subunits found in SKNAS and SKNSH cell lines. · Work involves cell culture, SDS-PAGE, PCR, fluorescent microscopy and analysis of the results

• Assistant for International Student Services

  • Sep 2011 - May 2012

  Greater Boston Area .Help International Students with Immigration clearance, Co-op related paperwork, OPT filing, Data entry.



Biocon

• India
• Biotechnology Research
• 700 & Above Employee

• Process Development and Pharmaceuticals Intern

  • Sep 2009 - Feb 2010

  Bangalore, India · Worked at the Process Development and Pharmaceuticals R&D lab. · Optimized the media and process conditions for Mycophenolic Acid (MPA) production using DOE · Tested raw materials for media preparation using protein assays and Ion Exchange Chromatography (Dionex). .Studies the effect of nutrients on MPA production .Shake flask and pilot scale fermentation of Penicillium fungi for MPA production .Analysis of the sample yield using Agilent 1100/1200 Series Reverse Phase -… Show more · Worked at the Process Development and Pharmaceuticals R&D lab. · Optimized the media and process conditions for Mycophenolic Acid (MPA) production using DOE · Tested raw materials for media preparation using protein assays and Ion Exchange Chromatography (Dionex). .Studies the effect of nutrients on MPA production .Shake flask and pilot scale fermentation of Penicillium fungi for MPA production .Analysis of the sample yield using Agilent 1100/1200 Series Reverse Phase - HPLC syste and Gas Chromatography (Agilent and Waters), GE ÄKTA - Explorer 100 and Purifier 10 HPLC system, AKTA Prime plus system .Cell culture .Data analysis Show less



Macmillan

• United States
• Book and Periodical Publishing
• 700 & Above Employee

• Copy Editor

  • Mar 2009 - Sep 2009

  .Critical editing of Scientific Books and Online journals. .Client included Elsevier, Mendeley and Wiley



Infosys

• India
• IT Services and IT Consulting
• 700 & Above Employee

• Software Engineer

  • Sep 2008 - 2009

  Software design and development in .NET, C and C++.