Postdoctoral Scholar

• Dec 2021 - Present

-High dimensional single nuclei characterization of brain immune compartments including infiltrating and resident immune cells residing in the parenchyma and in nonparenchymal structures, such as perivascular spaces, subdural meninges and the choroid plexus using sn-RNAseq and/ ATAC-seq, or snMultiome ATAC + Gene Expression. -Sample multiplexing with barcoding substantially increasing the number of samples -3D organoid cultures -High dimensional single nuclei characterization of brain immune compartments including infiltrating and resident immune cells residing in the parenchyma and in nonparenchymal structures, such as perivascular spaces, subdural meninges and the choroid plexus using sn-RNAseq and/ ATAC-seq, or snMultiome ATAC + Gene Expression. -Sample multiplexing with barcoding substantially increasing the number of samples -3D organoid cultures

Visiting Scientist

• Dec 2021 - Feb 2022

Postdoctoral Scientist

• Jul 2020 - Nov 2021

Project: Endogenous retroviruses augment amphibian (Xenopus laevis) tadpole antiviral protection. Techniques involved: Animal handling and dissections for scientific procedures, organ/tissue isolations, cell cultures maintenance and treatments, Crispr-cas9, Guide RNA design, microinjections and other Crispr-cas9 related tools, Macvector files, plasmid based gene delivery, cloning, transformation and transfection, chemotaxis, protein isolation and purification, western blot, RNA and DNA isolations, RT-PCRs, qRT-PCRs, Viral load analysis, In-silico tools, bone-marrow culture, kidney culture, Histology- cyto-spinning, specific and non-specific tissue staining. Statistical analysis. Show less

Whitman Visiting Scientist

• Aug 2021 - Aug 2021

National Xenopus Resource (NXR) Crispr-cas9, Guide RNA design, microinjections and other Crispr-cas9 related tools and Macvector files National Xenopus Resource (NXR) Crispr-cas9, Guide RNA design, microinjections and other Crispr-cas9 related tools and Macvector files

Senior Research Fellow

• Dec 2013 - Jul 2019

Techniques involved: Bioinformatics- NCBI, dbSNP database, Ensemble genome browser, Evolutionary conserved regions identification, Primer-Blast, Primer3, NEB cutter, Protein Data Bank, SNPinfo (FuncPred) and RegulomeDB, PolymiRTS (v 3.0), SIFT and Polyphen-2 servers, protein structural analysis using I-TASSER, RAMPAGE, Swiss-PdbViewer, Chimera and I- mutant, molecular dynamics and energy minimization calculations etc. Molecular- PCR-RFLP, ARMS-PCR, Reverse transcriptase-PCR, DNA RNA extraction, DNA/RNA purification and quantification, Sequencing, Molecular cloning, competent cell preparation, ligation, Transformation. Animal cell culture- Maintenance of cell culture, Cryopreservation of cell cultures, Cell counting, Cytotoxicity assay, Mechanistic studies such as Immunocytochemistry. Microbial- Microbial cell culture, cell counting, wet mount, Gram Staining, species identification, Antimicrobial assay, Micorbial adhesion assay on animal cell lines. Biochemistry- Spectrophotometeric analysis, enzymatic assays, TAC, TOS, OSI, protein estimation using modified manual standard protocols. Immunology-ELISA, Lymphocytes isolation, double immunodiffusion, rocket electrophoresis, Immunoelectrophoresis, Agglutination reactions Statistical software packages- Capable of handling range of software packages to analyse data - from MS Excel to dedicated packages, such as SPSS, GraphPad, MedCalc, ProMeta Ver. 3 and SNPStats for statistical analysis of quantitative data. Statistical techniques-Simple descriptive statistics to advanced parametric and Non-parametric test, such as (a) Independent samples t-test (parametric) and its corresponding non-parametric test i.e. Mann-U-Whitney test. (b) One-way ANOVA (parametric) and its corresponding non- parametric test i.e. Kruskal-Wallis test. (c) Pearson correlation (d) multiple linear regressions (e) Chi-square (f) Fisher’s exact test (g) odds ratio statistics (h) Multifactor Dimensionality Reduction (i) Meta-analysis Show less