Experience

Panacea Biotec Ltd

• Scientific Officer



Enzene Biosciences

• BTFS Intern

  • Apr 2015 - Aug 2015

  WORKED ON A PROJECT ENTITLED,"SCALING UP OF RECOMBINANT PROTEIN FOLLOWED BY ITS PURIFICATION USING Ni-Sepharose CHROMATOGRAPHIC TECHNIQUE AND ENZYMATIC STUDY ASSAYS.UPSTREAM PROCESS:• Preparation of Growth media (POWER-CHO) and its sterilization by dead-end filtration method(0.22 micron)• Preparation of antifoam reagent and feed supplement (CB3 &CB4)• Seed Preparation in shake flask culture and scale up in bioreactor (Sartorius- Stedim) followed by continuous monitoring of Ph… WORKED ON A PROJECT ENTITLED,"SCALING UP OF RECOMBINANT PROTEIN FOLLOWED BY ITS PURIFICATION USING Ni-Sepharose CHROMATOGRAPHIC TECHNIQUE AND ENZYMATIC STUDY ASSAYS.UPSTREAM PROCESS:• Preparation of Growth media (POWER-CHO) and its sterilization by dead-end filtration method(0.22 micron)• Preparation of antifoam reagent and feed supplement (CB3 &CB4)• Seed Preparation in shake flask culture and scale up in bioreactor (Sartorius- Stedim) followed by continuous monitoring of Ph, DO. • Offline monitoring of Glucose, Glutamine and its maintenance using Cedex Bioanalyzer (ROCHE)• Physical monitoring of osmolarity using osmometer 3500 (Advanced Intruments)Downstream processing:• Calibration of analytical instruments (Ph meter, conductivity meter & turbidometer).• Buffer preparation (Dialysis buffer, drug substance buffer,solubilization buffer & lysis buffer) and its filtration by 0.45 micron membrane filtration• Cells thawing and lysis using homogenization process by Microtecnik homogenizer.• Preparation of cell lysate by ultra-homozenization technique ( GEA 2000 PANDA PLUS).• Purification of recombinant protein-Liraglutide by affinity chromatography technique using Ni-Sepharose column (GE AKTA EXPLORER 100, GE AKTA PURIFIER 10).• Concentration of recombinant protein using dialysis method & TFF system.• Protein estimation using Bradford method (Bio-rad micro plate reader) & analysis of protein band using SDS-PAGE method• Packing of column , regeneration & CIP Show more Show less



Quintiles

• Safety Associate

  • Apr 2015 - Apr 2015



Probiosys

• BTFS INTERN IN FERMENTATION AND BIOPROCESS ENGINEERING

  • Nov 2014 - Mar 2015

  Worked on a project titled as "Isolation and Purification of protease enzyme from Bacillus sp.UPSTREAM PROCESS-Isolation of Bacillus sp from sewage water-Microbial techniques for characterisation of the sp..- Performance of different Biochemical test ( IMVIC, STARCH TEST, LYSINE DECARBOXYLASE TEST, SIMMONS CITRATE TEST).-Large scale growth of isolated (Bacillus sp.) organism using shaker flask culture.DOWNSTREAM PROCESS-Centrifugation of the isolated… Worked on a project titled as "Isolation and Purification of protease enzyme from Bacillus sp.UPSTREAM PROCESS-Isolation of Bacillus sp from sewage water-Microbial techniques for characterisation of the sp..- Performance of different Biochemical test ( IMVIC, STARCH TEST, LYSINE DECARBOXYLASE TEST, SIMMONS CITRATE TEST).-Large scale growth of isolated (Bacillus sp.) organism using shaker flask culture.DOWNSTREAM PROCESS-Centrifugation of the isolated organism-Purification of the enzyme using Dialysis method-Ion exchange chromatography-Molecular techniques- SDS-PAGE 2D, AGAROSE GEL ELECTROPHORESIS. Isolation of catalase from chicken liver. Isolation of Lysozyme enzyme from egg white.Isolation of Amylase from Aspergillus niger using solid state fermentation method in static culture flask(1000ml).Fermentation Technique- Production of Alcoholic beverages and its distillation process.Clean Room Validation Show more Show less



Lablinks Biotech Pvt. Ltd

• Trainee

  • May 2014 - Jul 2014

• BCIL TRAINEE

  • Nov 2013 - Apr 2014