Experience

University of North Carolina at Chapel Hill

• United States
• Higher Education
• 700 & Above Employee

• Postdoctoral Researcher

  • Jun 2019 - Present

  Characterized the shared role of BMP regulators, SMAD6 and SMAD7 in vascular flow response using both mouse embryos and human umbilical vein endothelial cells (HUVEC) - Implement human iPSC differentiation to endothelial cells using a cytokine-based method - Optimize confocal microscopy methods to analyse the effect of vascular specific deletion of SMAD6 and SMAD7 in mid-gestation mouse embryos - Perform laminar flow experiments using the Ibidi system to understand the effects of BMP signalling manipulations on endothelial cell flow response - Perform RNA sequencing on HUVEC after siRNA knockdown of SMAD6 and SMAD7 in static and flow conditions Show less



EMBL

• Germany
• Research Services
• 700 & Above Employee

• PhD Student

  • May 2015 - May 2019

  Leveraged single cell transcriptomics and multi-color flow cytometry to identify CD44 as a cell surface marker of the earliest stage of hematopoietic stem cell differentiation in mouse embryos - Routinely used MACS and FACS to enrich, isolate and analyse endothelial and hematopoietic cells derived from mouse dorsal aorta and mouse embryonic stem cells - Manipulated levels of CD44 expression in a mouse embryonic stem cell differentiation system to understand the effect of CD44 on the endothelial to hematopoietic transition. - Profiled single cells with varying levels of CD44 expression using quantitative PCR analysis and analysed results using R coding language - Implemented CRISPR-Cas9 genome editing in the lab to generate knockout and over-expression mouse embryonic stem cell lines Show less



Monash University

• Australia
• Higher Education
• 700 & Above Employee

• Internship with the Department of Anatomy and Developmental Biology

  • Jun 2012 - Jan 2014

  Awarded scholarship to participate in the Bio21 Cluster's undergraduate research opportunities program. Awarded scholarship to participate in the Bio21 Cluster's undergraduate research opportunities program.