Morgan Gargulak

Biology and Chemistry Teacher at Edmund Burke School
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Contact Information
us****@****om
(386) 825-5501
Location
Washington, District of Columbia, United States, US

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Experience

    • United States
    • Education Management
    • 1 - 100 Employee
    • Biology and Chemistry Teacher
      • Jun 2019 - Present
    • Bulgaria
    • Museums, Historical Sites, and Zoos
    • 1 - 100 Employee
    • Public Engagement Volunteer, Q?rius and Q?rius Jr: A Discovery Room
      • Jun 2017 - Present

      Engage visitors, ages 17 and under, in asking questions and learning about science, nature, and culture. Guide visitors through hands-on science exhibits to promote interaction with over 6,000 museum specimens, microscopes, and special programming. Facilitate visitor interaction with staff scientists to enhance understanding of scientific research.

    • Public Engagement Volunteer, Outbreak: Epidemics in a Connected World
      • Apr 2018 - Jun 2019

      Facilitate inquiry-based science activities with multi-generational visitors to promote engagement with exhibits. Promote learning about how human health, animal health, and environmental health are intrinsically linked by encouraging visitors to ask questions and attentively answering those questions.

    • India
    • Hospitals and Health Care
    • 100 - 200 Employee
    • Biologist
      • Jan 2017 - Dec 2018
    • Biologist
      • Mar 2016 - Jan 2017

      Contractor to the NIH- National Cancer Institute. Contractor to the NIH- National Cancer Institute.

    • United States
    • IT Services and IT Consulting
    • 1 - 100 Employee
    • Laboratory Technician IV
      • May 2014 - Mar 2016

      Contractor to the Walter Reed Army Institute of Research. Process blood to isolate human PBMC and plasma in support of regulated activities. Perform immunological assays to explore the effectiveness of Dengue vaccines used in ongoing clinical trials. Technical skills: Isolation of peripheral blood mononuclear cells (PBMC) from human blood samples using either Cell Preparation Tubes (CPTs) or ficoll, flow cytometry using BD LSRFortessa, FlowJo vX for PC, intracellular cytokine staining (ICS) of human PBMC, ex vivo surface staining of human PBMC, ELISpot, Fluorospot, Bio-Plex Human Cytokine 27-Plex for Luminex MAGPIX Show less

    • United States
    • Higher Education
    • 700 & Above Employee
    • Graduate Student Researcher
      • Sep 2013 - Apr 2014

      Project 1: To address whether 53BP1 is recruited to double stranded DNA breaks (DSBs) in the absence of γ-H2AX. In Ligase IV/ H2AX-deficient cell lines, DSBs in cells in phase G1 are resected. Overexpression of RNF168 is physiologically relevant in certain types of cancer. Our goal is to determine if RNF168 is capable of recruiting 53BP1, a protein normally recruited in a γ-H2AX dependent manner, to these DSB sites, and to determine if 53BP1 is sufficient to stop resection of these sites and induce repair via the Non-Homologous End Joining pathway. Project 2: To study the role of a mutation in Atg16L1, a member of the autophagy protein family, on autophagy in colon and small intestine epithelial cells. This mutation is associated with risk of Crohn’s Disease. Autophagy defects can lead to defects in goblet cell secretion of mucus, which in turn may lead to symptoms associated with the disease. Technical skills: Flow cytometry using FACSCalibur, intracellular enzyme staining in murine cell lines, Western Blot, cell culture, retroviral infection of murine cell lines, transfection, cell sorting using MACS MicroBeads, genomic DNA isolation, restriction digest, Southern Blot Show less

    • United States
    • Chemical Manufacturing
    • 1 - 100 Employee
    • Undergraduate Research Assistant in the lab of Dr. Nikolau
      • Jun 2011 - May 2013

      The goal of this project was to understand and exploit the ability of ketoacyl-ACP synthase III (KASIII) to use different types of primers to generate diverse fatty acids, which could be used as precursors for biorenewable chemicals. In fatty acid biosynthesis, KASIII catalyzes the initial condensation between acetyl-CoA and malonyl-ACP to form acetoacetyl-ACP. Technical skills: Cloning and transformation of E. coli using TOPO pENTR cloning kits and Gateway pDEST vectors, PCR, plasmid isolation, agarose gel electrophoresis, gel purification of PCR products, protein extraction and purification using Ni-NTA chromatography, Bradford assay, SDS PAGE Show less

    • United States
    • Biotechnology Research
    • 300 - 400 Employee
    • Research Intern
      • May 2012 - Aug 2012

      Method comparison testing of immunological assays for FDA approval of diagnostic ELISA-based products. Technical skills: ELISA, Luminex Method comparison testing of immunological assays for FDA approval of diagnostic ELISA-based products. Technical skills: ELISA, Luminex

    • United States
    • Higher Education
    • 700 & Above Employee
    • Undergraduate Research Assistant in the lab of Dr. Janzen
      • Jan 2011 - May 2012

      Our goal in this study was to examine genetic variation in Blanding’s turtles to generate information that will be useful in future conservation efforts involving this species in the Midwest. Technical skills: PCR, agarose gel electrophoresis, DNA isolation Our goal in this study was to examine genetic variation in Blanding’s turtles to generate information that will be useful in future conservation efforts involving this species in the Midwest. Technical skills: PCR, agarose gel electrophoresis, DNA isolation

    • United States
    • Higher Education
    • 700 & Above Employee
    • Research Intern in the lab of Dr. Wimpee
      • Jun 2010 - Jul 2010

      McNair Post-Baccalaureate Achievement ProgramCharacterization of bioluminescent bacteria within the genus Vibrio. Assessment of ecological diversity in bioluminescent bacteria via genetic analysis.Technical skills: PCR, DNA isolation, agarose gel electrophoresis

    • Tutor
      • Sep 2009 - May 2010

      Tutored peers in Algebra, Business Calculus, Calculus 1 and 2, Introductory Biology, General Chemistry 1 and 2

Education

  • Iowa State University
    Bachelor’s Degree, Biology, General
    2010 - 2013
  • Washington University in St. Louis
    No degree, Immunology
    2013 - 2014
  • The George Washington University
    Master of Education - MEd, Secondary Education
    2017 - 2019

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