Experience

Mediar Therapeutics

• United States
• Biotechnology Research
• 1 - 100 Employee

• Senior Scientist

  • Mar 2023 - Present



Manus Bio

• United States
• Biotechnology Research
• 1 - 100 Employee

• Research Scientist, Enzyme Engineering

  • May 2020 - Dec 2022

  -Project lead responsible for collaborating with multidisciplinary teams for effective experimental planning, communication of timelines, and coordination strain scale up at an offsite facility -Led efforts to engineer various classes of proteins to improve production of several small molecules using a variety of techniques using medium throughput microbial fermentation -Developed analytical methods for GC-FID/MS to quantify production of engineered enzymes -Project lead responsible for collaborating with multidisciplinary teams for effective experimental planning, communication of timelines, and coordination strain scale up at an offsite facility -Led efforts to engineer various classes of proteins to improve production of several small molecules using a variety of techniques using medium throughput microbial fermentation -Developed analytical methods for GC-FID/MS to quantify production of engineered enzymes



The University of British Columbia

• Canada
• Higher Education
• 700 & Above Employee

• Postdoctoral Researcher

  • Apr 2017 - Mar 2020

  -Identified the catabolic pathway of several different lignin derived monoaromatic compounds in Rhodococcus jostii and Rhodococcus EP4 using RNA-sequencing and targeted gene deletions -Characterized novel cytochrome P450 and alkylphenol hydroxylase involved in alkylguaiacol O-demethylation and alkylphenol hydroxylation using FPLC, HPLC, kinetic measurements, and substrate binding -Synthesized 13C labeled dehydrogenation polymer (DHP) lignin for use in stable isotope probing experiments to identify novel bacterial enzymes in lignin depolymerization -Identified and characterized several novel dye decolorizing peroxidase enzymes from soil and sediment samples using enzyme kinetics, thermal stability and UV-Vis spectroscopy Show less



University of Utah - Employment

• United States
• Higher Education
• 700 & Above Employee

• Graduate Student

  • Aug 2010 - 2016

  -Determined the spacing requirement of cysteine residues involved in Ccs1 that are involved in the copper delivery and disulfide oxidation of Cu/Zn Superoxide dismutase 1(Sod1) by cell viability assays, western blot, bioconjugation, and purified enzyme activity -Quantified metal content and metal occupation sites of purified WT and mutant Sod1 by ICP-OES and X-ray absorption spectroscopy -Characterized the role of copper dependent peroxide turnover in the disulfide oxidation of Sod1 Show less



University of Utah - Employment

• United States
• Higher Education
• 700 & Above Employee

• Undergraduate Research Assistant

  • 2008 - 2010