Experience

Sonata Therapeutics
• United States
• Biotechnology Research
• 1 - 100 Employee
• Scientist II

  • Jul 2022 - Present


UT MD ANDERSON CANCER CENTER

• United States
• Hospitals and Health Care
• 300 - 400 Employee

• Postdoctoral Research Fellow

  • Apr 2019 - Oct 2021

  • Managed projects and collaborated with researchers, conducting live or virtual meetings and data presentations; guide the project by adapting to the changes demanded by the results. • Optimized protocols for tumor/ lung tissue dissociation, cryopreservation, and cell sorting to process for single-cell RNA sequencing enabling successful preservation of valuable human clinical trial/ mouse experiment samples that led to massive cost reduction for sequencing. • Designed and lead in… Show more • Managed projects and collaborated with researchers, conducting live or virtual meetings and data presentations; guide the project by adapting to the changes demanded by the results. • Optimized protocols for tumor/ lung tissue dissociation, cryopreservation, and cell sorting to process for single-cell RNA sequencing enabling successful preservation of valuable human clinical trial/ mouse experiment samples that led to massive cost reduction for sequencing. • Designed and lead in vivo mice study to track tumor growth reduction, lower lung metastases frequency, and prolonged survival which led to successful identification of antibody-based therapies (immune checkpoint inhibitors) that worked best in treating Non-small cell lung cancer mouse model. • Developed a 34 color antibody panel for flow cytometry analysis (Cytek Aurora) that was used to phenotype major immune components infiltrating mouse tumor-associated with response and resistance to immunotherapy-based treatments. • Designed and performed experiments utilizing flow cytometry, immunohistochemistry, RTPCR, in vivo immune cell depletion study, bulk- and single cell- RNA sequencing that led to the exploration of mechanistic aspects of response and resistance to immunotherapy based-treatments in lung cancer mouse model. • Extensive reading and writing of the latest scientific literature that resulted in manuscripts for publication and grant proposals of future research. • Efficiently managed a young lab by establishing animal research protocols, upholding health and safety protocols, maintaining equipment/software, training assistants, studying marketplace/ building connections with vendors leading to proper utilization of lab startup fund and smooth lab operations. • Systematically organized phase II clinical trial fresh tissue retrieval for critical processing and preservation and maintained clinical trial DNA/RNA sample inventory. Show less • Managed projects and collaborated with researchers, conducting live or virtual meetings and data presentations; guide the project by adapting to the changes demanded by the results. • Optimized protocols for tumor/ lung tissue dissociation, cryopreservation, and cell sorting to process for single-cell RNA sequencing enabling successful preservation of valuable human clinical trial/ mouse experiment samples that led to massive cost reduction for sequencing. • Designed and lead in… Show more • Managed projects and collaborated with researchers, conducting live or virtual meetings and data presentations; guide the project by adapting to the changes demanded by the results. • Optimized protocols for tumor/ lung tissue dissociation, cryopreservation, and cell sorting to process for single-cell RNA sequencing enabling successful preservation of valuable human clinical trial/ mouse experiment samples that led to massive cost reduction for sequencing. • Designed and lead in vivo mice study to track tumor growth reduction, lower lung metastases frequency, and prolonged survival which led to successful identification of antibody-based therapies (immune checkpoint inhibitors) that worked best in treating Non-small cell lung cancer mouse model. • Developed a 34 color antibody panel for flow cytometry analysis (Cytek Aurora) that was used to phenotype major immune components infiltrating mouse tumor-associated with response and resistance to immunotherapy-based treatments. • Designed and performed experiments utilizing flow cytometry, immunohistochemistry, RTPCR, in vivo immune cell depletion study, bulk- and single cell- RNA sequencing that led to the exploration of mechanistic aspects of response and resistance to immunotherapy based-treatments in lung cancer mouse model. • Extensive reading and writing of the latest scientific literature that resulted in manuscripts for publication and grant proposals of future research. • Efficiently managed a young lab by establishing animal research protocols, upholding health and safety protocols, maintaining equipment/software, training assistants, studying marketplace/ building connections with vendors leading to proper utilization of lab startup fund and smooth lab operations. • Systematically organized phase II clinical trial fresh tissue retrieval for critical processing and preservation and maintained clinical trial DNA/RNA sample inventory. Show less



The University of Texas at Arlington

• United States
• Higher Education
• 700 & Above Employee

• Research Fellow

  • Aug 2018 - Mar 2019

• Graduate Student (Research experience)

  • Sep 2013 - Aug 2018

  • Engineered amino acid substitution mutations in the protein coding DNA sequence. Transformed the newly created mutant plasmid in Escherichia coli cells and expressed protein. Purified the protein tagged with six-Histidine residues in the C-terminal region using Talon (Co++) resin by affinity based column chromatography. • Subjected the proteins to mechanistic studies involving enzymatic assays to test protein encoded endonuclease activity, reverse transcriptase activity and target DNA/RNA… Show more • Engineered amino acid substitution mutations in the protein coding DNA sequence. Transformed the newly created mutant plasmid in Escherichia coli cells and expressed protein. Purified the protein tagged with six-Histidine residues in the C-terminal region using Talon (Co++) resin by affinity based column chromatography. • Subjected the proteins to mechanistic studies involving enzymatic assays to test protein encoded endonuclease activity, reverse transcriptase activity and target DNA/RNA binding ability. Visualized results using polyacrylamide Electrophoretic mobility shift assay (EMSA) and urea Denaturing gel. • Constructed and analyzed a homology based 3D model of the protein using bioinformatics tool. • Designed primers and oligo, used PCR and annealing method to construct double stranded and holiday junction DNA. Radiolabeled the DNA constructs with Phosphorus-32 (32P) isotope. • Optimized IPTG induced recombinant protein expression protocol and affinity chromatography based protein purification protocol to enhance the protein yield. • Gained expertise in managing multiple research projects involving research plan development, literature review, design & execution of experiments, optimization of protocols, data analysis; and participate in writing grant proposals and manuscripts. • Experience in laboratory inventory management and budgeting by taking responsibility for lab reagents ordering; material storage; and proper disposal of dangerous chemicals including radioactive materials • Served as a mentor to four graduate students and two undergraduate students providing training for research work • Maintenance and troubleshooting of lab equipment and training new users from different departments • Experience of wet laboratory systems including radiation handling station, and fume hood • 1 article in peer-reviewed journal and co-author of five oral and poster presentations

• Graduate Teaching Assistant

  • Aug 2013 - May 2018

  • Instructed undergraduate Cell and Molecular Biology labs, evaluated lab reports, quizzes and final exams • Demonstrated experiments and explained principles behind them • Supervised all laboratory activities



Rajbhandari Pharmaceuticals, Om Hospital and Research Center

• Pharmacist-In-charge

  • Feb 2012 - Jun 2013

  • One to one interaction with patients to go over all prescribed medications, educate patients on the role of each medication in their regimen, and emphasize the importance of adherence for a better outcome. • Supervision of daily management of pharmacy and delegate staff responsibilities. • Establishment and supervision of record keeping system for purchase, sale, delivery, and storage of pharmaceuticals. • Monitored and maintained inventory. • Supervision of all aspects of the… Show more • One to one interaction with patients to go over all prescribed medications, educate patients on the role of each medication in their regimen, and emphasize the importance of adherence for a better outcome. • Supervision of daily management of pharmacy and delegate staff responsibilities. • Establishment and supervision of record keeping system for purchase, sale, delivery, and storage of pharmaceuticals. • Monitored and maintained inventory. • Supervision of all aspects of the operation. Show less • One to one interaction with patients to go over all prescribed medications, educate patients on the role of each medication in their regimen, and emphasize the importance of adherence for a better outcome. • Supervision of daily management of pharmacy and delegate staff responsibilities. • Establishment and supervision of record keeping system for purchase, sale, delivery, and storage of pharmaceuticals. • Monitored and maintained inventory. • Supervision of all aspects of the… Show more • One to one interaction with patients to go over all prescribed medications, educate patients on the role of each medication in their regimen, and emphasize the importance of adherence for a better outcome. • Supervision of daily management of pharmacy and delegate staff responsibilities. • Establishment and supervision of record keeping system for purchase, sale, delivery, and storage of pharmaceuticals. • Monitored and maintained inventory. • Supervision of all aspects of the operation. Show less