Mitchell Harling
PHD Candidate at Brown University School of Engineering- Claim this Profile
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Bio
Credentials
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Basic Mamallian Cell Culture (Biosafety Level 2)
University of MaineFeb, 2017- Nov, 2024 -
Class I-IV Laser Safety Training
University of MaineFeb, 2017- Nov, 2024
Experience
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PHD Candidate
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Brown University School of Engineering
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United States
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Higher Education
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1 - 100 Employee
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PHD Student
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Sep 2020 - Present
Biomedical Engineering Ph.D. program.
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Teaching Assistant
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Sep 2021 - Dec 2021
Supervised laboratory sections and held weekly office hours for ENGN 0510 - Electricity and Magnetism.
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Brown University Pre-College Programs
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United States
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Higher Education
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1 - 100 Employee
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Course Instructor
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Jul 2021 - Jul 2022
Taught Introduction to Engineering: a 2-week course for pre-college students in the summer of 2021 and 2022. Taught Introduction to Engineering: a 2-week course for pre-college students in the summer of 2021 and 2022.
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University of Maine
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United States
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Higher Education
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700 & Above Employee
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Research Assistant
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Dec 2018 - Sep 2020
Working in the CompuMAINE Laboratory.· Focused on colocalization and image characterization techniques through ImageJ and Python Scripting.· Adapted astrophysical image analyses to a biomedical regime in ImageJ to explore collagen and nerve fiber colocalization for a study of neuropathy.· Member of a machine learning subgroup that studied machine learning theory using Python and MATLAB. Gained basic machine learning knowledge from lectures and assigned readings; the goal is to implement a neural network in the lab to improve image analyses. · Used an inhouse Tcl/Tk-based software to take anisotropy measurements of nerve and collagen fibers. This required trouble-shooting and altering source codes to get the proper measurements.
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Research Assistant
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Mar 2017 - Sep 2020
Working in the Tilbury Photonics Laboratory.· Shared the responsibility of the design, construction, and alignment of a home-built two-photon system.· Performed intensive imaging of nerve and collagen in mouse adipose tissue, using two-photon fluorescence and second harmonic generation microscopy, respectively.· Explored fibroblast growth and proliferation on a potential bone substitute, cellulose nanofiber, through two-photon microscopy and scripting 3D cell counting in FIJI.· Performed optical metabolic imaging of macrophages to study their metabolic activity in different disease states.· Maintenance of BSL-2 cell culture lines and aided in the establishment of culture protocol.· Named as a University of Maine Center for Undergraduate Research Scholar.
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Teaching Assistant
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Jan 2019 - May 2019
Assisted 1st year biomedical engineering students in Introduction to Bioengineering II with classwork related to Mathcad, R, and LabVIEW.Graded classwork and homework.
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Peer Educator
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Jan 2017 - May 2018
Assisted in recitations for general chemistry for three semesters.
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Private Tutor
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Feb 2017 - May 2017
· Tutored a struggling student in General Chemistry II who had a failing grade at the time. After working together for the rest of the semester, he passed the class.
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National Heart, Lung, and Blood Institute
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United States
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Research Services
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300 - 400 Employee
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Research Intern
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May 2018 - Aug 2018
Worked and communicated across several laboratories and disciplines to carry out my summer project: “Implementation and Testing of a Global Analysis Software (gDAI) for Lifetime Images with Multiple Components”. This project required the preparation of fluorescent proteins in bacteria and of fluorescent dyes for fluorescence lifetime imaging to benchmark gDAI. Performed regular fluorescence lifetime imaging and data analysis. Worked and communicated across several laboratories and disciplines to carry out my summer project: “Implementation and Testing of a Global Analysis Software (gDAI) for Lifetime Images with Multiple Components”. This project required the preparation of fluorescent proteins in bacteria and of fluorescent dyes for fluorescence lifetime imaging to benchmark gDAI. Performed regular fluorescence lifetime imaging and data analysis.
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Education
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Brown University School of Engineering
Doctor of Philosophy - PhD, Biomedical Engineering -
University of Maine
Bachelor's degree, Biomedical Engineering